Immunotherapy Prospects for Painful Small-fiber Sensory Neuropathies and Ganglionopathies
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The best-known peripheral neuropathies are those affecting the large, myelinated motor and sensory fibers. These have well-established immunological causes and therapies. Far less is known about the somatic and autonomic “small fibers”; the unmyelinated C-fibers, thinly myelinated A-deltas, and postganglionic sympathetics. The small fibers sense pain and itch, innervate internal organs and tissues, and modulate the inflammatory and immune responses. Symptoms of small-fiber neuropathy include chronic pain and itch, sensory impairment, edema, and skin color, temperature, and sweating changes. Small-fiber polyneuropathy (SFPN) also causes cardiovascular, gastrointestinal, and urological symptoms, the neurologic origin of which often remains unrecognized. Routine electrodiagnostic study does not detect SFPN, so skin biopsies immunolabeled to reveal axons are recommended for diagnostic confirmation. Preliminary evidence suggests that dysimmunity causes some cases of small-fiber neuropathy. Several autoimmune diseases, including Sjögren and celiac, are associated with painful small-fiber ganglionopathy and distal axonopathy, and some patients with “idiopathic” SFPN have evidence of organ-specific dysimmunity, including serological markers. Dysimmune SFPN first came into focus in children and teenagers as they lack other risk factors, for example diabetes or toxic exposures. In them, the rudimentary evidence suggests humoral rather than cellular mechanisms and complement consumption. Preliminary evidence supports efficacy of corticosteroids and immunoglobulins in carefully selected children and adult patients. This paper reviews the evidence of immune causality and the limited data regarding immunotherapy for small-fiber-predominant ganglionitis, regional neuropathy (complex regional pain syndrome), and distal SFPN. These demonstrate the need to develop case definitions and outcome metrics to improve diagnosis, enable prospective trials, and dissect the mechanisms of small-fiber neuropathy.
KeywordsGanglionitis Complex regional pain syndrome Polyneuropathy C-fiber Neuropathic pain Small-fiber neuropathy
Peripheral neurons used to be neatly categorized into motor, sensory, or autonomic. Sensory neurons were divided into large myelinated low-threshold receptors versus small-diameter high-threshold receptors for painful stimuli (nociception). Then molecular study blurred the distinction between nociceptive and autonomic axons. The culprit is the small-diameter unmyelinated C-fiber, which does far more than sense pain. This evolutionarily primitive neuron functions bidirectionally—its distal axon terminal not only transduces and transmits pain, but it also releases electrical and paracrine chemical signals that maintain tissue homeostasis. C-fiber trophic signals are critical for the integrity of skin and bone for instance [1, 2]. Other C-fiber paracrine secretions modulate tissue responses to injury or threat. In “neurogenic inflammation” C-fibers release calcitonin-gene related peptide (CGRP) and substance P neuropeptides from their distal axon terminals. These trigger vasodilation, plasma protein extravasation, tissue edema, hemoconcentration, and leukocyte diapedesis . C-fibers also modulate immune responses, with mast cells in close physical and chemical contact . In turn, C-fiber signaling is modified by inflammatory signals, including serotonin, histamine, glutamate, adenosine triphosphate, adenosine, substance P, calcitonin-gene related peptide, bradykinin, eicosanoids prostaglandins, thromboxanes, leukotrienes, endocannabinoids, nerve growth factor, inflammatory cytokines, and protons. These bind to C-fiber surface receptors, including G protein-coupled receptors, transient receptor potential channels, acid-sensitive ion channels, 2-pore potassium channels, and receptor tyrosine kinases, as reviewed in Basbaum et al. . These allow injury and inflammation to trigger and sensitize pain signaling. Crosstalk between nociceptive and immune cells contributes to host defense and immune-mediated diseases , and there is preliminary evidence that disturbed immunity underlies some cases of painful small-fiber neuropathy (SFN). This suggests the novel possibility that immunotherapy might be a more effective treatment for pain (and other symptoms) than pain relievers in such patients. Given the lackluster long-term efficacy, potentially disastrous complications of chronic opioid therapy, and its lack of disease-modifying activity, this hypothesis deserves further consideration.
