Abstract
The wheat variety Mace is currently dominating the southern wheat growing regions of Australia. It is high yielding in most environments and resistant to many diseases including yellow spot (also known as tan spot). However, observations of foliar yellowing of Mace have recently been reported in the field. This has raised concerns over a possible breakdown of resistance to yellow spot, which is caused by the necrotrophic fungal pathogen Pyrenophora tritici-repentis. West Australian field samples of yellowing Mace leaves were evaluated for P. tritici-repentis infection, and this pathogen was determined to be absent. Instead, Alternaria spp. were isolated from the wheat leaves. Pathogenicity assays showed that the recovered Alternaria spp. were unable to cause disease symptoms on Mace. Furthermore, spontaneous foliar lesions were observed in Mace grown in the absence of pathogens. It is therefore likely that such yellowing is a physiological trait, which will not respond to fungicide application. A marginal impact on yield cannot be excluded.
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References
AGT (2013a) Mace: Wheat variety fact sheet for SA and western Victoria. http://www.ausgraintechcom/uploads/varietyfactsheets2013/MaceSAwVicAugust2013web.pdf. Accessed March 2014
AGT (2013b) Mace: Wheat variety fact sheet for WA. http://www.ausgraintech.com/uploads/varietyfactsheets2013/MaceWAAugust2013web.pdf. Accessed March 2014
AGT (2013c) Yellowing in wheat. http://www.ausgraintech.com/uploads/agro fact sheets/Yellowing in wheat August 2013 web.pdf. Accessed March 2014
Antoni EA, Rybak K, Tucker MP, Hane JK, Solomon PS, Drenth A, Shankar M, Oliver RP (2010) Ubiquity of ToxA and absence of ToxB in Australian populations of Pyrenophora tritici-repentis. Australas Plant Pathol 39(1):63–68. doi:10.1071/Ap09056
Birchip Cropping Group (2013) Yellowing in Kord wheat. http://www.bcg.org.au/cb_pages/news/Hotagronomy.php. Accessed March 2014
Brugger EM, Wagner J, Schumacher DM, Koch K, Podlech J, Metzler M, Lehmann L (2006) Mutagenicity of the mycotoxin alternariol in cultured mammalian cells. Toxicol Lett 164(3):221–230. doi:10.1016/j.toxlet.2006.01.001
DAFWA (2013a) PestFax Issue 15. http://archive.agric.wa.gov.au/objtwr/imported_assets/content/pw/pestfax_no.15_(16_aug_13).pdf. Accessed March 2014
DAFWA (2013b) PestFax Issue 18. http://archive.agric.wa.gov.au/objtwr/imported_assets/content/pw/pestfax_no.18_(06_sept_2013).pdf. Accessed March 2014
DAFWA (2013c) What are the risks of growing too much Mace? http://archive.agric.wa.gov.au/PC_95462.html?s=1001. Accessed May 2014
DAFWA (2013d) Wheat variety guide for WA 2013. http://archive.agric.wa.gov.au/objtwr/imported_assets/content/amt/agb/wheat_variety_guide_2013.pdf. Accessed March 2014
DAFWA (2014) Wheat Variety Guide for WA 2014 http://grdc.com.au/Resources/Publications/2014/02/Wheat-Variety-Guide-for-WA-2014. http://grdc.com.au/Resources/Publications/2014/02/Wheat-Variety-Guide-for-WA-2014. Accessed April 2014
Fehr M, Pahlke G, Fritz J, Christensen MO, Boege F, Altemoller M, Podlech J, Marko D (2009) Alternariol acts as a topoisomerase poison, preferentially affecting the II alpha isoform. Mol Nutr Food Res 53(4):441–451. doi:10.1002/mnfr.200700379
Lehmann L, Wagner J, Metzler M (2006) Estrogenic and clastogenic potential of the mycotoxin alternariol in cultured mammalian cells. Food Chem Toxicol 44(3):398–408. doi:10.1016/j.fct.2005.08.013
Mitakakis TZ, Clift A, McGee PA (2001) The effect of local cropping activities and weather on the airborne concentration of allergenic Alternaria spores in rural Australia. Grana 40(4–5):230–239. doi:10.1080/001731301317223268
Moffat CS, See PT, Oliver RP (2014) Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis. Mol Plant Pathol 15(9):918–926. doi:10.1111/mpp.12154
Murray GM, Brennan JP (2009) Estimating disease losses to the Australian wheat industry. Australas Plant Pathol 38(6):558–570. doi:10.1071/Ap09053
