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Application of antagonistic rhizobacteria for control of Fusarium seedling blight and basal rot of lily

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Abstract

Three antagonistic bacteria, Streptomyces misionensis strain PMS101, Bacillus thermoglucosidasius strain PMB207, and S. sioyaensis strain PMS502, were tested for sensitivity to the foliar fungicide Sporgon (a.i. 50% prochloraz-Mn complex) and for efficacy in controlling Fusarium diseases of lily. Results showed that the growth of all three antagonistic strains of bacteria was completely suppressed by Sporgon at a concentration of 500 μg/mL, but B. thermoglucosidasius strain PMB207 and S. misionensis strain PMS101 were unaffected at concentrations of 100 μg/mL or lower. A large-scale trial in an automated and environment-controlled commercial greenhouse showed that treatment of scale bulblets of lily with Sporgon (100 μg /mL) and B. thermoglucosidasius strain PMB207 (1–1.2 × 107 cfu/mL) or 100 μg/mL Sporgon and S. misionensis strain PMS101 (1–1.4 × 107 cfu/mL) resulted in a significant reduction (P < 0.05) in the incidence of seedling blight caused by Fusarium proliferatum. The difference between these two treatments was not significant (P > 0.05). Results of the greenhouse and field experiments showed that treatment of scale bulblets or one-year-old bulbs of lily with B. thermoglucosidasius strain PMB207 (1–1.2 × 108 cfu/mL) or S. misionensis strain PMS101 (1–1.4 × 108 cfu/mL) without Sporgon was also effective in the control of basal rot caused by F. oxysporum f. sp. lilii. These studies reveal that B. thermoglucosidasius strain PMB207 and S. misionensis strain PMS101 are biocontrol agents which have potential for use in the commercial production of lily bulbs, as they can be used alone or in combination with the fungicide Sporgon at low concentration (<100 μg/mL).

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Acknowledgements

This research was funded by the Grant No. NSC95-2313-B-005-015 from the National Science and Technology Program for Agricultural Biotechnology, National Science Council, Taiwan. Dr. H. C. Huang is a Chair Professor at the Department of Plant Pathology, National Chung-Hsing University, Taichung, Taiwan.

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  1. Hung-Chang Huang

    Present address: Plant Pathology Division, Taiwan Agricultural Research Institute, Wufeng, Taichung, 41362, Taiwan

Authors and Affiliations

  1. Section of Propagation Technology, Seed Improvement and Propagation Station, Hsinshe, Taichung, 42642, Taiwan

    Wen-Chuan Chung

  2. Seed Testing Lab, Agriculture and Food Agency, Wufeng, Taichung, Taiwan

    Rey-Shung Wu

  3. Department of Plant Pathology, National Chung-Hsing University, Taichung, 40227, Taiwan

    Chia-Ping Hsu & Jenn-Wen Huang

  4. Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada, T1J 4B1 (Emeritus)

    Hung-Chang Huang

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  1. Wen-Chuan Chung
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Correspondence to Jenn-Wen Huang.

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Chung, WC., Wu, RS., Hsu, CP. et al. Application of antagonistic rhizobacteria for control of Fusarium seedling blight and basal rot of lily. Australasian Plant Pathol. 40, 269–276 (2011). https://doi.org/10.1007/s13313-011-0040-3

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