We report the first fully sequenced genome of an Australian tomato spotted wilt virus isolate (TSWV-QLD1). The tripartite genome consists of an 8914 nt L segment, a 4869 nt M segment, and a 3003 nt S segment. Phylogenetic analysis of the N protein sequence places this Australian isolate in a different clade than partially sequenced TSWV isolates from other regions of Australia.
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de Avila AC, de Haan P, Kormelink R, Resende Rde O, Goldbach RW, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. J G Virol 74(Pt 2):153–159
de Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of tomato spotted wilt virus has an ambisense character. J G Virol 71(Pt 5):1001–1007
de Haan P, Kormelink R, de Oliveira RR, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J G Virol 72(Pt 9):2207–2216
Debreczeni DE, Lopez C, Aramburu J, Daros JA, Soler S, Galipienso L, Falk BW, Rubio L (2015) Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Arch Virol 160(8):2117–2123
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M et al (2008) Phylogeny.Fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36(Web Server issue):W465–469
Hu ZZ, Feng ZK, Zhang ZJ, Liu YB, Tao XR (2011) Complete genome sequence of a tomato spotted wilt virus isolate from China and comparison to other TSWV isolates of different geographic origin. Arch Virol 156(10):1905–1908
Kikkert M, Verschoor A, Kormelink R, Rottier P, Goldbach R (2001) Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells. J Virol 75(2):1004–1012
Kormelink R, de Haan P, Meurs C, Peters D, Goldbach R (1992) The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J G Virol 73(Pt 11):2795–2804
Kormelink R, Storms M, Van Lent J, Peters D, Goldbach R (1994) Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200(1):56–65
Lee JS, Cho WK, Kim MK, Kwak HR, Choi HS, Kim KH (2011) Complete genome sequences of three tomato spotted wilt virus isolates from tomato and pepper plants in Korea and their phylogenetic relationship to other TSWV isolates. Arch Virol 156(4):725–728
Lewandowski DJ, Adkins S (2005) The tubule-forming NSm protein from tomato spotted wilt virus complements cell-to-cell and long-distance movement of tobacco mosaic virus hybrids. Virology 342(1):26–37
Lian S, Lee JS, Cho WK, Yu J, Kim MK, Choi HS, Kim KH (2013) Phylogenetic and recombination analysis of tomato spotted wilt virus. PLoS One 8(5):e63380
Lopez C, Aramburu J, Galipienso L, Soler S, Nuez F, Rubio L (2011) Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of Tomato spotted wilt virus. J G Virol 92(Pt 1):210–215
Margaria P, Rosa C (2015) First complete genome sequence of a tomato spotted wilt virus isolate from the United States and its relationship to other TSWV isolates of different geographic origin. Arch Virol 160(11):2915–2920
Margaria P, Ciuffo M, Pacifico D, Turina M (2007) Evidence that the nonstructural protein of tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the TSW gene. Mol Plant-Microbe Interact: MPMI 20(5):547–558
Margaria P, Miozzi L, Ciuffo M, Pappu HR, Turina M (2015) The first complete genome sequences of two distinct European tomato spotted wilt virus isolates. Arch Virol 160(2):591–595
Mitter N, Koundal V, Williams S, Pappu H (2013) Differential expression of tomato spotted wilt virus-derived viral small RNAs in infected commercial and experimental host plants. PLoS One 8(10):e76276
Moyle RL, Birch RG (2013) Diversity of sequences and expression patterns among alleles of a sugarcane loading stem gene. Theor Appl Genet 126(7):1775–1782
Pappu H, Pappu S, Jain R, Bertrand P, Culbreath A, McPherson R, Csinos A (1998) Sequence characteristics of natural populations of tomato spotted wilt tospovirus infecting flue-cured tobacco in Georgia. Virus Genes 17(2):169–177
Pappu HR, Jones RA, Jain RK (2009) Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Res 141(2):219–236
Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okuno T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532(1–2):75–79
Ullman DE, German TL, Sherwood JL, Westcot DM, Cantone FA (1993) Tospovirus replication in insect vector cells - Immunocytochemical evidence that the nonstructural protein encoded by the S-Rna of tomato spotted wilt tospovirus is present in thrips vector cells. Phytopathology 83(4):456–463
Wijkamp I, Vanlent J, Kormelink R, Goldbach R, Peters D (1993) Multiplication of tomato spotted wilt virus in its insect vector, Frankliniella-Occidentalis. J Gen Virol 74:341–349
This study was funded by the Australian Research Council (Discovery project grant number DP140103363).
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The authors declare no conflict of interest.
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Moyle, R., Pretorius, L., Dalton-Morgan, J. et al. Analysis of the first complete genome sequence of an Australian tomato spotted wilt virus isolate. Australasian Plant Pathol. 45, 509–512 (2016). https://doi.org/10.1007/s13313-016-0435-2
- small RNA sequencing
- Sanger sequencing