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Survey of Botryosphaeriaceae associated with grapevine decline in the Hunter Valley and Mudgee grape growing regions of New South Wales

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Species belonging to the fungal family Botryosphaeriaceae are important pathogens of grapevines in Australia. A survey of declining grapevines in the Hunter Valley and Mudgee grape growing regions of New South Wales revealed 36% were infected with species belonging to the Botryosphaeriaceae. The incidence of Diplodia seriata was greatest, followed by Neofusicoccum parvum, Botryosphaeria dothidea and Lasiodiplodia theobromae. These identifications were made using a combination of molecular and morphological characters. Although D. seriata was the most common species found, its role as a primary pathogen of grapevines in Australia is yet to be ascertained. The accuracy of estimating the incidence of species was increased by surveying up to 25 grapevines per vineyard and by sampling both the trunks and cordons. Other pathogens capable of causing trunk diseases were also isolated in this survey, demonstrating that diagnosis based on symptoms alone is not sufficient and isolations on artificial media and sometimes DNA sequencing are required for a definitive diagnosis of the causal organism of decline.

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  • Auger J, Esterio M, Ricke G, Perez I (2004) Black dead arm and basal canker of Vitis vinifera cv. Red Globe caused by Botryosphaeria obtusa in Chile Plant Disease 88:1286

    Google Scholar 

  • Burgess T, Wingfield BD, Wingfield MJ (2001) Comparison of genotypic diversity in native and introduced populations of Sphaeropsis sapinea isolated from Pinus radiata. Mycological Research 105:1331–1339

    Article  Google Scholar 

  • Burgess T, Sakalidis M, Hardy G (2006) Gene flow of the canker pathogen Botryosphaeria australis between Eucalyptus globulus plantations and native eucalypt forests in Western Australia. Austral Ecology 31:559–566

    Article  Google Scholar 

  • Castillo-Pando M, Somers A, Green CD, Priest M, Sriskanthades M (2001) Fungi associated with dieback of Semillon grapevines in the Hunter Valley of New South Wales. Australasian Plant Pathology 30:59–63

    Article  Google Scholar 

  • Crous PW, Slippers B, Wingfield MJ, Rheeder J, Marasas WFO, Phillips AJL, Alves A, Burgess T, Barber P, Groenewald JZ (2006) Phylogenetic lineages in the Botryosphaeriaceae. Studies in Mycology 55:235–253

    Article  PubMed  Google Scholar 

  • Cunnington JH, Priest MJ, Powny RA (2007) Diversity of Botryosphaeria species on horticultural plants in Victoria and New South Wales. Australasian Plant Pathology 36:157–159

    Article  Google Scholar 

  • Denman S, Crous PW, Taylor JE, Kang J-C, Pascoe I, Wingfield MJ (2000) An overview of the taxonomic history of Botryosphaeria, and a re-evaluation of its anamorphs based on morphology and ITS rDNA phylogeny. Studies in Mycology 45:129–140

    Google Scholar 

  • Eck RV, Dayhoff MO (1966) Atlas of Protein Sequence and Structure. National Biomedical Research Foundation, Silver Springs, Maryland

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:78–791

    Article  Google Scholar 

  • Glastone J (1992) Viticulture and environment. Winetitles, South Australia, Australia

    Google Scholar 

  • Larignon P, Fulchic R, Cere L, Dubos B (2001) Observation on black dead arm in French vineyards. Phytopathologia Mediterranea 40:S336–S342

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95–98

    CAS  Google Scholar 

  • Hewitt RD (1988) Diplodia cane dieback and bunch rot. In ‘Compendium of grape diseases’. (Eds RC Pearson, AC Goheen). pp. 25–26 (APS Press, The American Phytopathological Society: Minnesota, USA)

  • Huang X (1991) A contig assembly program based on sensitive detection of fragment overlaps. Genomics 14:18–25

    Article  CAS  Google Scholar 

  • Nei M, Kumar S (2000) Molecular evolution and phylogenetics. (Oxford University Press: New York, USA)

  • Pennycook SR, Samuels GJ (1985) Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (Kiwifruit) in New Zealand. Mycotaxon 24:445–458

    Google Scholar 

  • Phillips AJL (1998) Botryosphaeria dothidea and other fungi associated with excoriose and dieback of grapevines in Portugal. Journal of Phytopathology 146:327–332

    Article  Google Scholar 

  • Phillips AJL (2002) Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathologia Mediterranea 41:3–18

    Google Scholar 

  • Phillips AJL (2007) Key to the various lineages in “Botryosphaeria”. Version 01 saved 8 October 2007. Centro de Recursos Microbiológicos, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829–516 Caparica, Portugal.

