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Phyllosticta spp. on cultivated Citrus in Australia

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Abstract

The occurrence of pathogenic and endophytic species of Phyllosticta on cultivated Citrus in Australia was investigated by DNA sequence analysis of specimens held in plant pathology herbaria and culture collections. Sequences of the internal transcribed spacer region (ITS1, 5.8S, ITS2), and partial translation elongation factor 1-alpha (TEF) gene of 41 Phyllosticta-like isolates from Citrus were compared to those sequences from the type specimens of Phyllosticta recorded from around the world. Phylogenetic analysis resolved all the sequences of Australian accessions into two major clades. One clade corresponded to P. citricarpa, which causes citrus black spot disease. The other clade contained P. capitalensis, which is a known endophyte of Citrus and many other plant species. All included herbarium accessions previously designated as Guignardia mangiferae are now designated P. capitalensis. No Australian isolates were identified as the newly described pathogens of citrus P. citriasiana or P. citrichinaensis, or the endophytes Guignarida mangiferae, P. brazilianiae, or P. citribraziliensis.

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Acknowledgments

The authors gratefully acknowledge the financial support from Bioresources Queensland, Citrus Australia Limited, and Horticulture Australia Limited. Thank you to Dr Michael Priest and Karren Cowan for the supply of cultures from the NSW DPI Plant Pathology Herbarium. Thank you to Dr Roger Shivas from the Queensland Plant Pathology Herbarium. Mark Hoult and Mark Traynor of the Northern Territory Department of Resources.

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Authors and Affiliations

  1. Department of Agriculture, Fisheries and Forestry (Queensland), Ecosciences Precinct, 41 Boggo Road, Dutton Park, Qld, 4102, Australia

    A. K. Miles & Y. P. Tan

  2. NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Private Bag 4008, Narellan, NSW, 2567, Australia

    M. K. Tan, N. J. Donovan & A. Ghalayini

  3. The University of Queensland, Centre for Plant Science, Ecosciences Precinct, 41 Boggo Road, Dutton Park, Qld, 4102, Australia

    A. Drenth

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  1. A. K. Miles
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  2. Y. P. Tan
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  3. M. K. Tan
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  4. N. J. Donovan
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  5. A. Ghalayini
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  6. A. Drenth
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Correspondence to A. K. Miles.

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Miles, A.K., Tan, Y.P., Tan, M.K. et al. Phyllosticta spp. on cultivated Citrus in Australia. Australasian Plant Pathol. 42, 461–467 (2013). https://doi.org/10.1007/s13313-013-0208-0

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  • DOI: https://doi.org/10.1007/s13313-013-0208-0

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