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Highly expressed lncRNA CRNDE promotes cell proliferation through Wnt/β-catenin signaling in renal cell carcinoma

Tumor Biology volume 37, pages 15997–16004 (2016)Cite this article

Abstract

Recently, numerous studies revealed that long non-coding RNAs (lncRNAs) play complex roles in the field of tumor biology, while the functions of lncRNA in renal cell carcinoma (RCC) remain largely unknown. In the current study, we retrieved Oncomine database and found a lncRNA colorectal neoplasia differentially expressed (CRNDE) which is highly expressed in different cohorts of RCC patients; this clue reminds us that CRNDE might exert its functions in RCC tumorigenesis. We then detected the level of CRNDE in fresh RCC tissues and found that CRNDE is significantly up-regulated compared with adjacent tissues. Furthermore, both loss and gain function assays revealed that CRNDE promotes RCC cell proliferation and growth both in vitro and in vivo.

In addition, we found that CRNDE regulates the cell cycle transition from G0/G1 stage to S stage and modulates the expression of CCND1 and CCNE1. Moreover, we further illustrated that CRNDE activates Wnt/β-catenin signaling in RCC cell lines. Taken together, in the current study, we found that lncRNA CRNDE is highly expressed in RCC malignant tissues and the heightened CRNDE robustly promotes RCC cell proliferation through activating Wnt/β-catenin signaling; our findings enlarge our knowledge in the molecular pathology of RCC tumorigenesis.

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Acknowledgments

We thank Dr. He’s for the constructive suggestions in the experiment design and the preparation of the manuscript.

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Affiliations

  1. Department of Urology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai, 200025, China

    Kun Shao, Tianming Shi, Yang Yang, Xianghui Wang, Da Xu & Peijun Zhou

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  1. Kun Shao
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  2. Tianming Shi
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  3. Yang Yang
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  4. Xianghui Wang
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  5. Da Xu
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  6. Peijun Zhou
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Correspondence to Peijun Zhou.

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Additional information

Kun Shao and Tianming Shi contributed equally to this manuscript.

Electronic supplementary material

Figure 1S

(A) The expression of CRNDE is significantly down-regulated in 786-O and A498 cell lines. (B) Overexpression of CRNDE dramatically elevates the expression of CRNDE in HEK-293T cell lines (JPEG 174 kb)

Figure 2S

3X Flag tagged CRNDE validated the non-coding potential of CRNDE. Western blots shown that CRNDE and positive control UCA1 could not be determined by Flag antibody, while negatively control LKB1 could be examined (JPEG 68 kb)

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Shao, K., Shi, T., Yang, Y. et al. Highly expressed lncRNA CRNDE promotes cell proliferation through Wnt/β-catenin signaling in renal cell carcinoma. Tumor Biol. 37, 15997–16004 (2016). https://doi.org/10.1007/s13277-016-5440-0

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  • DOI: https://doi.org/10.1007/s13277-016-5440-0

Keywords

  • CRNDE
  • Cell proliferation
  • Wnt/β-catenin signaling
  • Renal cell carcinoma