  • Phillips AJL, Alves A, Pennycook SR, Johnston PR, Ramaley A, Akulov A, Crous PW (2008) Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryophaeriaceae. Persoonia 21:29–55

    CAS  PubMed  Google Scholar 

  • Pitt W, Huang R, Savocchia S, Steel CC (2008) First report of Dothiorella iberica (‘Botryosphaeriaiberica) associated with grapevine decline in Australia. In ‘Proceedings of the 6th International Workshop on Grapevine Trunk Diseases’, (Florence: Italy, 1–3 September).

  • Pitt W, Huang R, Steel CC, Savocchia S (2010) Identification, distribution and current taxonomy of Botryosphaeriaceae species associated with grapevine decline in New South Wales and South Australia. Australian Journal of Grape and Wine Research 16:258–271

    Article  Google Scholar 

  • Qiu Y, Savocchia S, Steel CC, Ash G (2008) Botryosphaeria dothidea associated with grapevine trunk disease in south-eastern Australia. Australasian Plant Pathology 37:482–485

    Article  Google Scholar 

  • Rayner R (1970) A mycological colour chart. (CMI and British Mycological Society: Kew, Surrey: UK)

  • Savocchia S, Steel CC, Stodart BJ, Somers A (2007) Pathogenicity of Botryosphaeria species isolated from declining grapevines in sub-tropical regions of eastern Australia. Vitis 46:27–32

    Google Scholar 

  • Slippers B, Crous PW, Denman S, Coutinho TA, Wingfield BD, Wingfield MJ (2004a) Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea. Mycologia 96:83–101

    Article  CAS  Google Scholar 

  • Slippers B, Fourie G, Crous PW, Coutinho TA, Wingfield BD, Wingfield MJ (2004b) Multiple gene sequences delimit Botryosphaeria australis sp. nov. from B. lutea. Mycologia 96:1028–1039

    Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Taylor A, Hardy GES, Wood P, Burgess T (2005) Identification and pathogenicity of Botryosphaeria species associated with grapevine decline in Western Australia. Australasian Plant Pathology 34:187–195

    Article  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680

    Article  CAS  PubMed  Google Scholar 

  • Urbez-Torres JR, Leavitt GM, Voegel TM, Gubler WD (2006) Identification and distribution of Botryosphaeria spp. associated with grapevine cankers in California. Plant Disease 90:1490–1503

    Article  CAS  Google Scholar 

  • Urbez-Torres JR, Luque J, Gubler WD (2007) First report of Botryosphaeria iberica and B. viticola associated with grapevine decline in California. Plant Disease 91:772

    Article  Google Scholar 

  • Urbez-Torres JR, Gubler WD (2009) Pathogenicity of Botryosphaeriaceae species isolated from grapevine cankers in California. Plant Disease 93:584–592

    Article  Google Scholar 

  • van Niekerk JM, Crous PW, Groenewald JZ, Fourie PH, Halleen F (2004) DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines. Mycologia 96:781–798

    Article  Google Scholar 

  • van Niekerk JM, Fourie PH, Halleen F, Crous PW (2006) Botryosphaeria spp. as grapevine trunk disease pathogens. Phytopathologia Mediterranea 45:S43–S54

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In ‘PCR protocols: a guide to methods and applications’. (Eds MA Innis, DH Gelfand, JJ Sninsky, TJ White). pp. 315–322. (Academic Press: San Diego, California)

  • Witcher W, Clayton CN (1963) Blueberry stem blight caused by Botryosphaeria dothidea (B. ribis). Phytopathology 42:521–525

    Google Scholar 

  • Wunderlich N, Steel CC, Ash G, Raman H, Savocchia S (2008) Identification of Botryosphaeria spp. and first report of Dothiorella viticola (‘Botryosphaeriaviticola) associated with bunch rot in Australia. In ‘Proceedings of the 6th International Workshop on Grapevine Trunk Diseases’, (Florence: Italy, 1–3 September)

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This research was supported by the Australian Research Council's Linkage Projects funding scheme with Bayer CropScience, the Hunter Valley Vineyard and Mudgee Wine Grape Growers Associations as industry partners. Thanks to Craig Poynter of the Spatial Data Analysis Network (SPAN), Charles Sturt University for assistance with Fig. 1.

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Correspondence to Y. Qiu.

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Qiu, Y., Steel, C.C., Ash, G.J. et al. Survey of Botryosphaeriaceae associated with grapevine decline in the Hunter Valley and Mudgee grape growing regions of New South Wales. Australasian Plant Pathol. 40, 1–11 (2011).

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