The potato cyst nematodes (PCN), Globodera rostochiensis (Woll.) and G. pallida (Stone), are major pests of ware and seed potato (Solanum tuberosum L.) crops worldwide and severely impact the movement of potatoes around the globe through quarantine restrictions. In Australia, only G. rostochiensis has been discovered, on four separate occasions between 1986 and 2008. The infested areas are the subject of strict regulation and quarantine procedures and while they are considered to be contained, managing nematode populations remains a priority. This study has identified the G. rostochiensis Ro1 resistance-status of potato cultivars currently grown by Australian potato growers, and new cultivars emerging from the Australian Potato Breeding Program. Resistance was assessed by a simple and robust procedure carried out in a purpose-built quarantine facility. Of the 24 potato cultivars grown in the affected Koo Wee Rup district in 2004, 10 were resistant to nematode infestation, including the locally important cultivar Atlantic. Other cultivars important to the Victorian and Australian potato industry, such as Kennebec, Desiree, Sebago and Coliban, were classified as susceptible. Importantly, this study provided evidence that the Koo Wee Rup PCN population was able to complete its lifecycle on the native plant species, S. aviculare (kangaroo apple), potentially acting as an alternate host and spreading PCN among potato crops.
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Berg G, Merriman P, Sully R (2004) Australian potato cyst management plan; Revised draft, Horticulture Australia Ltd
Brodie BB (1996) Effect of initial nematode density on managing Globodera rostochiensis with resistant cultivars and non-hosts. J Nematol 28:510–519
Brodie BB, Brucato ML (1993) Effects of inoculum density and egg age on establishment of Globodera rostochiensis populations. J Nematol 25:286–290
Brown EB, Sykes GB (1983) Assessment of the losses caused to potatoes by the potato cyst nematodes, Globodera rostochiensis and G. pallida. Ann Appl Biol 103:271–276
Bulman SR, Marshall JW (1997) Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR). N Z J Crop Hortic Sci 25:123–129
CAB International (2010) Globodera rostochiensis. Crop Protection Compendium, 2011 Edition. CAB International, Wallingford, UK
Dale MFB, De Scurrah MM (1998) Breeding for resistance to the potato cyst nematodes Globodera rostochiensis and Globodera pallida: strategies, mechanisms and genetic resources. In: Marks RJ, Brodie BB (eds) Potato cyst nematodes: biology, distribution and control. CABI, Wallingford, pp 167–195
Evans K (1993) New approaches for potato cyst nematode management. Nematropica 23:221–231
Evans K, Trudgill DL, Brown NJ (1977) Effects of potato cyst nematodes on potato plants. Vascular root system development in lightly and heavily infested susceptible and resistant varieties and its importance in nutrient and water uptake. Nematologica 23:153–164
Fenwick DW (1940) Methods for the recovery and counting of cysts of Heterodera schachtii from soil. J Helminthol 18:155–172
Finkers-Tomczak A, Bakker E, de Boer J, van der Vossen E, Achenbach U, Golas T, Suryaningrat S, Smant G, Bakker J, Goverse A (2011) Comparative sequence analysis of the potato cyst nematode resistance locus H1 reveals a major lack of co-linearity between three haplotypes in potato (Solanum tuberosum ssp.). Theor Appl Genet 122:595–608
Greco N (1993) Nematode problems affecting potato production in subtropical climates. Nematropica 23:213–220
Greco N, Moreno I (1992) Development of Globodera rostochiensis during three different growing seasons in Chile. Nematropica 22:175–181
Guy GL, Woodward J, Hinch JM (1992) Globodera rostochiensis and possibly G. pallida in Australia. J Nematol 24:594
Haverkort AJ, Trudgill DL (1995) Crop physiological responses to infection by potato cyst nematodes (Globodera spp). In: Haverkort AJ, MacKerron DKL (eds) Potato ecology and modelling of crops under conditions limiting growth. Current Issues in Production Ecology. Kluwer Academic Publishers, Dordrecht, pp 167–184