Immune-mediated Painful Sensory Ganglionitis/Neuronitis
There is no consensus on how best to treat Sjögren syndrome (SS). Some patients receive no immunotherapy, and among those treated, there is no standard regime [16, 17]. Common immunotherapies include hydroxychloroquine low-dose glucocorticoids (<20 mg/day), and high-dose glucocorticoids (>20 mg/day). Second-tier options include rituximab (RTX) and intravenous immunoglobulins (IVIg) . Their relative benefit for neuropathy is unknown, as most randomized clinical trials (RCT) for SS did not include neurological end points. A recent RCT reported no benefit of hydroxychloroquine 400 mg/d or RTX (1 g given twice) for pain or other outcomes [20, 21]. Small case series support the use of IVIg for ataxic (large-fiber) sensory ganglionitis . The best evidence comes from a French multicenter study documenting efficacy of IVIg 2 g/kg for SS neuropathy, including nonataxic (small-fiber) ganglionitis .
Another immunological cause of ganglionitis is paraneoplastic autoimmunity associated with antibodies to Hu or to the collapsin response mediator protein-5 (CRMP-5) [24, 25]. Small-cell lung tumors are particular triggers because of their neuroendocrine origin. The key therapy is tumor ablation, which removes antigenic drivers and effects cure, but while searching for the tumor, or if no tumor is found, immunotherapy is mandatory. Sensory ganglionitis has not been a primary outcome for clinical trials; thus, agents with documented efficacy against other neurological symptoms are used [26, 27], usually beginning with corticosteroids and IVIg . One tumorless patient with anti-Hu sensory neuronopathy and gastric dysmotility achieved sustained improvement for 2 years after RTX treatment . Assessing the outcomes of immunotherapy treatment for ganglionitis is complicated by the fact that stopping the immune attack may not improve symptoms caused by earlier death of neurons. For instance, postherpetic neuralgia can persist long after the shingles ganglionitis resolves . However, usually it gradually improves and often resolves, presumably reflecting sprouting of axons from surviving cell bodies into deafferented targets.
Complex Regional Pain Syndrome
The pathogenesis of this historically rich post-traumatic neuropathic pain syndrome is still incompletely understood. One core feature is painful mono- or oligo- post-traumatic neuropathy in 1 extremity . Ninety percent of cases are triggered by trauma (sprains/strains, fractures, iatrogenic), and some of the nontraumatic cases follow known or suspected focal nerve lesions, for example infarction, entrapment, and shingles [32, 33]. Complex regional pain syndrome (CRPS) is definitionally distinguished from post-traumatic neuralgia by additional visible signs, specifically “edema, changes in skin blood flow, or abnormal sudomotor activity in the region of the pain”  (Fig. 2). Many symptoms reflect microvascular dysfunction in a limb, and there is emerging evidence of a role for autoimmunity.
The 2003 Budapest diagnostic criteria for complex regional pain syndrome (CRPS), endorsed by the International Association for the Study of Pain in 2012. Distinctions between CRPS-I and CRPS-II are retained and a third subtype, CRPS-NOS (not otherwise specified) is recommended. Reproduced with permission from 
CRPS describes an array of painful conditions characterized by continuing (spontaneous and/or evoked) regional pain seemingly disproportionate in time or degree to the usual course of any known trauma or other lesion. The pain is regional (not in a specific nerve territory or dermatome) and usually has a distal predominance of abnormal sensory, motor, sudomotor, vasomotor, and/or trophic findings. The syndrome shows variable progression over time.
To make the clinical diagnosis the following criteria must be met:
1. Continuing pain, which is disproportionate to any inciting event
2. Must report at least 1 symptom in 3 of the 4 following categories:
Sensory: reports of hyperesthesia and/or allodynia
Vasomotor: reports of temperature asymmetry and/or skin color changes and/or skin color asymmetry
Sudomotor/edema: reports of edema and/or sweating changes and/or sweating asymmetry
Motor/trophic: reports of decreased range of motion and/or motor dysfunction (weakness, tremor, dystonia) and/or trophic changes (hair, nail, skin)
3. Must display at least 1 sign at time of evaluation in 2 or more of the following categories:
Sensory: evidence of hyperalgesia (to pinprick) and/or allodynia (to light touch and/or temperature sensation and/or deep somatic pressure and/or joint movement)
Vasomotor: evidence of temperature asymmetry (>1 °C) and/or skin color changes and/or asymmetry
Sudomotor/edema: evidence of edema and/or sweating changes and/or sweating asymmetry
Motor/trophic: evidence of decreased range of motion and/or motor dysfunction (weakness, tremor, dystonia) and/or trophic changes (hair, nail, skin)
4. There is no other diagnosis that better explains the signs and symptoms
For research purposes: must report at least 1 symptom in all 4 symptom categories and at least 1 sign (observed at evaluation) in 2 or more sign categories
Histological study shows no inflammatory cellular infiltrates in CRPS-affected tissues . The inflammation is chiefly neurogenic and microvascular . The distal limb is vulnerable because it lacks collateral circulation and blood must overcome gravity to exit. Leaky microvessels cause several of the characteristic signs of small-fiber damage: edema, sometimes including blisters, and abnormal color and temperature (Fig. 2) . The intravascular hemoconcentration associated with microvascular leaking promotes diapedesis of inflammatory cells into the limb. CRPS microvasculopathy has been attributed to malfunction and degeneration of the small- ibers innervating affected microvessels distal to the causal injury (Fig. 3) . Denervation of the arteriovenous shunts within CRPS-affected limbs permits blood to flow directly from the arterioles to the venules, bypassing the capillary beds and leaving the skin engorged with blood, while the deep tissues starve for oxygen and nutrients . This hypoxia triggers further inflammation and pain [53, 54].
