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A novel multiplex PCR tool for simultaneous detection of three root-knot nematodes

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Abstract

Root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria, cause serious yield losses in protected growing areas in Turkey. They also show a‘co-infection’ profile in fields’ soil microflora. In this study, we designed multiplex primers for detecting all three species simultaneously. Additionally, the confidence and definition of this method were tested by using mixed and large populations related to these species at different biological stages. We suggest this effective tool be used to easily identify three root-knot nematodes that will be beneficial for designing crop rotation modelling in protected growing areas in Turkey as well as quarantine requirements.

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Acknowledgments

The research was financially supported by The Scientific and Technological Research Council of Turkey project no 112O116.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, 07058, Antalya, Turkey

    Zübeyir Devran & İbrahim Mıstanoğlu

  2. Batı Akdeniz Agricultural Research Institute, 07100, Antalya, Turkey

    İlknur Polat

  3. Faculty of Science, Department of Molecular Biology and Genetics, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    Ömür Baysal

Authors
  1. Zübeyir Devran
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  2. İlknur Polat
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  3. İbrahim Mıstanoğlu
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  4. Ömür Baysal
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Correspondence to Zübeyir Devran.

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Devran, Z., Polat, İ., Mıstanoğlu, İ. et al. A novel multiplex PCR tool for simultaneous detection of three root-knot nematodes. Australasian Plant Pathol. 47, 389–392 (2018). https://doi.org/10.1007/s13313-018-0570-z

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  • DOI: https://doi.org/10.1007/s13313-018-0570-z

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