Oviedo MS, Sturm ME, Reynoso MM, Chulze SN, Ramirez ML (2013) Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat. Braz J Microbiol Publ Braz Soc Microbiol 44(2):447–455. doi:10.1590/S1517-83822013000200017
Perello A, Moreno M, Sisterna M (2008) Alternaria infectoria species-group associated with black point of wheat in Argentina. Plant Pathol 57(2):379. doi:10.1111/j.1365-3059.2007.01713.x
Pfeiffer E, Eschbach S, Metzler M (2007) Alternaria toxins: DNA strand-breaking activity in mammalian cellsin vitro. Mycotoxin Res 23(3):152–157. doi:10.1007/BF02951512
SARDI (2014) Sowing Guide 2014: What to sow next season. http://www.grdc.com.au/~/media/D9A0EB71B6264BFFA9793D16CF456762.pdf. Accessed March 2014
Schreck I, Deigendesch U, Burkhardt B, Marko D, Weiss C (2012) The Alternaria mycotoxins alternariol and alternariol methyl ether induce cytochrome P450 1A1 and apoptosis in murine hepatoma cells dependent on the aryl hydrocarbon receptor. Arch Toxicol 86(4):625–632. doi:10.1007/s00204-011-0781-3
Shipton WA, Chambers SC (1966) The internal microflora of wheat grains in Western Australia. Aust J Exp Agric Anim Husb 6:432–436
Singh D, Sharma A, Tewari AN, Singh KP, Singh AK, Singh RN, Singh SP, Kalappanawar IK, Dodan DS, Singh VK (2004) Assessment of losses due to leaf blight in popular varieties of wheat (Triticum aestivum) under different sowing conditions and agroclimatic zones in India. Indian J Agric Sci 74(2):110–113
Singh DP, Sharma AK, Kumar P, Chowdhury AK, Singh KP, Mann SK, Singh RN, Singh AK, Kalappanavar IK, Tewari AN (2008) Management of leaf blight complex of wheat (Triticum aestivum) caused by Bipolaris sorokiniana and Alternaria triticina in different agroclimatic zones us an integrated approach. Indian J Agric Sci 78(6):513–517
Thomma BP (2003) Alternaria spp.: from general saprophyte to specific parasite. Mol Plant Pathol 4(4):225–236. doi:10.1046/j.1364-3703.2003.00173.x
Vergnes DM, Renard ME, Duveiller E, Maraite H (2006) Identification of Alternaria spp. on wheat by pathogenicity assays and sequencing. Plant Pathol 55(4):485–493. doi:10.1111/j.1365-3059.2006.01391.x
Wallwork H (2011) Cereal disease update SA 2011. GRDC Update Papers. https://www.grdc.com.au/Research-and-Development/GRDC-Update-Papers/2011/02/Cereal-disease-update-SA-2011. Accessed March 2014
Webley DJ, Jackson KL, Mullins JD, Pitt JI, Hocking AD, Miscamble B, Ward C, Bull M, Mirkazemi J (1995) Mycotoxins and toxigenic fungi in Australian grain. Grains Research and Development Corporation Report
Webley DJ, Jackson KL, Mullins JD, Hocking AD, Pitt JI (1997) Alternaria toxins in weather-damaged wheat and sorghum in the 1995–1996 Australian harvest. Aust J Agric Res 48(8):1249–1255. doi:10.1071/A97005
Wheeler R (2012) New wheat varieties dominate 2012 trials. SARDI Crop Performance Report 2012 http://www.sardi.sa.gov.au/__data/assets/pdf_file/0005/185054/VIT_016_SARDI_Guide_April_2013_WHEAT_WEB.pdf. Accessed March 2014
White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Protocols PCR (ed) A guide to methods and applications. Academic Press, Inc., New York, pp 315–322
Young K (2013) Wheat 2012 NVT trial results and key agronomy issues. GRDC Update Papers. http://www.grdc.com.au/Research-and-Development/GRDC-Update-Papers/2013/03/Wheat-2012-NVT-trial-results-and-key-agronomy-issues. Accessed March 2014
Acknowledgments
This work was supported by grants from the Australian Grains Research and Development Corporation (GRDC). We thank J. Reinheimer (Australian Grain Technologies) and M. Shankar (Department of Agriculture and Food, WA) for provision of Mace field samples. We also thank the Australian Winter Cereals Collection (AWCC) for the provision of seeds.
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Moffat, C.S., See, P.T. & Oliver, R.P. Leaf yellowing of the wheat cultivar Mace in the absence of yellow spot disease. Australasian Plant Pathol. 44, 161–166 (2015). https://doi.org/10.1007/s13313-014-0335-2
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DOI: https://doi.org/10.1007/s13313-014-0335-2