Tumor Biology

, Volume 37, Issue 10, pp 13225–13235 | Cite as

Depletion of UBA protein 2-like protein inhibits growth and induces apoptosis of human colorectal carcinoma cells

  • Rui Chai
  • Xiaojun Yu
  • Shiliang Tu
  • Bo’an Zheng
Original Article


Ubiquitin-proteasome system regulates cell proliferation, apoptosis, angiogenesis, and motility, which are processes with particular importance for carcinogenesis. UBA protein 2-like protein (UBAP2L) was found to be associated with proteasome; however, its biological function is largely unknown. In this study, the mRNA levels of UBAP2L in human normal and colorectal carcinoma tissues were analyzed using the datasets from the publicly available Oncomine database ( and found UBAP2L was overexpressed in colorectal carcinoma tissues. Furthermore, we elucidated the role of UBAP2L in human colorectal cancer via an RNA interference lentivirus system in three colorectal carcinoma cell lines HCT116, SW1116, and RKO. Knockdown of UBAP2L led to suppressed cell proliferation and impaired colony formation. UBAP2L depletion in HCT116 and RKO cells also induced cell cycle arrest as well as apoptosis. Moreover, the phosphorylation of PRAS40, Bad, and the cleavage of PARP were remarkably increased after UBAP2L knockdown by Intracellular signaling array and also the activation of P38 was obviously decreased and the cleavage of Caspase 3 and Bax were increased after UBAP2L silencing by western blot assay, indicated that UBAP2L might be involved in the cell growth by the regulation of apoptosis-related proteins. Our findings indicated that UBAP2L may be essential for colorectal carcinoma growth and survival. Lentivirus-mediated small interfering RNA against UBAP2L might serve as a potential therapeutic approach for the treatment of colorectal cancer.


Apoptosis Colorectal cancer Proliferation siRNA UBAP2L 



The authors are thankful for the financial support from Natural Science Foundation of Zhejiang province (grant no. Y15H160152).

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Rui Chai
    • 1
  • Xiaojun Yu
    • 2
  • Shiliang Tu
    • 1
  • Bo’an Zheng
    • 1
  1. 1.Department of Colorectal SurgeryZhejiang Provincial People’s HospitalHangzhouChina
  2. 2.Department of Gastroenterological SurgeryZhejiang Provincial People’s HospitalHangzhouChina

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