Abstract
Objectives
To estimate the regional cutoff of optical density (OD) values for immunoglobulin M (IgM) antibodies against Orientia tsutsugamushi in serum and cerebrospinal fluid (CSF) for clinical diagnosis of scrub typhus and immunoglobulin G (IgG) antibodies in serum for sero-epidemiology in Gorakhpur, Uttar Pradesh, India.
Methods
We used data from a serological investigation of acute encephalitis syndrome patients (n=407) during the 2016 outbreak in Gorakhpur, India to determine the cutoff for OD values for IgM antibodies, and from community-based serosurveys (n=1991) to estimate the cutoff for OD values for IgG antibodies.
Results
We determined regionally relevant cutoff for OD values of 0.76 for IgM antibodies in serum and 0.22 in cerebrospinal fluid for scrub typhus diagnosis. For serosurveys, IgG antibody cutoff was 1.5.
Conclusions
We have proposed locally relevant cutoffs for scrub typhus endemic regions, which may be useful for correctly classifying infected population.
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Acknowledgement
Dr Rashmi Arora, Former Head, Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi for her support and technical inputs on the study.
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Contributors
MDG: conceived the study; MDG, SK, MM: designed the study protocol and were involved in sample/data collection; AM, SS, VB, JD: carried out laboratory investigations; MG, MDG: analysed the data; MDG, MG, DG, MM: inter-preted these data; MG, MDG: drafted the manuscript; DG, MM: critically revised the manuscript for intellectual content. All authors read and approved the final manuscript, and agree to be accountable for; all aspects of the manuscript. Ethical clearance: The institutional ethics committee of National AIDS Research Institute, Pune; No. NARI EC/2015-24 dated 13 August, 2015 and NARI EC/2016-15 dated 12 September, 2015. National Institute of Epidemiology, Chennai; No.NIE/IHEC/201507/-01 and dated 20 July, 2016.
Funding
Indian Council of Medical Research, New Delhi
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None stated.
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Gupte, M.D., Gupte, M., Kamble, S. et al. Detection of Immunoglobulin M and Immunoglobulin G Antibodies Against Orientia tsutsugamushi for Scrub Typhus Diagnosis and Serosurvey in Endemic Regions. Indian Pediatr 57, 1131–1134 (2020). https://doi.org/10.1007/s13312-020-2067-4
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DOI: https://doi.org/10.1007/s13312-020-2067-4