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Endotracheal aspirate microscopy, cultures and endotracheal tube tip cultures for early prediction of ventilator associated pneumonia in neonates

Indian Pediatrics volume 54, pages 211–214 (2017)Cite this article

Abstract

Objective

To evaluate the utility of endotracheal aspirate microscopy, culture and endotracheal tube tip culture for early diagnosis of ventilator-associated pneumonia in neonates.

Methods

Inborn ventilated neonates were followed-up for ventilator-associated pneumonia using Center for Disease Control and Prevention (CDC) criteria. Endotracheal aspirate microscopy, culture and endotracheal tube tip cultures were performed.

Results

Ventilator-associated pneumonia occurred in 28/68 (41%) neonates as per CDC criteria. Endotracheal aspirate microscopy (≥5 polymorphonuclear cells per high power field) and endotracheal aspirate culture had 78.6% and 75% sensitivity, 87.5% and 90% specificity, positive predictive value of 81.5% and 84%, and negative predictive value of 85.4% and 83.72%, respectively. Mean (SD) time of result of microscopy and endotracheal aspirate culture was 55.7 (4.3) h and 108.3 (19.7) h, respectively in comparison to diagnosis made at 143.5 (23.3) h, as per CDC criteria.

Conclusion

Endotracheal aspirate microscopic examination and culture can be supportive in objective diagnosis of ventilator-associated pneumonia with an added advantage of earlier prediction.

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Author information

Affiliations

  1. Department of Neonatology, Lokmanya Tilak Municipal Medical College and General Hospital, Sion, Mumbai, 400 022, India

    Mahendra Kumar Gupta, Jayashree Mondkar, Deepraj Hegde & Sorabh Goel

  2. Department of Microbiology, Lokmanya Tilak Municipal Medical College and General Hospital, Sion. Mumbai, India

    Anjali Swami

Authors
  1. Mahendra Kumar Gupta
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  2. Jayashree Mondkar
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  3. Anjali Swami
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  4. Deepraj Hegde
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  5. Sorabh Goel
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Corresponding author

Correspondence to Mahendra Kumar Gupta.

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Gupta, M.K., Mondkar, J., Swami, A. et al. Endotracheal aspirate microscopy, cultures and endotracheal tube tip cultures for early prediction of ventilator associated pneumonia in neonates. Indian Pediatr 54, 211–214 (2017). https://doi.org/10.1007/s13312-017-1033-2

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  • DOI: https://doi.org/10.1007/s13312-017-1033-2

Keywords

  • Complications
  • Diagnosis
  • Intensive care
  • Nosocomial sepsis