Abstract
Huanglongbing (HLB) is considered one of the most important diseases in citrus industry, which currently has no cure. In Argentina, ‘Candidatus Liberibacter asiaticus’ (CLas) has been detected only in plant material. It is transmitted by Diaphorina citri, which is distributed in most of the country’s citrus regions. The aim of this study was to detect the presence of CLas in D. citri in Argentina. A total of 350 samples of D. citri were analyzed between 2012 and 2018 from different geographical locations from the northeast region of Argentina. A nested-PCR method using specific primers were used for the detection of CLas by amplification of a 1160-bp fragment from the 16S rRNA-encoding gene. Three positive samples to CLas of D. citri were detected from Ituzaingó, Corrientes. The positive PCR products were sequenced, and phylogenetic analysis were conducted. The samples were confirmed to belong to CLas. The sequences obtained in this study grouped with CLas sequences plant material from Argentina together with other sequences from the rest of the world. This is the first report of CLas in D. citri in Argentina.
References
Agostini JP, Preussler C, Outi Y et al (2015) Huanglongbing (HLB) ex greening. In: Rossini MN, Dummel DM, Agostini JP (eds) Plagas cuarentenarias de frutales de la República Argentina Avances en los resultados, 2015th edn. INTA, Buenos Aires, pp 183–198
Augier L, Gastaminza G, Lizondo M et al (2006) Presencia de Diaphorina citri (Hemiptera: Psyllidae) en el Noroeste Argentino (NOA). Rev Soc Entomol Argentina 65:67–68
Badaracco A, Redes FJ, Preussler CA, Agostini JP (2017) Citrus Huanglongbing in Argentina: detection and phylogenetic studies of ‘Candidatus Liberibacter asiaticus’. Australas Plant Pathol 46:1–5. https://doi.org/10.1007/s13313-017-0473-4
Bové JM (2006) Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. J Plant Pathol 88:7–37. https://doi.org/10.4454/jpp.v88i1.828
Buenahora J (2009) HLB: Aspectos generales de la enfermedad—Diaphorina citri: Avances de la investigación en Uruguay. Programa Citric, Uruguay
Cáceres S (2005) Presencia de Tamarixia radiata Waterson (Hymenoptera: Eulophidae), parasitoide del psílido asiático Diaphorina citri Kuwayama (Hemíptera: Sternorrhyncha: Psyllidae) en cultivos cítricos de Corrientes. In: VI Congreso Argentino de Entomología. Tucumán, Argentina, p 290
Cáceres S, Aguirre A, Miño V et al (2008) Líneas de acción para implementar el manejo de Diaphorina citri en Corrientes. In: Primera reunión de entomológos dedicados al estudio del HLB y su insecto vector. Corrientes Argentina, Bella Vista, pp 23–24
Chiyaka C, Singer BH, Halbert SE et al (2012) Modeling Huanglongbing transmission within a citrus tree. Proc Natl Acad Sci U S A 109:12213–12218. https://doi.org/10.1073/pnas.1208326109
da Graça JV, Korsten L (2004) Citrus Huanglongbing: review, present status and future strategies. Dis Fruits Veg Vol I I:229–245. https://doi.org/10.1007/1-4020-2606-4_4
da Graça JV, Douhan GW, Halbert SE et al (2016) Huanglongbing: an overview of a complex pathosystem ravaging the world’s citrus. J Integr Plant Biol 58:373–387. https://doi.org/10.1111/jipb.12437
Deng X, Zhou G, Li H, Chen J, Civerolo EL (2007) Nested-PCR Detection and Sequence Confirmation of ‘Candidatus Liberibacter asiaticus’ from Murraya paniculata in Guangdong, China. Plant Dis 91:1051
Fariña N (2017) HLB en Paraguay. SENAVE, Asuncion
Federcitrus (2018) Actividad Citrícola 2018
Gottwald TR (2010) Current epidemiological understanding of citrus Huanglongbing. Annu Rev Phytopathol. https://doi.org/10.1146/annurev-phyto-073009
Grafton-Cardwell EE, Stelinski LL, Stansly PA (2013) Biology and management of asian citrus Psyllid, vector of the Huanglongbing pathogens. Annu Rev Entomol 58:413–432. https://doi.org/10.1146/annurev-ento-120811-153542
Halbert SE, Núñez CA (2004) Distribution of the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Rhynchota: Psyllidae) in the Caribbean Basin. Florida Entomol 87:401–402. https://doi.org/10.1653/0015-4040(2004)087[0401:dotacp]2.0.co;2
Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinform Appl Note 17:754–755
Jagoueix S, Bové JM, Garnier M (1994) The phloem-limited bacterium of greening disease of citrus is a member of the alpha subdivision of the Proteobacteria. Int J Syst Bacteriol 44:379–386