The literature variably documents elevated levels of proinflammatory cytokines and other inflammatory markers in blood and tissues from patients with CRPS ; however, these abate over time . Few studies included other chronic pain controls so the findings may not be CRPS-specific [57, 58]. Limited evidence suggests that CRPS symptoms are not improved by treatment with nonsteroidal anti-inflammatory medications . Corticosteroids are effective for acute CRPS but not for most chronic cases . Adalimumab, a recombinant human monoclonal antibody that blocks tumor necrosis factor (TNF-α) binding was tested in an uncontrolled study of 10 patients with CRPS-I, with 6 responders . Case reports and a study of 42 patients reported efficacy of thalidomide, a tumor necrosis factor-α inhibitor , but a phase II, blinded RCT of lenalidomide, a safer thalidomide derivative, in 184 patients with CRPS-I did not find efficacy . Perhaps it would have been more effective if given earlier in the course.
Recent evidence implicates autoimmunity in CRPS . Sera from patients with CRPS has been reported to harbor autoantibodies targeting autonomic neurons [64, 65], and IgG extracted from these sera reproduce some CRPS features in mice . These early passive transfer experiments suggest roles for B cells and IgM , and IgG autoantibodies . Additionally, there is strong pathological and physiological evidence that focal injury to nerves sustained during surgery (which breaches the blood–nerve barrier) sometimes triggers autoimmune neuropathy . Although CRPS was not referenced, surgeries are a common trigger for CRPS, and it is logical to assume that nonsurgical traumas could also trigger antinerve autoimmunity. Also, one group has reported modest efficacy of immunomodulatory therapies for established refractory CRPS. Among 6 patients with long-standing CRPS, some benefited from therapeutic plasma exchange . One open-label study in 11 patients with CRPS and 1 well-designed, RCT in 12 patients reported modest efficacy of IVIg, 1 g/kg [70, 71]. Given the desperate situation of patients with refractory CRPS, some of whom resort to limb amputation to try and reduce their pain, larger trials of IVIg and other immunomodulatory therapies seem indicated.
The term “polyneuropathy” refers to widespread damage throughout the peripheral nerves. Polyneuropathies usually manifest at the distal ends of the axons, which are most vulnerable to disruptions in supply of energy or nutrients. Small-fiber polyneuropathy (SFPN) typically presents with chronic widespread pain starting in the feet, and/or dysautonomic symptoms, most often cardiovascular or gastrointestinal. The cardiovascular symptoms include not only orthostatic hypotension and tachycardia, but also impaired cognitive function, headache, and exercise intolerance due to circulatory insufficiency [72, 73, 74]. Gastrointestinal symptoms can be upper or lower, and reflect dysmotility and inadequate splanchnic circulation [75, 76]. SFPN cannot be detected by electromyography or standard nerve conduction studies, so special tests are needed for objective confirmation of diagnosis. Given the nonspecificity of most SFPN symptoms, objective confirmation is routine and usually required to obtain authorization for expensive treatments. Neurological societies endorse the use of biopsies of skin biopsies from the distal leg (Fig. 2), autonomic function testing (AFT), which detects autonomic and sudomotor small-fiber activity, and surgical biopsy of sensory nerves, usually the sural [77, 78].
