Abstract
Objective
To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM).
Method
The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and results were compared.
Result
Among 24 neonates, the pathogens Identified included E. coli K1, GBS, Streptococcus pneumoniae and Listeria. PCR identified 20 (83.3%) pathogens, and culture 4 (16.7%) pathogens. Prior antibiotics were administered to 20 (83.3%) neonates in whom PCR identified 17 (85%) and culture 3 (15%) pathogens.
Conclusion
CSF PCR had a higher yield of pathogens than CSF culture in confirmed neonatal bacterial meningitis with a high rate of prior antibiotic therapy.
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TQNN,AS,HN: conceptualized and designed the study; TQNN,TVN,MDT: collected the data and performed laboratory analyses; HN,TQNN: analyzed the data and composed the first manuscript. The document was critically reviewed and approved all authors who are accountable for the totality of the work.
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IEC, National Children’s Hospital, Hanoi; No. 1565/BVNTW-VNCSKTE.
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Nguyen, T.Q.N., Van Nguyen, T., Tran, M.D. et al. Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam. Indian Pediatr 59, 943–945 (2022). https://doi.org/10.1007/s13312-022-2669-0
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DOI: https://doi.org/10.1007/s13312-022-2669-0