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Diversity within and among Ethiopian barley (Hordeum vulgare L.) landraces in resistance to barley net blotch (Pyrenophora teres F. teres (Ptt))

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Abstract

In this study, we evaluated a collection of 585 barley landraces along with 10 checks at Sinana Agricultural Research Center (SARC) and Bale-Goba research sites in 2018/19 and 2019/20 cropping seasons for resistance to net blotch (Pyrenophora teres F. teres (Ptt)). The experiments were laid out in an augmented complete block design. Data on agronomic performance and disease response were recorded. Analysis of variance showed highly significant variations between and within the landraces and cultivars in net blotch percent severity index (PSI), area under the disease progress curve (AUDPC), apparent infection rate, days to heading, plant height, number of fertile tillers per plant, seeds per spike, spike length and thousand seed weight. The overall mean net blotch epidemics varied from area under disease progress curve (AUDPC) of 86 to 1835. The disease was as high as AUDPC of 1378 on the susceptible check ‘Abdanie’. Similarly, the variations between and within barley landraces in terms of regions of origin, altitude classes and ear-types were significant. Out of the 585 barley landraces evaluated, 198 (33.85%) had significantly lower disease than the susceptible check, of which 12 (2.05%) and 186 (31.79%) landraces, respectively showed resistant (R) and moderately resistant (MR) infection types. The frequency of resistant landraces was more in third (2000-2500 m) and fourth (>2500 m) altitude classes and six-rowed and irregular barley types than in lower altitude areas and two-rowed ear-types. Furthermore, the correlation analysis among PSI and some yield components revealed adverse effect of the disease on grain yield of barley.

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Acknowledgments

The authors would like to thank Ethiopian Biotechnology Institute for funding this research, Ethiopian Biodiversity Institute (EBI) for provision of landraces, Sinana and Holetta Agricultural Research Center for provision of research site and improved checks. Mr. Debale Tsegaye is acknowledged for his technical support in field data collection.

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Authors and Affiliations

  1. Ethiopian Biotechnology Institute, P. O. Box 32853, Addis Ababa, Ethiopia

    Allo A. Dido & Kassahun Tesfaye

  2. Department of Bio-Technology, KL University, Green Field, Vaddeswaram, PIN 522-502, Guntur, Andhra Pradesh, India

    Allo A. Dido, M. S. R. Krishna & B. J. K. Singh

  3. Ethiopian Institute of Agricultural Research, Plant Biotechnology Research Program, Melkasa Agricultural Research Centre, P. O. Box 436, Adama, Ethiopia

    Dawit T. Degefu

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  1. Allo A. Dido
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  2. Kassahun Tesfaye
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  3. M. S. R. Krishna
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  4. Dawit T. Degefu
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  5. B. J. K. Singh
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Contributions

AAD, KT and DTD planned and supervised the experimental work; AAD, DTD and KT performed traits evaluation over the two years (Field experiments) and over the two experimental sites; AAD, KT and DTD analyzed the data; AAD, DTD, MSRK and BJKS drafted the manuscript; all authors read and approved the final version of the manuscript.

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Correspondence to Allo A. Dido.

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Dido, A.A., Tesfaye, K., Krishna, M.S.R. et al. Diversity within and among Ethiopian barley (Hordeum vulgare L.) landraces in resistance to barley net blotch (Pyrenophora teres F. teres (Ptt)). Australasian Plant Pathol. 50, 217–227 (2021). https://doi.org/10.1007/s13313-020-00764-y

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