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Tumor Biology

, Volume 37, Issue 10, pp 13533–13543 | Cite as

P2RX7-V3 is a novel oncogene that promotes tumorigenesis in uveal melanoma

  • Hui Pan
  • Hongyan Ni
  • LeiLei Zhang
  • Yue Xing
  • Jiayan Fan
  • Peng Li
  • Tianyuan Li
  • Renbing Jia
  • Shengfang Ge
  • He Zhang
  • Xianqun Fan
Original Article

Abstract

Uveal melanoma (UM) has a high mortality rate for primary intraocular tumors. Approximately half of UM patients present with untreatable and fatal metastases. Long non-coding RNAs (lncRNAs) have emerged as potent regulatory RNAs that play key roles in various cellular processes and tumorigenesis. However, to date, their roles in UM are not well-known. Here, we identified a transcriptional variant transcribed from the P2RX7 gene locus, named P2RX7-V3 (P2RX7 variant 3), which was expressed at a high level in UM cells. P2RX7-V3 silencing revealed that this variant acts as a necessary UM oncoRNA. Knockdown of P2RX7-V3 expression significantly suppressed tumor growth in vitro and in vivo. A genome-wide cDNA array revealed that a variety of genes were dysregulated following P2RX7-V3 silencing. These observations identified P2RX7-V3 that plays a crucial role in UM tumorigenesis and may serve as a useful biomarker in the diagnosis and prognosis treatment of UM in the future.

Keywords

Uveal melanoma P2RX7 Variant lncRNA Metastasis oncoRNA 

Notes

Acknowledgments

This work was supported by Scientific Research Program of The National Health and Family Planning Commission of China (201402014) for XQF, The National Natural Science Foundation of China (grant 31470757), The Program for Professor of Special Appointment (Eastern Scholar) at the Shanghai Institutions of Higher Learning (1410000159), The Shanghai PuJiang Program (13PJ1405700), SMC-ChenXing Yong Scholar Program (2014, Class B), and The Science and Technology Commission of Shanghai (grants 14JC1404100, 14JC1404200, 14430723100). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Compliance with ethical standards

Conflicts of interest

None.

Supplementary material

13277_2016_5141_MOESM1_ESM.doc (30 kb)
Table S1 (DOC 30 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Hui Pan
    • 1
  • Hongyan Ni
    • 1
  • LeiLei Zhang
    • 1
  • Yue Xing
    • 1
  • Jiayan Fan
    • 1
  • Peng Li
    • 1
  • Tianyuan Li
    • 1
  • Renbing Jia
    • 1
  • Shengfang Ge
    • 1
  • He Zhang
    • 1
  • Xianqun Fan
    • 1
  1. 1.Department of Ophthalmology, Ninth People’s HospitalShanghai JiaoTong University School of MedicineShanghaiChina

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