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Rotavirus infections in children vaccinated against rotavirus in Pune, Western India

Indian Pediatrics volume 53, pages 589–593 (2016)Cite this article

Abstract

Objective

To characterize rotavirus infections detected in rotavirus vaccinated children hospitalized for acute gastroenteritis.

Design

Observational, hospital-based study.

Setting

Three hospitals in Pune, Western India.

Participants

Children aged <5 years hospitalized for acute gastroenteritis during 2013-14.

Methods

Rotavirus capture ELISA was performed on all stool samples that were collected from patients following informed consent from parents. VP7 and VP4 genes of rotavirus strains were genotyped by multiplex RT-PCR. Stool samples from vaccinated children were tested for other enteric viruses.

Results

Among the 529 children, 53 were vaccinated with at least one dose of the rotavirus vaccine. There was no difference in the mean (SD) (months) age of vaccinated [14.8 (10.6)] and unvaccinated [14.4 (10.5)] children. Rotavirus positivity was significantly higher (47%) in unvaccinated than in vaccinated (28.3%) children (P=0.01). Mean Vesikari score and severe cases were significantly more in rotavirus positive than in negative children within unvaccinated group (P<0.001), while these did not differ within the vaccinated group. Rotavirus strain G1P[8] was identified as the most prevalent strain in both, vaccinated (60%) and unvaccinated (72.8%) groups. No association was found between mean Vesikari score and viral coinfections.

Conclusions

This study suggests decline in rotavirus positivity in rotavirus-vaccinated children hospitalized for acute gastroenteritis and high prevalence of G1P[8] and non-rotaviral co-infections in Pune, Western India.

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Author information

Affiliations

  1. Enteric Viruses Group, National Institute of Virology, Pune, India

    Preeti Jain, Gopalkrishna Varanasi, Rohan Ghuge & Shobha Chitambar

  2. Bharati Vidyapeeth Medical College and Hospital, Pune, India

    Vijay Kalrao

  3. Sant Dnyaneshwar Medical Foundation & Research Centre’s Shaishav Clinic, Pune, India

    Ram Dhongade

  4. King Edward Memorial Hospital, Pune, India

    Ashish Bavdekar

  5. National Institute of Epidemiology, Chennai, India

    Sanjay Mehendale

  6. Enteric Viruses Group, National Institute of Virology, 20-A, Ambedkar Road, PO Box No 11, Pune, 411 001, India

    Shobha Chitambar

Authors
  1. Preeti Jain
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  2. Gopalkrishna Varanasi
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  3. Rohan Ghuge
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  4. Vijay Kalrao
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  5. Ram Dhongade
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  6. Ashish Bavdekar
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  7. Sanjay Mehendale
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  8. Shobha Chitambar
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Corresponding author

Correspondence to Shobha Chitambar.

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Jain, P., Varanasi, G., Ghuge, R. et al. Rotavirus infections in children vaccinated against rotavirus in Pune, Western India. Indian Pediatr 53, 589–593 (2016). https://doi.org/10.1007/s13312-016-0893-1

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  • DOI: https://doi.org/10.1007/s13312-016-0893-1

Keywords

  • Epidemiology
  • Diarrhea
  • Rotavirus vaccine