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Characterization of Exserohilum rostratum, a new causal agent of banana leaf spot disease in China

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Abstract

A new banana leaf spot disease (named as exserohilum leaf spot) caused by Exserohilum rostratum was found in Guangxi, China during a series of surveys on fungal species causing banana leaf diseases between 2005–2009. Three single spore derived isolates (CLER09, D087 and JL05) were obtained from diseased banana leaves and the pathogenicity of each isolate to banana plants was confirmed by inoculation tests based on Koch’s postulates. Three distinctive types of conidia (A, B and C) were produced by all isolates when subjected to a range of light environments. The isolates were identified as members of E. rostratum based on their morphological characters as well as rDNA-ITS (internal transcribed spacer) sequences. The effects of temperature and pH on vegetative growth and sporulation of the E. rostratum isolates were also characterized during this study. A similar host spectrum among the isolates was also observed in the host range tests on 128 plant species covering 47 families using a detached leaf inoculation technique.

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Acknowledgements

This research has been financed in part by grants from the Department of Science and Technology of Guangxi Zhuang Autonomous Region (Project No. 0728071), Science and Technology Bureau of Nanning City (Project No. 20060142B), Guangxi Academy of Agricultural Sciences (special research grant in 2008), and Nanyang Normal University (the projects for supporting the university science and technology innovation team of Henan province (No. 2010JRTSTHNO12) and for the construction of Henan province key discipline in biochemical and molecular biology).

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Authors and Affiliations

  1. College of Agriculture, Guangxi University, Nanning, 530005, People’s Republic of China

    Shan-Hai Lin, Qi-Qin Li & Gang Fu

  2. College of Life Sciences and Technology, Nanyang Normal University, Nanyang, 473061, People’s Republic of China

    Si-Liang Huang

  3. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, People’s Republic of China

    Chun-Jin Hu, Gang Fu, Li-Ping Qin, Ling Xie, Zhen-Lu Cen & Wei-Hong Yan

  4. Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, People’s Republic of China

    Yue-Feng Ma

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  1. Shan-Hai Lin
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Correspondence to Si-Liang Huang.

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Lin, SH., Huang, SL., Li, QQ. et al. Characterization of Exserohilum rostratum, a new causal agent of banana leaf spot disease in China. Australasian Plant Pathol. 40, 246–259 (2011). https://doi.org/10.1007/s13313-011-0037-y

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