Skip to main content

Advertisement

SpringerLink
Log in

Is Xpert MTB/RIF assay in gastric lavage aspirate useful for diagnosis of smear-negative childhood pulmonary tuberculosis?

  • Journal Club
  • Published:
Indian Pediatrics Aims and scope Submit manuscript

Summary

This study evaluated the Xpert MTB/RIF assay for diagnosis of smear-negative childhood pulmonary tuberculosis (TB) using gastric lavage aspirates (GLA) in 211 Chinese children. The sensitivity in detecting children with a clinical diagnosis of TB for MGIT and Xpert was 12.1% (95% CI 9.3%, 14.9%) and 48.6% (95% CI 44.4%, 52.8%), respectively. The authors concluded that Xpert MTB/RIF assay is an excellent tool for the diagnosis of smear-negative childhood with GLA samples. The high proportion of very low mycobacterial load in the GLA samples from smear-negative TB cases may increase the frequency for obtaining indeterminate RIF resistance results by Xpert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. No authors listed. Cepheid Receives FDA Market Authorization for Xpert MTB/RIF. Available from: http://ir.cepheid.com/releasedetail.cfm?releaseid=780520. Accessed November 8, 2014.

  2. World Health Organization. Automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF assay for the diagnosis of pulmonary and extra-pulmonary TB in adults and children. Policy update. Available from: http://apps.who.int/iris/bitstream/10665/112472/1/9789241506335_eng.pdf. Accessed November 5, 2014

  3. World Health Organization. Guidance for national tuberculosis programmes on the management of tuberculosis in children Second edition Available from: http://apps.who.int/medicinedocs/documents/s21535en/s21535en.pdf. Accessed November 10, 2014

  4. Steingart KR, Schiller I, Horne DJ, Pai M, Boehme CC, Dendukuri N. Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane Database Syst Rev 2014;1:CD009593.

    PubMed  Google Scholar 

  5. Government of India. Central TB Division Directorate General of Health Services, Ministry of Health and Family Welfare. TB India 2014 Revised National TB Control Programme. Annual Status Report. Available from: http://www.tbcindia.nic.in/pdfs/TB%20INDIA%202014.pdf. Accessed November 10, 2014.

  6. Kumar A, Gupta D, Nagaraja SB, Singh V, Sethi GR, Prasad J; Indian Academy of Pediatrics. Updated national guidelines for pediatric tuberculosis in India, 2012. Indian Pediatr. 2013;50:301–6.

    Article  PubMed  Google Scholar 

  7. Pang Y, Wang Y, Zhao S, Liu J, Zhao Y, Li H. Evaluation of the Xpert MTB/RIF assay in gastric lavage aspirates for diagnosis of smear-negative childhood pulmonary tuberculosis. Pediatr Infect Dis J. 2014;33:1047–51.

    Article  PubMed  Google Scholar 

  8. No authors listed. Critical Appraisal Skills Programme (CASP) Diagnostic Test checklist_14.10.10. Available from: http://www.caspinternational.org/mod_product/uploads/CASP_Diagnostic_Checklist_14.10.10.pdf. Accessed October 10, 2014.

  9. No authors listed. Critical Appraisal Skills Programme (CASP) Diagnostic Test checklist_14.10.10. Available from: http://www.caspinternational.org/mod_product/uploads/CASP_Diagnostic_Checklist_14.10.10.pdf. Accessed October 10, 2014.

  10. Nhu NT, Ha DT, Anh ND, Thu DD, Duong TN, Quang ND, et al. Evaluation of Xpert MTB/RIF and MODS assay for the diagnosis of pediatric tuberculosis. BMC Infect Dis. 2013;23;13:31.

    Article  Google Scholar 

  11. Tortoli E, Russo C, Piersimoni C, Mazzola E, Dal Monte P, Pascarella M, et al. Clinical validation of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis. Eur Respir J. 2012;40:442–7.

    Article  PubMed  Google Scholar 

  12. Kabra SK, Lodha R, Seth V. Some current concepts on childhood tuberculosis. Indian J Med Res 2004;120:387–97.

    CAS  PubMed  Google Scholar 

  13. World Health Organization, 2013. WHO Global Tuberculosis Control Report: Geneva, Switzerland. http://www.who.int/tb/publications/factsheet_global.pdf . Accessed October 16, 2014.

  14. Swaminathan S, Rekha B. Pediatric tuberculosis: Global overview and challenges. Clin Infect Dis. 2010;50:S184–94.

    Article  Google Scholar 

  15. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, et al. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010;363:1005–15.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Bates M, O’Grady J, Maeurer M, Tembo J, Chilukutu L, Chabala C, et al.. Assessment of the Xpert MTB/RIF assay for diagnosis of tuberculosis with gastric lavage aspirates in children in sub-Saharan Africa: A prospective descriptive study. Lancet Infect Dis. 2013;13:36–42.

    Article  PubMed  Google Scholar 

  17. Parashar D, Kabra SK, Lodha R, Singh V, Mukherjee A, Arya T, et al. Does neutralization of gastric aspirates from children with suspected intrathoracic tuberculosis affect mycobacterial yields on MGIT culture? J Clin Microbiol. 2013; 51:1753–6.

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, PGIMER, Chandigarh, India

    Joseph L. Mathew

  2. Madras Medical College, Chennai, India

    D. Vijayasekharan

  3. Department of Laboratory Medicine, AIIMS, New Delhi, India

    Sarman Singh

Authors
  1. Joseph L. Mathew
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Vijayasekharan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sarman Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Joseph L. Mathew, D. Vijayasekharan or Sarman Singh.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mathew, J.L., Vijayasekharan, D. & Singh, S. Is Xpert MTB/RIF assay in gastric lavage aspirate useful for diagnosis of smear-negative childhood pulmonary tuberculosis?. Indian Pediatr 51, 1007–1011 (2014). https://doi.org/10.1007/s13312-014-0548-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13312-014-0548-z

Keywords

Search

Navigation