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Hybridisation of Australian chickpea cultivars with wild Cicer spp. increases resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus)

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Abstract

Australian and international chickpea (Cicer arietinum) cultivars and germplasm accessions, and wild annual Cicer spp. in the primary and secondary gene pools, were assessed in glasshouse experiments for levels of resistance to the root-lesion nematodes Pratylenchus thornei and P. neglectus. Lines were grown in replicated experiments in pasteurised soil inoculated with a pure culture of either P. thornei or P. neglectus and the population density of the nematodes in the soil plus roots after 16 weeks growth was used as a measure of resistance. Combined statistical analyses of experiments (nine for P. thornei and four for P. neglectus) were conducted and genotypes were assessed using best linear unbiased predictions. Australian and international chickpea cultivars possessed a similar range of susceptibilities through to partial resistance. Wild relatives from both the primary (C. reticulatum and C. echinospermum) and secondary (C. bijugum) gene pools of chickpea were generally more resistant than commercial chickpea cultivars to either P. thornei or P. neglectus or both. Wild relatives of chickpea have probably evolved to have resistance to endemic root-lesion nematodes whereas modern chickpea cultivars constitute a narrower gene pool with respect to nematode resistance. Resistant accessions of C. reticulatum and C. echinospermum were crossed and topcrossed with desi chickpea cultivars and resistant F4 lines were obtained. Development of commercial cultivars with the high levels of resistance to P. thornei and P. neglectus in these hybrids will be most valuable for areas of the Australian grain region and other parts of the world where alternating chickpea and wheat crops are the preferred rotation.

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References

  • Abbo S, Berger J, Turner NC (2003) Evolution of cultivated chickpea: four bottlenecks limit diversity and constrain adaptation. Funct Plant Biol 30:1081–1087

    Article  Google Scholar 

  • Berger J, Abbo S, Turner NC (2003) Ecogeography of annual wild Cicer species: the poor state of the world collection. Crop Sci 43:1076–1090

    Article  Google Scholar 

  • Berger JD, Buck R, Henzell JM, Turner NC (2005) Evolution in the genus Cicer—vernalisation response and low temperature pod set in chickpea (C. arietinum L.) and its annual wild relatives. Aust J Agric Res 56:1191–1200

    Article  Google Scholar 

  • Berry DA (1987) Logarithmic transformations in ANOVA. Biometrics 43:439–456

    Article  PubMed  CAS  Google Scholar 

  • Bretag TW, MacLeod WJ, Kimber RBE, Moore KJ, Knights EJC, Davidson JA (2008) Management of Ascochyta blight in chickpeas in Australia. Australas Plant Pathol 37:486–497

    Article  CAS  Google Scholar 

  • Castillo P, Gomez-Barcina J-D (1996) Plant parasitic nematodes associated with chickpea in southern Spain and effect of soil temperature on reproduction of Pratylenchus thornei. Nematologica 42:211–219

    Article  Google Scholar 

  • Castillo P, Vovlas N (2007) Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, biology, pathogenicity and management. Nematology Monographs and Perspectives 6 (Series Editors DJ Hunt, RN Perry), 529 pp. Leden-Boston, Brill

    Google Scholar 

  • Castillo P, Jiménez-Díaz Gomez-Barcina A, Vovlas N (1995) Parasitism of the root-lesion nematode Pratylenchus thornei on chickpea. Plant Pathol 44:728–733

    Article  Google Scholar 

  • Castillo P, Vovlas N, Jiménez-Díaz RM (1998) Pathogenicity and histopathology of Pratylenchus thornei populations on selected chickpea genotypes. Plant Pathol 47:370–376

    Article  Google Scholar 

  • Collard BCY, Ades PK, Pang ECK, Brouwer JB, Taylor PWJ (2001) Prospecting for sources of resistance to Ascochyta blight in wild Cicer species. Australas Plant Pathol 30:271–276

    Article  Google Scholar 

  • Cook R, Evans K (1987) Resistance and tolerance. In: Brown RH, Kerry BR (eds) Principles and practice of nematode control in crops. Academic Press, New York, pp 179–231

    Google Scholar 

  • Cox HW, Kelly RM, Strong WM (2010) Pulse crops in rotation with cereals can be a profitable alternative to nitrogen fertiliser in central Queensland. Crop and Pasture Science 61:752–762

    Article  Google Scholar 

  • Croser JS, Ahmad F, Clarke HJ, Siddique KHM (2003) Utilisation of wild Cicer in chickpea improvement—progress, constraints and prospects. Aust J Agric Res 54:429–444