Jagoueix S, Bové JM, Garnier M (1996) PCR detection of the two ‘Candidatus’ Liberibacter species associated with greening disease of citrus. Mol Cell Probes 10:43–50. https://doi.org/10.1006/mcpr.1996.0006
Jagoueix S, Bové J, Garnier M (1997) Comparison of the 16S/23S ribosomal intergenic regions of ‘Candidatus Liberibacter asiaticus’ and ‘Candidatus Liberibacter africanus’, the two species associated with citrus Huanglongbing (greening) disease. Int J Syst Bacteriol 47:227–227. https://doi.org/10.1099/00207713-47-1-224
Keremane ML, Ramadugu C, Castaneda A et al (2015) Report of ‘Candidatus Liberibacter caribbeanus’, a new citrus- and psyllid-associated Liberibacter from Colombia, South America
Kumar S, Stecher G, Li M et al (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Luis M, Collazo C, Llauger R et al (2009) Occurrence of citrus Huanglongbing in Cuba and association of the disease with ‘Candidatus liberibacter asiaticus’. J Plant Pathol 91:709–712
Manjunath KL, Halbert SE (2004) Asian Citrus Psyllids (Sternorrhyncha: Psyllidae) and greening disease of citrus: a literature review and assessment of risk in Florida. Florida Entomol 87:330–353
Munir S, He P, Wu Y et al (2018) Huanglongbing control: perhaps the end of the beginning. Microb Ecol 76:192–204. https://doi.org/10.1007/s00248-017-1123-7
Murray MG, Thompson WF (1988) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325. https://doi.org/10.1093/nar/16.5.2269
Rambaut A (2010) FigTree v1.3.1. Inst. Biol. Evol. Univ. Edimburgo, Edimburgo, Edinburgh
Ronquist F, Teslenko M, Van Der Mark P et al (2012) Mrbayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542. https://doi.org/10.1093/sysbio/sys029
Schapovaloff ME, Bustamante KM, Badaracco A, Agostini JP (2019) First record of Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae) in Misiones Province, Argentina. Braz J Biol 80:192–193. https://doi.org/10.1590/1519-6984.204669
Tapia S (2008) Detección y distribución de D. citri en Jujuy y Salta. In: Primera reunión de entomológos dedicados al estudio del HLB y su insecto vector. Corrientes Argentina, Bella Vista, pp 27–28
Teixeira DDC, Saillard C, Eveillard S et al (2005a) ‘Candidatus Liberibacter americanus’, associated with citrus Huanglongbing (greening disease) in São Paulo State, Brazil. Int J Syst Evol Microbiol 55:1857–1862. https://doi.org/10.1099/ijs.0.63677-0
Teixeira DDC, Danet JL, Eveillard S et al (2005b) Citrus Huanglongbing in São Paulo State, Brazil: PCR detection of the ‘Candidatus’ Liberibacter species associated with the disease. Mol Cell Probes 19:173–179. https://doi.org/10.1016/j.mcp.2004.11.002
Teixeira DC, Wulff NA, Martins EC et al (2008) A phytoplasma closely related to the pigeon pea witches’-broom phytoplasma (16Sr IX) is associated with citrus Huanglongbing symptoms in the state of São Paulo, Brazil. Phytopathology 98:977–984. https://doi.org/10.1094/PHYTO-98-9-0977
Vaccaro NC (1994) Comportamiento y difusión de la «Chicharrita de los Citrus» Diaphorina citri (Homoptera-Diaphoridae) en la zona citrícola de Entre Ríos, 3, vol 2
Vaccaro N, Bouvet JP (2008) Diaphorina citri y Tamarixia radiata. Actividades e Entre Ríos y sur de Corrientes. In: Primera reunión de entomológos dedicados al estudio del HLB y su insecto vector. Corrientes Argentina, Bella Vista, pp 29–30
Weisburg WG, Barns SM, Pelletie DA et al (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Wulff NA, Fassini CG, Marques VV et al (2019) Molecular characterization and detection of 16SrIII group phytoplasma associated with Huanglongbing symptoms. Phytopathology 109:366–374. https://doi.org/10.1094/PHYTO-03-18-0081-R
Acknowledgements
This work was supported by the Instituto Nacional de Tecnología Agropecuaria (INTA) and Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina (CONICET).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Badaracco, A., Redes, F.J., Bustamente, K.M. et al. First detection of positive ‘Candidatus Liberibacter asiaticus’ Diaphorina citri in Argentina. Australasian Plant Pathol. 51, 9–12 (2022). https://doi.org/10.1007/s13313-021-00817-w
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13313-021-00817-w