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Tumor Biology

, Volume 37, Issue 9, pp 12855–12866 | Cite as

Overexpression of MYCN promotes proliferation of non-small cell lung cancer

  • Kun Liu
  • Shuo Wang
  • Yifei Liu
  • Jun Gu
  • Shudong Gu
  • Zhen Xu
  • Rui Zhang
  • Zhiwen Wang
  • Huaci Ma
  • Yingying Chen
  • Lili Ji
Original Article

Abstract

V-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (MYCN) is an oncogene that is known amplified and overexpressed in different human malignancies including small cell lung cancer. However, the role of MYCN in non-small cell lung cancer (NSCLC) development remains elusive. In the present study, Western blot and immunohistochemistry assays demonstrated that MYCN was overexpressed in NSCLC tumor tissues and cell lines. In addition, immunohistochemistry analysis revealed that upregulation of MYCN expression was positively correlated with a more invasive tumor phenotype and poor prognosis. In vitro studies using serum starvation-refeeding experiment and MYCN-siRNA transfection assay demonstrated that MYCN expression promoted proliferation of NSCLC cells, while MYCN knockdown led to decreased cell growth resulted from growth arrest of cell cycle at G0/G1 phase. Furthermore, upregulation and knockdown of sex-determining region Y-box 2 (SRY) (SOX2), which was a well-known oncogene, confirmed that MYCN might be a downstream gene of the transcription factor SOX2. Collectively, our finding suggested that MYCN might contribute to the progression of NSCLC by enhancing cell proliferation, and that targeting MYCN might provide beneficial effects for the clinical therapy of NSCLC.

Keywords

MYCN SOX2 NSCLC Cell cycle Proliferation 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81501975), University Science Research Project of Jiangsu Province (15KJB310013), Six Talent Peaks Project in Jiangsu Province (nos. 2014-YY-006 and WSN-059), Nantong Science and Technology Project (MS12015103 and HS2012025), and Nantong University Innovation Project (YKC15087).

Compliance with ethical standards

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Kun Liu
    • 1
  • Shuo Wang
    • 1
    • 2
    • 3
  • Yifei Liu
    • 4
  • Jun Gu
    • 5
  • Shudong Gu
    • 6
  • Zhen Xu
    • 6
  • Rui Zhang
    • 5
  • Zhiwen Wang
    • 2
  • Huaci Ma
    • 7
  • Yingying Chen
    • 3
    • 6
  • Lili Ji
    • 2
    • 3
  1. 1.Department of Cardiothoracic SurgeryAffiliated Hospital of Nantong UniversityNantongChina
  2. 2.Department of PathologyMedical School of Nantong UniversityNantongChina
  3. 3.Jiangsu Province Key Laboratory for Inflammation and Molecular Target, Medical School of Nantong UniversityNantongChina
  4. 4.Department of PathologyAffiliated Hospital of Nantong UniversityNantongChina
  5. 5.Department of RespiratoryAffiliated Hospital of Nantong UniversityNantongChina
  6. 6.Department of OncologyAffiliated Hospital of Nantong UniversityNantongChina
  7. 7.Department of OtorhinolaryngologyAffiliated Hospital of Nantong UniversityNantongChina

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