    Article  Google Scholar 

  • Dalal RC, Strong WM, Weston EJ, Cooper JE, Wildermuth GB, Lehane KJ, King AJ, Holmes CJ (1998) Sustaining productivity of a Vertisol at Warra, Queensalnd with fertilisers, no-tillage or legumes. 5. Wheat yields, nitrogen benefits and water-use efficiency of chickpea-wheat rotation. Aust J Exp Agric 38:489–501

    Article  Google Scholar 

  • Di Vito M, Greco N, Saxena MC (1992) Pathogenicity of Pratylenchus thornei on chickpea in Syria. Nematologia mediterranea 20:71–73

    Google Scholar 

  • Di Vito M, Zaccheo G, Catalano F (1995) Response of chickpea lines to Meloidogyne artiellia and Pratylenchus thornei. Supplement Nematologia mediterranea 23:81–83

    Google Scholar 

  • Di Vito M, Greco N, Singh KB, Saxena MC (1996) Sources of resistance to cyst nematode in cultivated and wild Cicer species. Genet Resour Crop Evol 43:103–107

    Article  Google Scholar 

  • Dropkin VH (1980) Introduction to plant nematology. Wiley, New York

    Google Scholar 

  • FAOSTAT (2011) http://faostat.fao.org/site/567/default.aspx#ancor

  • Felton WL, Marcellos H, Alston C, Martin RJ, Backhouse D, Burgess LW, Herridge DF (1998) Chickpea in wheat-based cropping systems of northern New South Wales. II. Influence on biomass, grain yield, and crown rot in the following wheat crop. Aust J Agric Res 49:401–407

    Article  CAS  Google Scholar 

  • Fortuner R (1977) Pratylenchus thornei. C.I.H. Description of Plant-parasitic Nematodes Set 7, No. 93. (Commonwealth Institute of Helminthology: St Albans, UK).

  • Gilmour AR, Cullis BR, Welham SJ, Thompson R (1999) ASREML Reference Manual. Biometric Bulletin No. 3. (NSW Agriculture: Orange, NSW, Australia).

  • Greco N (1987) Nematodes and their control in chickpea. In: Saxena MC, Singh KB (ed) The chickpea’. pp. 271–281. (CAB International: Wallingford, UK; The International Center for Agricultural Research in the Dry Areas: Syria).

  • Greco N, Di Vito M, Reddy MV, Saxena MC (1984) A preliminary report of survey of plant parasitic nematodes of leguminous crops in Syria. Nematologica mediterranea 12:87–93

    Google Scholar 

  • Greco N, Di Vito M, Saxena MC, Reddy MV (1988) Investigation on the root lesion nematode, Pratylenchus thornei, in Syria. Nematologica mediterranea 16:101–105

    Google Scholar 

  • Herridge DF, Marcellos H, Felton WL, Turner GL, Peoples MB (1995) Chickpea increases soil-N fertility in cereal systems through nitrate sparing and N2–fixation. Soil Biol Biochem 27:545–551

    Article  Google Scholar 

  • Hollaway GJ, Vanstone VA, Nobbs J, Smith JG, Brown JS (2008) Pathogenic nematodes of cereal crops in south-west Victoria, Australia. Australas Plant Pathol 37:505–510

    Article  Google Scholar 

  • Isbell RF (1996) The Australian soil classification. Revised edition. Melbourne, CSIRO Publishing

    Google Scholar 

  • Kelly AM, Smith AB, Eccleston JA, Cullis BR (2007) The accuracy of varietal selection using factor analytic models for multi-environment plant breeding trials. Crop Sci 47:1063–1070

    Article  Google Scholar 

  • Knights EJ, Brinsmead RB, Fordyce M, Wood JA, Kelly A, Harden S (2002). Use of the wild relative Cicer echinospermum in chickpea improvement. In: McComb JA (ed) Plant Breeding for the 11th Millenium. Proceedings of the 12th Australasian Plant Breeding Conference, Perth, Western Australia, 15–20 September 2002, 93–111

  • Knights EJ, Southwell RJ, Schwinghamer MW, Harden S (2008) Resistance to Phytophthora medicaginis Hansen and Maxwell in wild Cicer species and its use in breeding root rot resistant chickpea (Cicer arietinum L.). Aust J Agric Res 59:383–387

    Article  Google Scholar 

  • Muehlbauer FJ, Kaiser WJ, Simon CJ (1994) Potential for wild species in cool season food legume breeding. Euphytica 73:109–114

