MiR-384 regulated IRS1 expression and suppressed cell proliferation of human hepatocellular carcinoma
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Acquired evidence indicated that microRNAs (miRNAs) played essential roles in cancer development, including hepatocellular carcinoma (HCC). Functions and mechanisms of miRNAs involved in HCC remain largely unknown. Here, we found that miR-384 was significantly downregulated in HCC cells and tissues by RT-PCR. Gain and loss of function studies revealed that miR-384 significantly suppressed HCC cell proliferation. Insulin receptor substrate 1(IRS1) was identified as a direct and functional target of miR-384. Moreover, miR-384 decreased IRS1 expression, subsequently downregulating cyclin D1 and upregulating p21 and p-Rb expression. In addition, promotion of cell proliferation caused by miR-384-in was counteracted by silencing IRS1 expression with siRNAs. Taken together, our data provided convincing evidence that miR-384 exerted suppressive effect on HCC cell proliferation through the direct inhibition of IRS1 expression, suggesting miR-384 may serve as a potential therapeutic target for HCC.
KeywordsmiR-384 Human hepatocellular carcinoma IRS1 Cell proliferation
This work was supported by Guangzhou medicine and health care technology projects (20141A011011, 20151A011007 and 20161A011008), Guangzhou science and technology plan projects (201510010009) and Guangdong provincial science and technology plan projects (2014A020212033). All authors designed the study together, performed the experiment together, analyzed the data and wrote the paper; all authors approved the final manuscript.
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Conflicts of interest