Poly r(C) binding protein is post-transcriptionally repressed by MiR-490-3p to potentiate squamous cell carcinoma
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Squamous cell skin carcinoma remains a leading cause of cancer-related mortality with a huge cost of treatment, necessitating discovery and validation of potent therapeutic targets. Poly r(C) binding protein 1 (PCBP1) has been previously shown to function as a tumor suppressor. Previous work has shown that PCBP1 expression is inversely correlated to maintenance of cancer stem cells in squamous cell skin carcinoma and prostate cancer, respectively. However, the precise mechanism that regulates PCBP1 expression has not been elucidated. Here, we show that loss of PCBP1 protein expression observed in CD34+ COLO-16 cells is orchestrated by translational silencing. Translational silencing is caused by targeting of PCBP1 mRNA by miR-490-3p. Exogenous manipulation of miR-490-3p levels can accordingly modulate PCBP1 protein expression, thus suggesting that miR-490-3p as a potential biomarker in squamous cell skin cancer with therapeutic benefits.
KeywordsPoly r(C) binding protein MiR-490-3p Potentiate squamous cell carcinoma
Compliance with ethical standards
All procedures performed in studies involving human participants were in accordance with the ethical standards of the Chinese PLA General Hospital, China and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained for by each patient included.
Conflict of interest
- 10.Brown AS, Mohanty BK, Howe PH. Identification and characterization of an hnRNP E1 translational silencing motif. Nucleic Acids Res. 2016 Apr 11. pii: gkw241Google Scholar
- 11.Zhang ZZ, Shen ZY, Shen YY, Xu J, Zhao EH, Wang M, Wang CJ, Cao H. HOTAIR long noncoding RNA promotes gastric cancer metastasis through suppression of Poly r(C) Binding Protein (PCBP) 1. Mol Cancer Ther. 2015 Jan 22. pii: molcanther.0695.2014. [Epub ahead of print].Google Scholar
- 19.Xue X, Wang X, Liu Y, Teng G, Wang Y, Zang X, et al. SchA-p85-FAK complex dictates isoform-specific activation of Akt2 and subsequent PCBP1-mediated post-transcriptional regulation of TGFβ-mediated epithelial to mesenchymal transition in human lung cancer cell line A549. Tumour Biol. 2014;35(8):7853–9.CrossRefPubMedGoogle Scholar
- 29.Alečković M, Kang Y. Regulation of cancer metastasis by cell-free miRNAs. Biochim Biophys Acta. 1855;2014:24–42.Google Scholar