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Development of a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of sweet potato latent virus-lotus in lotus plants

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Abstract

A previous survey of lotus virus disease has revealed sweet potato latent virus-lotus (SPLV-lotus) to occur at high frequencies in Jiangsu Province, China. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of SPLV-lotus in lotus plants. The products were analysed by visual inspection of colour change. The sensitivity of the LAMP assay was similar to that of RT-PCR. The LAMP assay shows excellent potential for application in the rapid detection of SPLV-lotus in field samples.

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Acknowledgements

This work was supported by grants from the Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302), China Agriculture Research System (CARS-24) and National Natural Science Foundation of China (No. 31601604).

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Authors and Affiliations

  1. School of Horticulture and Plant Protection, Yangzhou University, Wenhui East Road No.48, Jiangsu Province, 225009, Yangzhou, People’s Republic of China

    Zhen He, Tingting Dong, Weiwen Wu & LiangJun Li

  2. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Wenhui East Road No.48, Jiangsu Province, 225009, Yangzhou, People’s Republic of China

    Zhen He & LiangJun Li

  3. National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, 100700, Beijing, People’s Republic of China

    Tielin Wang

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  1. Zhen He
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  2. Tingting Dong
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  3. Weiwen Wu
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  4. Tielin Wang
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  5. LiangJun Li
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Correspondence to LiangJun Li.

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He, Z., Dong, T., Wu, W. et al. Development of a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of sweet potato latent virus-lotus in lotus plants. Australasian Plant Pathol. 49, 425–429 (2020). https://doi.org/10.1007/s13313-020-00714-8

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  • DOI: https://doi.org/10.1007/s13313-020-00714-8

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