    Article  Google Scholar 

  • Pande S, Galloway J, Gaur PM, Siddique KNM, Tripathi HS, Taylor P, MacLeod MWJ, Basandrai AK, Bakr A, Joshi S, Krishna Kishore G, Isenegger DA, Narayana Rao J, Sharma M (2006) Botrytis grey mould of chickpea: a review of biology, epidemiology, and disease management. Aust J Agric Res 57:1137–1150

    Article  Google Scholar 

  • Pande S, Sharma M, Gaur PM, Tripathi S, Kaur L, Basandrai A, Khan T, Gowda CLL, Siddique KHM (2011) Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea. Australas Plant Pathol 40:149–156

    Article  Google Scholar 

  • Patterson HD, Thompson R (1971) Recovery of inter-block information when block sizes are unequal. Biometrika 58:545–554

    Article  Google Scholar 

  • Payne RW, Harding SA, Murray DA, Soutar DM, Baird DB, Welham SJ, Kane AF, Gilmour AR, Thompson R, Webster R, Tunnicliffe Wilson G (2004) GENSTAT for Windows, 8th edn. VSN International, Oxford

    Google Scholar 

  • Piepho H-P (1998) Empirical best linear unbiased prediction in cultivar trials using factor-analytic variance-covariance structures. Theor Appl Genet 97:195–201

    Article  Google Scholar 

  • Proctor JR, Marks CF (1974) The determination of normalising transformations for nematode count data from soil samples and of efficient sampling schemes. Nematologica 20:395–406

    Article  Google Scholar 

  • Redden RL, Berger JD (2007) History and origin of chickpea. In: Yadav SS, Redden R, Chen W, Sharma B (eds) Chickpea breeding and mangement. CABI, Wallingford, pp 1–13

    Chapter  Google Scholar 

  • Reen RA, Thompson JP (2009) A comparative study of methods for screening chickpea and wheat for resistance to root-lesion nematode Pratylenchus thornei. In Plant Health Management an Integrated Approach, 17th biennial Australasian Plant Pathology Society conference, Newcastle. (The Australasian Plant Pathology Society: Newcastle) p. 196

  • Riley IT, Kelly SJ (2002) Endoparasitic nematodes in cropping soils of Western Australia. Aust J Exp Agric 42:49–56

    Article  Google Scholar 

  • Riley IT, Wouts WM (2001) Pratylenchus and Radopholus species in agricultural soils and native vegetation in southern Australia. Trans R Soc S Aust 125:147–153

    Google Scholar 

  • Roberts PA (2002) Concepts and consequences of resistance. In: Starr JL, Cook R, Bridge J (eds) Plant resistance to parasitic nematodes. CABI Publishing, Wallingford, pp 23–41

    Chapter  Google Scholar 

  • Robinson GK (1991) That BLUP is a good thing: the estimation of random effects. Stat Sci 6:15–51

    Article  Google Scholar 

  • Sheedy JG, Thompson JP (2009) Resistance to the root-lesion nematode Pratylenchus thornei of Iranian landrace wheat. Australas Plant Pathol 38:478–489

    Article  Google Scholar 

  • Sikora RA, Greco N (1990) Nematode parasites of food legumes. Chapter 6. In: Luc M, Sikora RA, Bridge J (eds) Plant parasitic nematodes in subtropical and tropical agriculture, 181–235.CAB International

  • Singh KB, Ocampo B (1997) Exploitation of wild Cicer species for yield improvement in chickpea. Theor Appl Genet 95:418–423

    Article  Google Scholar 

  • Singh R, Sharma P, Varshney RK, Sharma SK, Singh NK (2008) Chickpea improvement: role of wild species and genetic markers. Biotechnol Genet Eng Rev 25:267–314

    Article  PubMed  CAS  Google Scholar 

  • Smith AB, Cullis BR, Thompson R (2001) Analysing variety by environment data using multiplicative mixed models and adjustments for spatial field trend. Biometrics 57:1138–1147

    Article  PubMed  CAS  Google Scholar 

  • Taylor SP, Hollaway GJ, Hunt CH (2000) Effect of field crops on population densities of Pratylenchus neglectus and P. thornei in southeastern Australia; Part 1 P. neglectus. J Nematol 32:591–599

    PubMed  CAS  Google Scholar 

  • Thompson JP (1990a) Treatments to eliminate root-lesion nematode (Pratylenchus thornei Sher and Allen) from a vertisol. Nematologica 36:123–127

    Article  Google Scholar 

  • Thompson JP (1990b) Soil sterilization methods to show VA-mycorrhizae aid P and Zn nutrition of wheat in vertisols. Soil Biol Biochem 22:229–240

    Article  CAS  Google Scholar 

  • Thompson CH, Beckmann GG (1959) Soils and land use in the Toowoomba Area, Darling Downs, Queensland. CSIRO Division of Soils: Melbourne. Soils and Land Use Series 28.

