Abstract
Screening and identification of disease resistance to Botrytis grey mould (BGM, Botrytis cinerea) of chickpea is an important component to the development of integrated disease management strategies. The disease reaction to BGM with C. arietinum genotypes and C. bijugum accessions were assessed in growth chamber bioassays. The infection typically progressed with more severe necrosis and wilting from the shoot tip that could spread at least 2–4 internodes with the most susceptible reaction resulting in plant collapse and death. Fungal mycelium was commonly observed growing externally on shoot tips and sporulation occurred within 7–14 days. Initial screening to evaluate the disease progression from C. arietinum genotypes and C. bijugum accessions showed that disease symptoms were indicative of severity and increased with time. Moderate resistance was detected in four C. arietinum genotypes 99314–1003, FLIP97-529C, FLIP94-089C, FLIP94-508C and all six C. bijugum accessions. A detached leaf assay was effective in discriminating resistance in C. bijugum ILWC240 and susceptibility in FLIP97-90C and Lasseter genotypes, based on lesion number per leaflet. The detached leaf assay could be used for screening susceptible genotypes in less time than growth chamber bioassays. Resistance in C. bijugum indicated the potential use of wild Cicer species for novel sources of BGM resistance. Microscopic examination of the initial infection process with whole leaflet sections showed spore germination, appressoria formation as hyphal tip swelling and penetration directly through the cuticle, stomata and glandular stalks on the leaf surface. However, no remarkable differences between resistant and susceptible genotypes were shown within 72 h after inoculation (hai). This indicated that defence responses were likely to have been effective after 72 hai during the post-penetration or colonisation phase.
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References
Bouhassan A, Sadaki M, Tivoli B (2004) Evaluation of a collection of faba bean (Vicia faba L.) genotypes originating from the Maghreb for resistance to chocolate spot (Botrytis fabae) by assessment in the field and the laboratory. Euphytica 135:55–62
Clark CA, Lorbeer JW (1976) Comparative histopathology of Botrytis squamosa and B. cinerea on onion leaves. Phytopathology 66:1279–1289
Cole L, Dewey FM, Hawes CR (1996) Infection mechanisms of Botrytis species: pre-penetration and pre-infection processes of dry and wet conidia. Mycol Res 100:277–286
Collard BCY, Ades PK, Pang ECK, Brouwer JB, Taylor PWJ (2001) Prospecting for sources of resistance to ascochyta blight in wild Cicer species. Australas Plant Pathol 30:271–276
Cook DWM, Elmer PAG, Hill RA (2002) Quantitative inoculation with dry conidia of Botrytis cinerea. Australas Plant Pathol 31:217–222
DAFF (2011) Australian Food Statistics 2009–10. Australian Government Department of Agriculture, Fisheries and Forestry. http//:www.daff.gov.au
Davidson JA, Pande S, Bretag TW, Lindbeck KD, Krishna-Kishore G (2004) Biology and management of Botrytis spp. in legume crops. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: Biology, pathology and control. Kluwer, NL, pp 295–311
Dita MA, Rispail N, Prats E, Rubiales D, Singh KB (2006) Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes. Euphytica 147:1–24
Guimaraes RL, Chetelat RT, Stotz HU (2004) Resistance to Botrytis cinerea in Solanum lycopersicoides is dominant in hybrids with tomato and involves induced hyphal death. Eur J Plant Pathol 110:13–23
Hammer PE, Evensen KB (1994) Differences between rose cultivars in susceptibility to infection by Botrytis cinerea. Phytopathology 84:1305–1312
Holz G, Coertze S, Williamson B (2004) The ecology of Botrytis on plant surfaces. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: Biology, pathology and control. Kluwer, NL, pp 9–27