Skin biopsy is the most accessible test as it can be performed in any medical facility and mailed to an accredited laboratory. The distal leg is biopsied 10 cm above the lateral malleolus, using subcutaneous bicarbonate-buffered lidocaine. After transporting the single 3-mm punch to the laboratory in the correct fixative, biopsies are vertically sectioned and immunolabeled against PGP9.5, a pan-neuronal marker that renders axons visible by light microscopy. As the epidermis is innervated almost exclusively by small fibers, measured innervation densities below the fifth centile of a normal distribution model are universally accepted as pathological confirmation of SFPN [77, 78]. A caveat is that each laboratory’s assessment of the normality of a patient’s measured density depends on the accuracy of their normative data and their statistical model. We reported that separate norms and modeling are needed to improve diagnostic accuracy in patients younger than their mid-20s . Distal leg skin biopsies have high positive predictive value but lower negative predictive value. A second biopsy from the thigh is sometimes added to improve sensitivity and inform about length dependence.
Like other polyneuropathies, SFPN is caused by underlying medical problems that must be identified for optimal treatment. Diabetes and toxic causes are best known , but several systemic autoimmune conditions are also associated . In addition to ganglionitis, Sjögren's also causes painful distal SFPN . In a series of 20 pathologically confirmed SS cases, one-third had pure SFN, one-third had mixed large and small fiber involvement, and one-third were not fully characterizable . My group found SS-A/SS-B antibodies in 9.2 % of the 98 tested patients with objectively verified SFPN . Various neuronal antigens have been implicated in Sjögren syndrome (SS) axonopathy, but verification has been elusive (reviewed in ). A French multicenter study documented efficacy of IVIg 2 g/kg for SS neuropathy, including sensorimotor axonopathy .
Celiac disease (sprue) is another autoimmune disease and distal SFPN, as well as ataxia and large-fiber polyneuropathy [12, 13]. Symptoms develop when susceptible individuals carrying high-risk human leukocyte antigen alleles ingest wheat, rye, or barley. The classical symptoms are chronic malabsorptive diarrhea, flatulence, iron deficiency anemia, and weight loss, but neurological manifestations are common, even in the roughly half with “silent celiac” who lack gastrointestinal symptoms. In a series of 409 British patients with celiac disease, 13 % had clinical and neurophysiological signs of sensory neuropathy . A pathological survey of > 28,000 Swedish patients with endoscopic verification of celiac identified a 2.8-times increased risk of chronic inflammatory demyelinating neuropathy, a 4.2-times increased risk of autonomic neuropathy, and a 7.6-times higher risk of mononeuritis multiplex, but no association with acute inflammatory demyelinating polyneuropathy . A study of 20 patients with idiopathic SFPN found that 8 % had endoscopically confirmed celiac disease . My group found anti-TTG-IgA antibody markers for celiac disease in 3.5 % of 109 patients with “idiopathic” SFPN .
Symptoms of celiac disease-associated neuropathy include asymmetrical numbness or paresthesias of the limbs, hands, feet, or face, and mild-to-moderate sensory ataxia, often with a nonlength-dependent distribution . Neurological manifestations appear mediated by both cellular and humoral responses, and autopsy study of celiac-affected dorsal root ganglia showed lymphocytic infiltrates with perivascular cuffing . Skin biopsies show reduced epidermal innervation (Fig. 2), often in a nonlength-dependent pattern . IgG antibodies against gliadin, IgG-deamidated gliadin peptide antibodies, IgA antibodies against endomysium, and IgA antibodies against different transglutaminases are also implicated (reviewed in ). Not all celiac neuropathy is immunological—malabsorptive vitamin deficiencies can contribute , although typically these present as cerebellar/ataxia rather than SFN. The cornerstone treatment, a lifelong gluten-free diet, is difficult. Immunotherapies are sometimes added for very ill patients , but there are no RCTs. One case series of 3 patients with biopsy-proven celiac disease reported that both their cerebellar ataxia and painful SFN improved after adding IVIg to their gluten-free diets .
This report brought referrals of other young patients, mostly with chronic courses, and we published a series of 41 patients with unexplained widespread chronic pain before the age of 21 years . Objective testing revealed definite SFPN in 59 % and probable SFPN in 17 % of cases. Among them, 98 % also had cardiovascular, gastrointestinal, or urological symptoms, documenting the unity of dysautonomic and somatic symptoms in SFPN. Exhaustive testing for causes of this “early-onset SFPN” identified only histories of autoimmune illnesses in 33 % of patients and serologic markers of disordered immunity in 89 %. Treatment with immunomodulatory therapy benefited 80 % of 15 treated patients . These reports tentatively characterize acute and chronic dysimmune forms of SFPN. The limited evidence available so far, such as normal cerebrospinal fluid, lack of cellularity within nerve biopsies, and presence of antinuclear antibodies and low serum complement C3 and C4, suggests innate and autoantibody-mediated immunity, most likely involving the classical or lectin rather than the alternative complement pathway .
