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The population structure of Hemileia vastatrix in Brazil inferred from AFLP

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Abstract

Efforts to obtain durable resistant cultivars to coffee leaf rust have a long history of disappointment because of the lack of information on the Hemileia vastatrix evolutionary potential. In this study, the AFLP marker was used to analyze the genetic structure of a field population of H. vastatrix from different coffee genotypes cultivated in a wide geographic region of Brazil’s main production areas. A total of 91 isolates were sampled from genotypes of Coffea arabica, C. canephora and Híbrido de Timor/Icatu derivatives. Each isolate exhibited unique multilocus AFLP genotypes with no identical isolates obtained from the same geographical and host origins. This finding was corroborated by a small correlation between genetic similarity and geographic distance (R xy  = 0.31, P = 0.23). The analyses showed a low genetic differentiation (G ST  = 0.026) between the populations defined by a host and AMOVA analysis, most of the genetic variance (99.56 %) was attributed to differences within populations. In addition, based on the index of association (IA), the random mating possibility was not rejected (IA = 0.225, P = 0.123) for the population of H. vastatrix derived from C. canephora. This result suggested that H. vastatrix in Brazil behaves as a large undifferentiated population with a high level of genotypic diversity that is unstructured with regard to its geographic and host origins.

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Acknowledgments

The authors thank the Programa Nacional de Pesquisa e Desenvolvimento de Café (PNP&D/CAFÉ/EMBRAPA CAFÉ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) for financial support.

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Authors and Affiliations

  1. Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil

    T. A. Maia, E. Maciel-Zambolim, E. S. G. Mizubuti & L. Zambolim

  2. Embrapa Café, Brasília, Distrito Federal, 70770-901, Brazil

    E. T. Caixeta

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  1. T. A. Maia
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  2. E. Maciel-Zambolim
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  3. E. T. Caixeta
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  4. E. S. G. Mizubuti
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  5. L. Zambolim
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Correspondence to L. Zambolim.

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Maia, T.A., Maciel-Zambolim, E., Caixeta, E.T. et al. The population structure of Hemileia vastatrix in Brazil inferred from AFLP. Australasian Plant Pathol. 42, 533–542 (2013). https://doi.org/10.1007/s13313-013-0213-3

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