Tumor Biology

, Volume 37, Issue 9, pp 12213–12221 | Cite as

Silencing of ST6GalNAc I suppresses the proliferation, migration and invasion of hepatocarcinoma cells through PI3K/AKT/NF-κB pathway

  • Xiao Yu
  • Qiang Wu
  • Liping Wang
  • Yujie Zhao
  • Qingqing Zhang
  • Qingtao Meng
  • Pawan
  • Shujing Wang
Original Article


ST6GalNAc I is the major Sialyl-Tn antigen (STn) synthase that is highly correlated with tumor invasion and metastasis. However, the roles and molecular mechanisms by which ST6GalNAc I mediates the malignant phenotypes of hepatocarcinoma cells still remain poorly unknown. In this study, we investigated the expression of STn and ST6GalNAc I in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have different metastatic potential, as compared with normal mouse liver cell line IAR-20. The results showed that the expression of ST6GalNAc I and STn in Hca-F and Hca-P cells was much higher than that in Hepa1-6 and IAR20 cells. Knockdown of ST6GalNAc I by shRNA in Hca-F cells significantly decreased the expression of STn and inhibited the growth of tumor cells in vitro and in vivo. This reduction of ST6GalNAc I expression also led to the decreased migration and invasion of Hca-F cells. Furthermore, we found that ST6GalNAc I knockdown inhibited the expression levels of PI3k, p-Akt473, p-Akt308, NF-κB, and their downstream molecules. Together, our results suggest a role of ST6GalNAc I in promoting the growth and invasion of hepatocarcinoma cells through regulating PI3K/AKT signaling, and ST6GalNAc I might be a promising marker for the prognosis and therapy of hepatocarcinoma.


ST6GalNAc I STn Hepatocarcinoma Proliferation Migration Invasion 



This work was supported by grants from the Natural Science Foundation of China (31470799) and the Natural Science Foundation of Liaoning Province (2014023032).

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Xiao Yu
    • 1
  • Qiang Wu
    • 2
  • Liping Wang
    • 2
  • Yujie Zhao
    • 2
  • Qingqing Zhang
    • 1
  • Qingtao Meng
    • 3
  • Pawan
    • 1
  • Shujing Wang
    • 2
  1. 1.Department of PathologyDalian Medical UniversityDalianChina
  2. 2.Department of Biochemistry and Molecular Biology, Institute of GlycobiologyDalian Medical UniversityDalianChina
  3. 3.Department of Surgery, The Third People’s Hospital of DalianAffiliated Hospital of Dalian Medical UniversityDalianChina

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