These reports also provide a rationale for treating carefully selected patients with immunomodulatory therapies. Candidates should have symptoms consistent with SFPN, objective confirmation of diagnosis, exclusion of other causes, failure to improve with symptom management, and, ideally, serologic markers of immunity/inflammation. Given the lack of other options, and the disability of chronic widespread pain, particularly for children and young adults, it is reasonable to at least consider immunomodulatory treatments proven effective for other autoimmune neuropathies. In our series, among the 15 early-onset patients treated with 1–2-month trials of corticosteroids 67 % improved and 1 patient developed cataracts. Among the 11 early-onset patients treated at least 3 times with IVIg, 2 g/kg/month, 38 % did not respond and discontinued treatment, and 62 % had significant improvement and continued treatment . Adverse events were as expected and largely manageable. Repeat skin biopsies and AFT provided objective confirmation of axonal regeneration.
We recently reviewed the outcomes of 46 consecutive patients of all ages with objectively confirmed SFPN, dysimmune attribution, and treatment with ≥ 1 cycle of IVIg at 2 g/kg for 4 weeks; the primary outcome was change in pain . Among the 31 with baseline pain scores ≥ 3, pretreatment pain was significantly reduced, and among the 28 patients with pre- and post-treatment AFT, the high prevalence of abnormal AFT at baseline was halved. Patients’ global impression-of-change scores showed that > 70 % rated themselves as improved . Thus, the preliminary evidence supports the use of short-term corticosteroids and long-term IVIg for carefully selected patients with SFPN. These retrospective data also provide a rationale for conducting prospective studies and for defining the mechanisms of autoimmune SFPN.
A new discovery is that roughly half of patients with fibromyalgia have objective evidence of SFPN. The label “fibromyalgia” describes a symptom complex that previously lacked any link to pathobiology, leaving practitioners attributing it to psychopathology or unspecified “central sensitization”. As fibromyalgia affects several percent of the population, the public health and economic implications are considerable. Initial pathology skin biopsy studies identifying loss of cutaneous innervation were followed by electrophysiologic evidence that C-fibers fire spontaneously and excessively in patients with fibromyalgia as in positive-control patients with SFPN but not normal controls [74, 92, 93, 94]. Subsequent studies have so far all been supportive (e.g., ), so biological overlap between fibromyalgia and SFPN appears confirmed. “Fibromyalgia” is a label applied to a family of multisymptom disorders with different underlying causes, and it may be superseded, as the old term “pulmonary congestion” was replaced by specific pathological diagnoses, for example heart failure, tuberculosis, and lung cancer.
This new discovery is not yet widely known but requests to evaluate patients with fibromyalgia for SFPN are already rising. They seem justified as SFPN has identifiable underlying causes that can sometimes be definitively treated and occasionally cured. However, neither skin biopsy nor microneurography are widely available, so simpler diagnostic tools are needed. The implications for immunotherapy are net yet certain. A proportion of patients with fibromyalgia have evidence for inflammation/dysimmune mechanisms by virtue of also having systemic autoimmune diseases, dysimmune profiles in blood or skin, and/or improvement in fibromyalgia symptoms after immunotherapy . Comparing patients with fibromyalgia with those without systemic autoimmune rheumatologic disorders revealed that 44 % of those with systemic autoimmunity versus 27 % of those without it had skin biopsies consistent with SFPN . Thus, it may be reasonable to more seriously consider immunotherapy for the subset of patients with fibromyalgia with confirmed SFPN, evidence of immune mediation, exclusion of other causes, and inadequate response to symptom-based treatments.
The development of objective methods of detecting small-fiber loss, most notably skin biopsies immunolabeled to reveal small-fiber innervation (Fig. 2), is beginning to improve awareness and detection of small-fiber illnesses. They are more common and diverse than appreciated. There is now preliminary evidence of dysimmune causes of some neuropathies that present primarily with neuropathic pain or dysautonomic symptoms, particularly in patients with Sjögren, celiac disease, or paraneoplastic syndromes. Limited data now suggest that dysimmune SFNs respond to immunotherapies like other autoimmune neurological conditions. Given the distress, disability, high cost, and prevalence of chronic pain and widespread multisymptom illnesses, the need to establish and validate outcome measures, verify the proportion of illnesses caused by dysimmunity, dissect molecular mechanisms, and conduct prospective trials of immunotherapies is becoming evident.
