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MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene

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Tumor Biology

Abstract

MicroRNAs can affect behaviors of tumor cells by modulating the expression of the target genes that involve tumor growth, invasiveness, and death. The goal of this research is to examine the effects of miR-15a on the proliferation and invasiveness of malignant melanoma cells in vitro, as well as the therapeutic effect of miR-15a in a mouse melanoma model. miR-15a displayed inhibitory effects on proliferation and invasiveness of several malignant melanoma cell lines. miR-15a also caused cell cycle arrest at G1/G0 phase. miRNA 15a downregulated the expressions of CDCA4 and AKT-3 in melanoma cell lines. In vivo, experiment showed that miRNA 15a significantly retarded the growth of melanoma tumors in the mouse model. The luciferase reporter assay demonstrated that miR15a can suppress gene expression through the binding site in the 3 ′UTR of CACD4, which is a bona fide target of miRNA 15a. In conclusion, miRNA 15a suppressed the growth and invasiveness of melanoma cells, suggesting that miRNA 15a may represent a viable microRNA-based therapy against melanoma.

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Acknowledgments

This project was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20 GM103418 (PI: Dr. Yixin Yang) and the Emporia State University Faculty Research and Creativity Grant (PI: Dr. Yixin Yang). This project was also supported by the grants from the National Natural Science Foundation of China (Beijing, People’s Republic of China) with the grant number of 81471373 (PI: Dr. Zhaoyang Xiao) and the Liaoning Province Natural Science Foundation with the grant number of 2014023007 (PI: Dr. Zhaoyang Xiao).

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Authors and Affiliations

  1. Department of Biological Sciences, Emporia State University, 1 Kellogg Circle, Emporia, KS, USA

    Christopher Alderman, Ayoub Sehlaoui & Yixin Yang

  2. Department of Emergency Medicine, The 2nd Affiliated Hospital of Dalian Medical University, No. 467 Zhongshan Road, Dalian, China

    Zhaoyang Xiao

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  1. Christopher Alderman
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Correspondence to Zhaoyang Xiao or Yixin Yang.

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The authors (Christopher Alderman, Ayoub Sehlaoui, Zhaoyang Xiao, Yixin Yang) of the manuscript entitled “MicroRNA-15a Inhibits the Growth and Invasiveness of Malignant Melanoma and Directly Targets on CDCA4 Gene” have NO affiliations with or involvement in any organization or entity with any financial interest, or non-financial interest in the subject matter or materials discussed in this manuscript.

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Christopher Alderman and Ayoub Sehlaoui are equal contributors to this project.

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Alderman, C., Sehlaoui, A., Xiao, Z. et al. MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene. Tumor Biol. 37, 13941–13950 (2016). https://doi.org/10.1007/s13277-016-5271-z

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  • DOI: https://doi.org/10.1007/s13277-016-5271-z

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