Tumor Biology

, Volume 37, Issue 12, pp 15813–15823 | Cite as

Association of single-nucleotide polymorphisms of high-mobility group box 1 with susceptibility and clinicopathological characteristics of uterine cervical neoplasia in Taiwanese women

  • Hsin-Hung Wu
  • Yu-Fan Liu
  • Shun-Fa Yang
  • Wea-Lung Lin
  • Shiuan-Chih Chen
  • Chih-Ping Han
  • Hsiang-Ling Wang
  • Long-Yau Lin
  • Po-Hui Wang
Original Article


To date, no study associated the genetic polymorphisms of high-mobility group box 1 protein (HMGB1) with the development of uterine cervical cancer. We therefore conducted this study to investigate the associations of HMGB1 single-nucleotide polymorphisms (SNPs) with cervical carcinogenesis and clinicopathological characteristics of cancer patients. Five hundred two women, including 112 with invasive cancer, 85 with precancerous lesions of the uterine cervix, and 305 normal controls, were consecutively enrolled into this study. Analysis of HMGB1 SNPs was done by real-time polymerase chain reaction and genotyping. Our results found that the risk of susceptibility to cervical invasive cancer was 1.85 (95 % CI 1.12–3.04; p = 0.016) in women with TC and 1.99 (95 % CI 1.24–3.23; p = 0.005) in women with TC/CC after adjusting for age, using TT as a comparison reference in HMGB1 SNP rs1412125. In rs2249825, the increased risk was also seen for the development of cervical invasive cancer in women with CG [adjusted odds ratio (AOR) 2.04, 95 % CI 1.22–3.40; p = 0.006] or CG/GG (AOR 2.02, 95 % CI 1.22–3.32; p = 0.006) using CC as a comparison reference. An additional integrated in silico analysis confirmed that rs2249825 creates a binding site for v-Myb, which may affect HMGB1 expression. In conclusion, Taiwanese women with TC or TC/CC in HMGB1 SNP rs1412125 as well as CG or CG/GG in rs2249825 were susceptible to the development of cervical invasive cancer.


Single-nucleotide polymorphism HMGB1 Cervical cancer 


Compliance with ethical standards

The Chung Shan Medical University Hospital Institutional Review Board approved this study, and informed written consents were obtained from all subjects (CSMUH IRB: CS12219, CS14014)

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Hsin-Hung Wu
    • 1
    • 2
    • 3
  • Yu-Fan Liu
    • 4
    • 5
  • Shun-Fa Yang
    • 1
    • 5
  • Wea-Lung Lin
    • 6
    • 7
  • Shiuan-Chih Chen
    • 1
    • 6
    • 8
  • Chih-Ping Han
    • 6
    • 7
    • 9
  • Hsiang-Ling Wang
    • 1
    • 10
  • Long-Yau Lin
    • 1
    • 6
    • 9
  • Po-Hui Wang
    • 1
    • 6
    • 9
  1. 1.Institute of MedicineChung Shan Medical UniversityTaichungTaiwan
  2. 2.Infertility CenterXiamen EMBO Hospital XiamenFujianChina
  3. 3.Reproductive Medicine CenterKinmen HospitalKinmenTaiwan
  4. 4.Department of Biomedical SciencesChung Shan Medical UniversityTaichungTaiwan
  5. 5.Department of Medical ResearchChung Shan Medical University HospitalTaichungTaiwan
  6. 6.School of MedicineChung Shan Medical UniversityTaichungTaiwan
  7. 7.Department of PathologyChung Shan Medical University HospitalTaichungTaiwan
  8. 8.Department of Family and Community MedicineChung Shan Medical University HospitalTaichungTaiwan
  9. 9.Department of Obstetrics and GynecologyChung Shan Medical University HospitalTaichungTaiwan
  10. 10.Department of Beauty ScienceNational Taichung University of Science and TechnologyTaichungTaiwan

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