Interleukin-22 promotes papillary thyroid cancer cell migration and invasion through microRNA-595/Sox17 axis
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Interleukin-22 (IL-22) is an inflammatory cytokine mainly produced by activated Th17 and Th22 cells. The data presented here demonstrate that IL-22 induced the migration and invasion of papillary thyroid cancer (PTC) cells. MicroRNA expression analysis and functional studies indicated that IL-22-mediated migration and invasion is positively regulated by miR-595. Further mechanistic studies revealed that sex-determining region Y-box 17 (Sox17) is directly targeted by miR-595. We then demonstrated that IL-22 regulated migration and invasion of PTC cells via inhibiting Sox17 expression. Interestingly, in PTC cell lines and PTC tissues, expression of IL-22 and miR-595 was upregulated and Sox17 downregulated compared with normal thyroid, and their expression levels were closely correlated. Taken together, this present study suggests that IL-22 stimulation enhances the migration and invasion of PTC cells by regulating miR-595 and its target Sox17.
KeywordsInterleukin-22 miR-595 Sox17 Papillary thyroid cancer Migration Invasion
Compliance with ethical standards
All participants gave written informed consent to participate in the study. The study was conducted according to the principles of the Declaration of Helsinki and approved by the Institutional Review Board of the Hubei Cancer Hospital, in accordance with its guidelines for the protection of human subjects.
This work was supported by research grants from the Young Scientist Fund of the National Natural Science Foundation of China (81301428) and the Fundamental Research Funds for the Central Universities (2042015kf0188).
The funding agencies had no role in study design, data collection, or analysis, decision to publish, or preparation of the manuscript.
Conflicts of interest
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