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Tumor Biology

, Volume 37, Issue 11, pp 14961–14967 | Cite as

The predictive potential and oncogenic effects of HOXC8 expression on osteosarcoma

  • Li Cheng
  • Xiaojie Wei
  • Keqi Zhao
  • Fenzan Wu
  • Wenjie Lu
  • Songlin Tong
  • Guanzhen Yu
Original Article
  • 125 Downloads

Abstract

Homeobox C8 (HOXC8) has been implicated in cell growth, migration, and metastasis of various cancers, yet its role in osteosarcoma remains to be explored. In the present study, resected osteosarcoma specimens from 50 patients were enrolled to evaluate the expression of HOXC8 protein by immunohistochemistry (IHC). In vitro and in vivo assays were used to determine the effect of HOXC8 on cell growth, migration, and tumor growth. HOXC8 expression was observed in 31 (62.0 %) of the 50 primary tumors and significantly associated with poorly or un-differentiated specimens (P = 0.031) and larger tumor size (P = 0.049). Survival analysis demonstrated that HOXC8 is a candidate predictive factor in predicting patients’ outcome and chemotherapeutic effect. HOXC8 knockdown led to inhibition of tumor cell proliferation and migration in vitro by inhibiting MMP-9 expression and tumor growth in vivo. Our results strongly suggest that HOXC8 is involved in the tumorigenesis of osteosarcoma and might serve as a novel predictor for patients’ outcome.

Keywords

Osteosarcoma HOXC8 Prognosis Immunohistochemistry Chemotherapeutic effect 

Notes

Compliance with ethical standards

The Institutional Review Board of Changzheng Hospital approved the use of the tissues and clinical information. An informed consent was obtained from each patient or their guardians. All animal experiments were approved by the Animal Ethics Committee of the Second Military Medical University.

Conflict of interest

We declare no conflicts of interest with any other person or units.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Li Cheng
    • 1
  • Xiaojie Wei
    • 1
  • Keqi Zhao
    • 1
  • Fenzan Wu
    • 1
  • Wenjie Lu
    • 1
  • Songlin Tong
    • 1
  • Guanzhen Yu
    • 2
  1. 1.Department of Orthopaedics, Cixi People’s HospitalWenzhou Medical UniversityNingboChina
  2. 2.Department of OncologyChangzheng HospitalShanghaiChina

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