Isenegger DA, Ades PK, Ford R, Taylor PWJ (2008a) Status of the Botrytis cinerea species complex and microsatellite analysis of transposon types in South Asia and Australia. Fungal Divers 29:17–26
Isenegger DA, Macleod WJ, Ford R, Taylor PWJ (2008b) Genotypic diversity and migration of clonal lineages of Botrytis cinerea from chickpea fields of Bangladesh inferred by microsatellite markers. Plant Pathol 57:967–973
Kars L, van Kan JAL (2004) Extracellular enzymes and metabolites involved in pathogenesis of Botrytis. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: Biology, pathology and control. Kluwer, NL, pp 99–118
McDonald BA, Linde C (2002) The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica 124:163–180
Millan T, Clarke HJ, Siddique KHM, Buhariwalla HK, Gaur PM, Kumar J, Gil J, Kahl G, Winter P (2006) Chickpea molecular breeding: new tools and concepts. Euphytica 147:81–103
Nguyen TT, Taylor PWJ, Redden RJ, Ford R (2004) Genetic diversity estimates of Cicer using AFLP analysis. Plant Breeding 123:173–179
Nguyen TT, Taylor PWJ, Redden RJ, Ford R (2005) Resistance to Ascochyta rabiei (Pass.) Lab. in wild Cicer germplasm collection. Aust J Exp Agric 45:1291–1296
Pande S, Stevenson P, Narayana Rao J, Neupane RK, Chaudhary RN, Grzywacz D, Bourai VA, Krishna Kishore G (2005) Reviving chickpea production in Nepal through integrated crop management, with emphasis on Botrytis gray mold. Plant Dis 89:1252–1262
Pande S, Galloway G, Gaur PM, Siddique KHM, Tripathi HS, Taylor P, MacLeod MWJ, Basandrai BA, Joshi S, Krishna Kishore G, Isenegger DA, Narayana Rao J, Sharma M (2006) Botrytis grey mould of chickpea: A review of biology, epidemiology and disease management. Aust J Agric Res 57:1137–1150
Popelka JC, Terryn N, Higgins TJV (2004) Gene technology for grain legumes: can it contribute to the food challenge in developing countries? Plant Science 167:195–206
Prins TW, Tudzynski P, Von Tiedemann A, Tudzynski B, Ten Have A, Hansen ME, Tenberge K, Van Kan JAL (2000) Infection strategies of Botrytis cinerea and related necrotrophic pathogens. In: Kronstad JW (ed) Fungal pathology. Kluwer, NL, pp 33–64
Rewal N, Grewal JS (1989) Effect of temperature, light and relative humidity on conidial germination of three strains of Botrytis cinerea infecting chickpea. Indian Phytopathol 42:79–83
Stevenson PC, Haware MP (1999) Maackiain in Cicer bijugum Rech. F. associated with resistance to Botrytis grey mould. Biochem Syst Ecol 27:761–67
Stevenson PC, Veitch NC (1998) The distribution of isoflavonoids in Cicer. Phytochemistry 48:995–1001
Tivoli B, Baranger A, Avila CM, Banniza S, Barbetti M, Chen W, Davidson J, Lindbeck K, Kharrat M, Rubiales D, Sadiki M, Sillero JC, Sweetingham M, Muehlbauer FJ (2006) Screening techniques and sources of resistance to foliar diseases caused by major necrotrophic fungi in grain legumes. Euphytica 147:223–253
van Baarlen P, Legendre L, van Kan JAL (2004) Plant defence compounds against Botrytis infection. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Kluwer Academic Publishers, NL, pp 143–161
van Kan JAL (2006) Licensed to kill: the lifestyle of a necrotrophic plant pathogen. Trends Plant Sci 11:247–253
Verhoeff K (1980) The infection process and host-pathogen interactions. In: Coley-Smith JR, Verhoeff K, Jarvis WR (eds) The biology of botrytis. Academic, UK, pp 153–177
Acknowledgements
We extend our special thanks to Trevor Bretag (DPI-Horsham), Ted Knights (NSW Agriculture) and Kurt Lindbeck (NSW Agriculture) for the supply of germplasm and valuable discussions. We gratefully acknowledge funding from the Australian Centre for International Agricultural Research and The University of Melbourne.
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Isenegger, D.A., Ford, R. & Taylor, P.W.J. Disease reaction of chickpea (Cicer spp.) genotypes to Botrytis grey mould (Botrytis cinerea). Australasian Plant Pathol. 40, 583–590 (2011). https://doi.org/10.1007/s13313-011-0081-7
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DOI: https://doi.org/10.1007/s13313-011-0081-7