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Dendritic cells loading autologous tumor lysate promote tumor angiogenesis

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Tumor Biology

Abstract

Dendritic cells (DC) have been exploited for vaccination against cancer for years. DC loading autologous tumor lysate (ATL-DC) have been assessed in ongoing clinical trials, but frequently do not meet expectation. In this study, we found that mice immunized with ATL-DC induced less protective anti-tumor effect than immunized with DC alone. The percentage of CD8+ T cells and the lysis efficiency of CTLs to auto tumor cells in ATL-DC vaccination group was less than that of DC group. Moreover, vaccination of mice with ATL-DC also promoted tumor angiogenesis by analyzing the CD31 positive microvessel density and hemoglobin content of tumor specimens. Human umbilical vein endothelial cells (HUVEC) have been proved effective in the anti-angiogenesis immunity against cancer. However, in the following research we found that the anti-tumor effect was attenuated while immunized mice with HUVEC combined with ATL-DC (HUVEC + ATL-DC). Furthermore, immunized mice with HUVEC + ATL-DC profoundly increased the tumor angiogenesis by analyzing the microvessel density and hemoglobin content of tumor specimens. These data suggest that vaccination using ATL-DC antagonized HUVEC induced anti-angiogenesis effect. Our research for the first time indicated that ATL-DC have the potential to promote the process of tumor angiogenesis in vivo. As vaccines based on DC loading autologous tumor lysate have been used in clinical, this find warned that the safety of this kind of vaccine should be taken into consideration seriously.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81572972), Science and technology innovation talents support plan of university in Henan province (no. 15HASTIT038), and Science Foundation of Zhengzhou University for the Excellent young teacher (no. 1421328057).

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Authors and Affiliations

  1. Department of Pathophysiology, Basic Medical College, Zhengzhou University, No. 100 Science Road, Zhengzhou, 450001, Henan, China

    Yi Yang, Jing Lu, Hangfan Liu, Guoguo Jin, Xiang Li, Dongyu Wang, Jimin Zhao, Youtian Huang, Kangdong Liu & Ziming Dong

  2. Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan, 450001, China

    Yi Yang, Jing Lu, Hangfan Liu, Guoguo Jin, Xiang Li, Dongyu Wang, Jimin Zhao, Youtian Huang, Kangdong Liu & Ziming Dong

  3. Department of Physiology, Basic Medical College, Zhengzhou University, Zhengzhou, Henan, 450001, China

    Yi Yang & Ying Xing

  4. Department of Pathology, Henan Cancer Hospital, Zhengzhou University, Zhengzhou, Henan, 450008, China

    Ruihua Bai

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  1. Yi Yang
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Correspondence to Ying Xing or Ziming Dong.

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Yi Yang, Jing Lu, and Hangfan Liu contributed equally to the manuscript.

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Yang, Y., Lu, J., Liu, H. et al. Dendritic cells loading autologous tumor lysate promote tumor angiogenesis. Tumor Biol. 37, 15687–15695 (2016). https://doi.org/10.1007/s13277-016-5312-7

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  • DOI: https://doi.org/10.1007/s13277-016-5312-7

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