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A PCR detection system for South African basidiomycetous isolates from esca affected grapevine

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Abstract

Grapevine trunk diseases threaten the economic livelihood and lifespan of vineyards. One such disease, esca, involves the fungal colonisation and consequent blocking of vascular tissue, followed by tissue necrosis and eventual wood rot. Basidiomycetous fungi are the main causal agents of this wood rot and phylogenetic analysis group all South African isolates into ten taxa all belonging to the Hymenochaetales. Genera represented include Inocutis, Inonotus, Fomitiporella, Fomitiporia and Phellinus. To be able to identify large numbers of unidentified basidiomycetous field isolates, species-specific primers were developed to identify the ten taxa with a PCR assay based on the ribosomal ITS1 and ITS2 region. Furthermore isolates from fruiting bodies found on non-Vitis sp. woody hosts were identified as Fomitiporia capensis by using this newly developed assay.

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Acknowledgments

Wine Industry Network for Expertise and Technology (Winetech), and the National Research Foundation (NRF) are thanked for funding. Professional Development Programme (PDP) Postdoctoral fellowship (NRF awarded) to M C Bester. Dr Michael Fischer for providing strains and Dr Mia Cloete for valuable discussions. Martin Smit for permission and assistance with the sampling of ornamental trees in the Stellenbosch University botanical garden.

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Authors and Affiliations

  1. Department of Plant Pathology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

    Michael C. Bester, Francois Halleen & Lizel Mostert

  2. Plant Protection Division, ARC Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa

    Michael C. Bester & Francois Halleen

Authors
  1. Michael C. Bester
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  2. Francois Halleen
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  3. Lizel Mostert
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Corresponding author

Correspondence to Lizel Mostert.

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Suppl. Fig. 1

Specimens found on non-grapevine hosts from which the three Fomitiporia capensis isolates (STEU 7806, STEU 7807 and STEU 7808) were isolated from (a) STEU 7806; host: Podocarpus sp. (from Jonkershoek state forest, Stellenbosch), (b) STEU 7807; host: Prunus cerasifera (from Botanical gardens, Stellenbosch), (c) STEU 7808; host: Ehretia rigida (from Botanical gardens, Stellenbosch). (PNG 1345 kb)

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Bester, M.C., Halleen, F. & Mostert, L. A PCR detection system for South African basidiomycetous isolates from esca affected grapevine. Australasian Plant Pathol. 44, 647–651 (2015). https://doi.org/10.1007/s13313-015-0378-z

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  • DOI: https://doi.org/10.1007/s13313-015-0378-z

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