Abstract
Curvularia lunata produces 1,8-dihydroxynaphthalene (DHN) melanin. Previously, we have successfully cloned and silenced the Brn1 gene, which is involved in DNH melanin production and pathogenicity. However, we hypothesise that other proteins may also be related to melanin production or pathogenicity. In this study, a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry techniques was used to identify the specific proteins associated with Brn1 expression in C. lunata wild-type CX-3 and Brn1-silenced mutant T5. One up-regulated and seven down-regulated proteins were successfully identified in T5. Whilst transaldolase, NADP-dependent mannitol dehydrogenase, formate dehydrogenase, and UTP-glucose-1-phosphate uridylyltransferase are involved in energy metabolism, elongation factor 3 facilitates the synthesis of important proteins required for the production of melanin and stress tolerance-related proteins (HSP30 and HSP70) and catalase (CAT) contribute to the pathogenicity of C. lunata. Of these proteins, CAT was further analysed as a protein of interest. Bioinformatics analysis showed that CAT consists of 748 amino acids and clustered into one branch with Dothideomycete CAT homologues in a phylogenetic tree. Further research on the exact role of the CAT gene is underway.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31171798, 31471734) and the China Agriculture Research System (CARS-02).
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Jin-Xin Gao and Jing Jing contributed equally to this work.
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Gao, JX., Jing, J., Liu, T. et al. Identification of proteins associated with the production of melanin and with pathogenicity in maize pathogen Curvularia lunata . Australasian Plant Pathol. 44, 599–603 (2015). https://doi.org/10.1007/s13313-015-0382-3
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DOI: https://doi.org/10.1007/s13313-015-0382-3