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AMBIO

, Volume 41, Issue 5, pp 456–466 | Cite as

Growth and Histopathological Effects of Chronic Exposition of Marine Pejerrey Odontesthes argentinensis Larvae to Petroleum Water-Soluble Fraction (WSF)

  • Emeline Pereira Gusmão
  • Ricardo Vieira Rodrigues
  • Cauê Bonucci Moreira
  • Luis Alberto Romano
  • Luís André Sampaio
  • Kleber Campos Miranda-Filho
Report

Abstract

The water-soluble fraction (WSF) of petroleum contains a mixture of polycyclic aromatic hydrocarbons, volatile hydrocarbons, phenols, and heterocyclic compounds, considered deleterious to aquatic biota. Marine “pejerrey” Odontesthes argentinensis (Teleostei: Atherinopsidae) has a great commercial importance in local fisheries and a high potential for aquaculture. The aim of this study was to evaluate the histopathological effects in “pejerrey” larvae exposed to different concentrations of petroleum WSF. The chronic toxicity test was conducted with newly hatched larvae exposed for 21 days to sublethal concentrations of WSF (2.5, 5, 10, and 20 % of WSF), plus one control. Survival and growth were significantly lower in the highest concentration. Several histopathological changes were found in the gills (e.g., hyperplasia, aneurisms, edema, and necrosis), kidney (e.g., nuclear alterations, decrease in the hematopoietic cells), and liver (e.g., hypertrophy, karyorrhexis, and karyopyknosis). An index of branchial lesion was proposed to standardize gill lesions to different pollutants.

Keywords

Toxicology Petroleum WSF “Pejerrey” Histopathology Lesion index 

Notes

Acknowledgments

The authors would like to gratefully acknowledge the Brazilian National Agency of Petroleum (ANP) for supporting the students E.P. Gusmão, R.V. Rodrigues, and C.B. Moreira. K.C. Miranda-Filho thanks CNPq (481613/2007-2) and CAPES for their support. L.A. Sampaio is a research fellow of Brazilian CNPq (308013/2009-3).

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Copyright information

© Royal Swedish Academy of Sciences 2012

Authors and Affiliations

  • Emeline Pereira Gusmão
    • 1
  • Ricardo Vieira Rodrigues
    • 1
  • Cauê Bonucci Moreira
    • 1
  • Luis Alberto Romano
    • 2
  • Luís André Sampaio
    • 2
  • Kleber Campos Miranda-Filho
    • 3
  1. 1.Programa de Pós-Graduação em Aquicultura, Laboratório de Piscicultura Estuarina e MarinhaUniversidade Federal do Rio GrandeRio GrandeBrazil
  2. 2.Laboratório de Piscicultura Estuarina e Marinha, Instituto de OceanografiaUniversidade Federal do Rio GrandeRio GrandeBrazil
  3. 3.Escola de Veterinária, Departamento de Zootecnia, Laboratório de AquaculturaUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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