This work was supported, in part, by the National Institutes of Health (K24NS059892, R01NS093653). I acknowledge the mentorship of the late John W. Griffin MD with gratitude.
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- 12.Hadjivassiliou M, Rao DG, Wharton SB, Sanders DS, Grunewald RA, Davies-Jones AGB. Sensory ganglionopathy due to gluten sensitivity. Neurology 2010 14;75:1003–1008.Google Scholar
- 13.Brannagan TH, III, Hays AP, Chin SS, et al. Small-fiber neuropathy/neuronopathy associated with celiac disease: skin biopsy findings. Arch Neurol 2005 1;62:1574–1578.Google Scholar
- 14.Sene D, Cacoub P, Authier FJ, et al. Sjögren syndrome-associated small fiber neuropathy: Characterization from a prospective series of 40 cases. Medicine 2013 Aug 26 [Epub ahead of print].Google Scholar
- 17.Chai J, Herrmann DN, Stanton M, Barbano RL, Logigian EL. Painful small-fiber neuropathy in Sjögren's syndrome. Neurology 2005 27;65:925–927.Google Scholar
- 34.Merskey H, Bogduk N. Classification of chronic pain: descriptions of chronic pain syndromes and definitions of pain terms. 2nd ed. IASP Press, Seattle, 1994.Google Scholar
- 35.Mitchell SW. Injuries of nerves and their consequences. Dover Publications, American Academy of Neurology Reprint Series (1965 edition), New York, 1864.Google Scholar
- 36.Mitchell SW, Morehouse GR, Keen WM. Gunshot wounds and other injuries of nerves. J.B. Lippincott, Philadelphia, PA, 1864.Google Scholar
- 37.Sudeck PHM. Über die akute (reflektorische) Knochenatrophie nach Entzündungen und Verletzungen an den Extremitäten und ihre klinischen Erscheinungen. Fortschritte auf dem Gebiete der Röntgenstrahlen 1901;5:277.Google Scholar
- 55.Parkitny L, McAuley JH, Di Pietro F, et al. Inflammation in complex regional pain syndrome: A systematic review and meta-analysis. Neurology 2013 1;80:106–117.Google Scholar
- 58.Üçeyler N, Rogausch JP, Toyka KV, Sommer C. Differential expression of cytokines in painful and painless neuropathies. Neurology 2007 3;69:42–49.Google Scholar
- 61.Schwartzman RJ, Chevlen E, Bengtson K. Thalidomide has activity in treating complex regional pain syndrome. Arch Intern Med 2003 23;163:1487–1488.Google Scholar
- 71.Goebel A, Baranowski A, Maurer K, Ghiai A, McCabe C, Ambler G. Intravenous immunoglobulin treatment of the complex regional pain syndrome: a randomized trial. Ann Intern Med 2010 2;152:152–158.Google Scholar
- 77.England JD, Gronseth GS, Franklin G, et al. Practice Parameter: Evaluation of distal symmetric polyneuropathy: role of autonomic testing, nerve biopsy, and skin biopsy (an evidence-based review). Report of the American Academy of Neurology, American Association of Neuromuscular and Electrodiagnostic Medicine, and American Academy of Physical Medicine and Rehabilitation. Neurology 2009 13;72:177–184.Google Scholar
- 78.Lauria G, Hsieh ST, Johansson O, et al. European Federation of Neurological Societies/Peripheral Nerve Society Guideline on the use of skin biopsy in the diagnosis of small fiber neuropathy. Report of a joint task force of the European Federation of Neurological Societies and the Peripheral Nerve Society. Eur J Neurol 2010;17:903–909.CrossRefPubMedGoogle Scholar
- 79.Klein MM, Downs H, O'Neil K., Oaklander AL. Skin biopsy of normal children demonstrates inverse correlation between age and epidermal nerve fiber density, meaning age-specific norms are needed. Ann Neurology 2014;76:S69Google Scholar
- 82.Lang M, Treister R, Oaklander AL. Cost/benefit of blood tests in idiopathic small-fiber polyneuropathy (SFPN). Ann Neurol 2015;78:S19.Google Scholar
- 85.Chin RL, Sander HW, Brannagan TH, et al. Celiac neuropathy. Neurology 2003 27;60:1581–1585.Google Scholar
- 91.Oaklander AL, Lang M, Treister R, Liu X. First evaluation of immunoglobulin treatment of small-fiber axonal polyneuropathy (SFPN). Ann Neurol 2015;78:S109.Google Scholar