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Screening lentil germplasm for stemphylium blight resistance

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Abstract

Stemphylium blight is an important fungal disease of lentil caused by Stemphylium botryosum. It occurs in all major lentil growing regions of the world, including Bangladesh, India, Nepal, Syria, USA and Canada. As lentil production in Australia is increasing, stemphylium blight is considered a potential threat to the industry. However, information on this pathogen and resistance levels in Australian lentil germplasm is limited. Five fungicides were tested for efficacy against seven isolates of Stemphylium botryosum. Mancozeb and thiram were the most effective in inhibiting the growth of the pathogen under in-vitro conditions. To prevent a disease outbreak, it is prudent to screen germplasm to eliminate stemphylium blight susceptible lines from the breeding program prior to release. Therefore, a screening method for evaluating stemphylium blight resistance in lentil was developed for use under controlled environment conditions. Using this method, stemphylium blight disease ratings were provided on all current Australian lentil varieties. In addition, screening of advanced breeding lines showed variation in resistance/susceptibility for this disease and allowed selection of resistant lines for stemphylium blight resistance breeding. Seven International lentil varieties were tested against Australian isolates of S. botryosum and showed varying levels of resistance. Three hundred lentil accessions obtained from the International Centre for Agricultural Research in the Dry Areas (ICARDA) were evaluated and six accessions were identified with higher resistance than the resistant check ILL6002 that could be used in lentil breeding for stemphylium blight resistance.

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Acknowledgements

This work is a part of Project No DAV00117 ‘Pulse Germplasm Enhancement, Resistance to biotic stresses’ funded by the Grains Research and Development Corporation and DEDJTR, Victoria. We thank Dr. Trevor Bretag, Dr. Grant Hollaway and Dr. Jacky Edwards for reviewing the manuscript and Mr. Alistair Smith for his assistance. The Fungicide efficacy protocol was provided by Ms. Kasia Rybak, Centre for Crop and Disease Management, Department of Environment and Agriculture, Curtin University, Bentley WA 6102, Australia.

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Authors and Affiliations

  1. Department of Economic Development, Jobs, Transport & Resources, Grains Innovation Park, 110 Natimuk Road, Horsham, VIC, 3401, Australia

    Pragya Kant, Michael Materne & Matthew S. Rodda

  2. Department of Economic Development, Jobs, Transport & Resources, AgriBio, 5 Ring Road, Bundoora, VIC, 3083, Australia

    Anthony T. Slater

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  1. Pragya Kant
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  2. Michael Materne
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  3. Matthew S. Rodda
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  4. Anthony T. Slater
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Correspondence to Pragya Kant.

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Kant, P., Materne, M., Rodda, M.S. et al. Screening lentil germplasm for stemphylium blight resistance. Australasian Plant Pathol. 46, 129–136 (2017). https://doi.org/10.1007/s13313-017-0469-0

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  • DOI: https://doi.org/10.1007/s13313-017-0469-0

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