  • Thompson JP, Haak MI (1997) Resistance to root-lesion nematode (Pratylenchus thornei) in Aegilops tauschii Coss., the D-genome donor to wheat. Aust J Agric Res 48:553–559

    Article  Google Scholar 

  • Thompson JP, Brennan PS, Clewett TG, Sheedy JG, Seymour NP (1999) Progress in breeding wheat for tolerance and resistance to root-lesion nematode (Pratylenchus thornei). Australas Plant Pathol 28:45–52

    Article  Google Scholar 

  • Thompson JP, Greco N, Eastwood R, Sharma SB, Scurrah M (2000) Integrated control of nematodes of cool-season food legumes. pp 491–506. In: Knight R (ed) Linking Research and Marketing Opportunities for Pulses in the 21st Century. Proceedings Third International Food Legumes Research Conference, Kluwer Academic Publishers, Dordrecht

  • Thompson JP, Owen KJ, Stirling GR, Bell MJ (2008) Root-lesion nematodes (Pratylenchus thornei and P. neglectus): a review of recent progress in managing a significant pest of grain crops in northern Australia. Australas Plant Pathol 37:235–242

    Article  Google Scholar 

  • Thompson JP, Clewett TG, Sheedy JG, Reen RA, O’Reilly MM (2010) Occurrence of root-lesion nematodes (Pratylenchus thornei and P. neglectus) and stunt nematode (Merlinius brevidens) in the northern grain region of Australia. Australas Plant Pathol 39:254–264

    Article  Google Scholar 

  • Toker C (2009) A note on the evolution of kabuli chickpeas as shown by induced mutations in Cicer reticulatum Ladizinsky. Genet Resour Crop Evol 56:7–12

    Article  Google Scholar 

  • Trudgill DL (1992) Resistance to and tolerance of plant-parasitic nematodes in plants. Annu Rev Phytopathol 29:167–92

    Article  Google Scholar 

  • Vanstone VA, Hollaway GJ, Stirling GR (2008) Managing nematode pests in the southern and western regions of the Australian cereal industry: continuing progress in a challenging environment. Australas Plant Pathol 37:220–234

    Article  Google Scholar 

  • Whitehead AG, Hemming JR (1965) A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Ann Appl Biol 55:25–38

    Article  Google Scholar 

  • Yadav SS, Kumaat J, Yadav SK, Singh S, Yadav VS, Turner NC, Redden R (2006) Evaluation of Helicoverpa and drought resistance in desi and kabuli chickpea. Plant Genetic Resources: Characterization and Utilization 4:198–203

    Article  Google Scholar 

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Acknowledgements

This work was financially supported by the Grains Research and Development Corporation (GRDC) as part of the Australian Co-ordinated Chickpea Improvement Program (ACCIP). The following members of ACCIP kindly provided chickpea seed and/or comment on results: R. Brinsmead, M. English, J. Brouwer, C. Pittock, K. Hobson, T. Khan, W. Hawthorne, K. Siddique and T. Bretag. We also thank A. McIntyre, former Curator of the Australian Temperate Field Crops Collection, Horsham, Vic., for provision of seed of wild relatives of chickpea, and V. Vanstone for the culture of P. neglectus.

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  1. Beverley J. Gogel

    Present address: School of Agriculture Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia

Authors and Affiliations

  1. Agri-Science Queensland, Department of Employment, Economic Development and Innovation, Leslie Research Centre, PO Box 2282, Toowoomba, Qld, 4350, Australia

    John P. Thompson, Roslyn A. Reen, Timothy G. Clewett, Jason G. Sheedy & Alison M. Kelly

  2. Department of Employment, Economic Development and Innovation, Animal Research Institute, Yeerongpilly, Qld, 4105, Australia

    Beverley J. Gogel

  3. NSW Industry & Investment, Tamworth Agricultural Institute, 4 Marsden Park Road, Calala, NSW, 2340, Australia

    Edward J. Knights

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Thompson, J.P., Reen, R.A., Clewett, T.G. et al. Hybridisation of Australian chickpea cultivars with wild Cicer spp. increases resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus). Australasian Plant Pathol. 40, 601–611 (2011). https://doi.org/10.1007/s13313-011-0089-z

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