Hinch JM, Alberdi F, Smith SC, Woodward JR, Evans K (1998) Discrimination of European and Australian Globodera rostochiensis and G. pallida pathotypes by high performance capillary electrophoresis. Fundam Appl Nematol 21:123–128
Hodda M, Cook DC (2009) Economic impact from unrestricted spread of Potato Cyst Nematodes in Australia. Phytopathology 99:1387–1393
Kort J, Ross H, Rumpenhorst HJ, Stone AR (1977) An international scheme for identifying and classifying pathotypes of potato cyst-nematodes Globodera rostochiensis and Globodera pallida. Nematologica 23:333–339
La Mondia JA, Brodie BB (1986) Effects of initial nematode density on population dynamics of Globodera rostochiensis on resistant and susceptible potatoes. J Nematol 18:159–164
Quader M, Nambiar L, Cunnington J (2008) Conventional and real-time PCR-based species identification and diversity of potato cyst nematodes (Globodera spp.) from Victoria, Australia. Nematology 10:471–478
Sheridan JP, Miller AJ, Perry RN (2004) Early responses of resistant and susceptible potato roots during invasion by the potato cyst nematode Globodera rostochiensis. J Exp Bot 55:751–760
Stanton JM (1986) First record of potato cyst nematode Globodera rostochiensis in Australia. Australas Plant Pathol 15:87
Steinbach P (1972) Untersuchungen über das Verhalten von Larven des Kartoffelzystenälchens (Heterodera rostochiensis Wollenweber, 1923) an und in Wurzeln der Wirtspflanze Lycopersicon esculentum Mill. I. Zur Anlockwirkung von Tomatensämlingswurzeln gegenüber Kartoffelnematodenlarven. Biologisches Zentralblatt 91:493–500
Sullivan MJ, Inserra RN, Franco J, Moreno-Leheudé I, Greco N (2007) Potato cyst nematodes: plant host status and their regulatory impact. Nematropica 37:193–201
Toxopeus HJ, Huijsman CA (1953) Breeding for resistance to potato root eelworm. Euphytica 2:180–186
Trudgill DL (1980) Effects of Globodera rostochiensis and fertilisers on the mineral nutrient content and yield of potato plants. Nematologica 26:243–254
Trudgill DL (1991) Resistance to and tolerance of plant parasitic nematodes in plants. Annu Rev Phytopathol 29:167–192
Turner SJ (1998) Sample preparation, soil extraction and laboratory facilities for the detection of potato cyst nematodes. In: Marks RJ, Brodie BB (eds) Potato cyst nematodes: biology, distribution and control’. CABI, Wallingford, pp 75–90
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press Inc, London, pp 315–322
Whitehead AG, Turner SJ (1998) Management and regulatory control strategies for potato cyst nematodes (Globodera rostochiensis and Globodera pallida). In: Marks RJ, Brodie BB (eds) Potato cyst nematodes: biology, distribution and control. CABI, Wallingford, pp 135–152
Winslow RD (1954) Provisional lists of host plants of some root eelworms (Heterodera spp.). Ann Appl Biol 41:591–605
Wyss U (2002) Feeding behaviour of plant-parasitic nematodes. In: Lee DL (ed) The biology of nematodes. Taylor and Francis, London, pp 233–260
We would like to thank John Anderson, John Marshall (New Zealand Plant & Food Research) and Finlay Dale (Scottish Crops Research Institute) for discussions on the screening protocol; Pat Sharkey and David Eagling (Victorian Department of Primary Industries) for permission to develop a quarantine facility for the trial; Craig Murdoch (Victorian Department of Primary Industries) for access to PCN cysts; Lila Nambier and Motuil Quader (Victorian Department of Primary Industries) for assessment of cyst numbers and viability; Keith Blackmore (Victorian Certified Seed Authority) for access to data on cultivar planting area; and Phil Keane (La Trobe University) for plant pathology expertise. This work was funded by the Victorian Department of Primary Industries and Horticulture Australia Ltd.
This work was funded by the Victorian Department of Primary Industries and Horticulture Australia Ltd.
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Faggian, R., Powell, A. & Slater, A.T. Screening for resistance to potato cyst nematode in Australian potato cultivars and alternative solanaceous hosts. Australasian Plant Pathol. 41, 453–461 (2012). https://doi.org/10.1007/s13313-011-0098-y
- S. aviculare