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Population structure of Holocryphia capensis (cryphonectriaceae) from Metrosideros angustifolia and its pathogenicity to Eucalyptus species

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Abstract

Holocryphia capensis is a recently described fungus that infects native Metrosideros angustifolia (Myrtaceae, Myrtales) trees in South Africa. This species is related to a number of serious canker pathogens of trees in the Cryphonectriaceae. An ability to produce lesions on M. angustifolia in artificial inoculation studies and its close relationship to the introduced eucalypt pathogen, H. eucalypti, raises concerns as to its diversity and possible impact on Myrtaceae in Southern Africa. Eight microsatellite markers were used to study the genetic diversity of H. capensis isolates from four sites in the Western Cape Province. All isolates could be assigned to one population, with very low gene and genotypic diversity. This was despite the fact that sexual structures of the fungus are found on infected trees. The results suggest that H. capensis could have been introduced into the Western Cape from a single, as yet unknown origin. Pathogenicity tests showed that H. capensis is pathogenic to the tested Eucalyptus clone.

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Acknowledgments

We thank the DST/NRF Centre of Excellence in Tree Health Biotechnology (CHTB) for financial support. We are grateful to Dr. Francois Roets and Ms. Tendai Musvuugwa from Stellenbosch University, as well as Mrs. Jane Forrester from Harold Porter National Botanical Gardens for assistance with field sampling, and colleagues Dr. DongHyeon Lee and Dr. MingLiang Yin in FABI for their valuable assistance on inoculation trials. We also acknowledge the Western Cape Nature Conservation Board for issuing the necessary collecting permits.

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Authors and Affiliations

  1. DST/NRF Centre of Excellence in Tree Health Biotechnology (CTHB), Forestry and Agricultural Biotechnology Institute (FABI), Department of Microbiology and Plant Pathology, University of Pretoria, Private Bag X20, Pretoria, 0028, South Africa

    ShuaiFei Chen, Michael J. Wingfield & Jolanda Roux

  2. DST/NRF Centre of Excellence in Tree Health Biotechnology (CTHB), Forestry and Agricultural Biotechnology Institute (FABI), Department of Genetics, University of Pretoria, Private Bag X20, Pretoria, 0028, South Africa

    Nicolaas A. van der Merwe

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  1. ShuaiFei Chen
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  2. Nicolaas A. van der Merwe
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  4. Jolanda Roux
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Correspondence to Jolanda Roux.

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Chen, S., van der Merwe, N.A., Wingfield, M.J. et al. Population structure of Holocryphia capensis (cryphonectriaceae) from Metrosideros angustifolia and its pathogenicity to Eucalyptus species. Australasian Plant Pathol. 45, 201–207 (2016). https://doi.org/10.1007/s13313-016-0399-2

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  • DOI: https://doi.org/10.1007/s13313-016-0399-2

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