Abstract
Oomycete pathogens, such as Phytophthora sojae, encode several hundreds of RXLR effectors to manipulate host immunity responses. For these effectors transporting into the host cells, P. sojae may produce PtdIns(3)P to bind the effectors. Transgenic P. sojae that expressed PtdIns(3)P-specific fluorescent probes were used for PtdIns(3)P distribution in parasite. Fluorescence was associated mainly with the endoplasmic reticulum (ER), where effectors might to bind PtdIns(3)P for transport. Furthermore, PtdIns(3)P biosensor was detected that enriched in haustoria during infection. We also identified three PsPI3Ks functionally complement yeast VPS34, which generates only PtdIns(3)P. These results further demonstrated that P. sojae might generated PtdIns(3)P to benefit their infectious process.
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This project is supported by grants from the Special Fund for Agro-scientific Research in the Public Interest (No.201503112).
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Table S1
Oligonucleotides used in the study. (DOC 40 kb)
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Chen, L., Wang, W., Hou, Y. et al. Phosphatidylinositol 3-phosphate, an essential lipid in Phytophthora sojae, enriches in the haustoria during infection. Australasian Plant Pathol. 45, 435–441 (2016). https://doi.org/10.1007/s13313-016-0430-7
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DOI: https://doi.org/10.1007/s13313-016-0430-7