Tumor Biology

, Volume 37, Issue 8, pp 11321–11329 | Cite as

miR-522 contributes to cell proliferation of hepatocellular carcinoma by targeting DKK1 and SFRP2

Original Article

Abstract

The morbidity and mortality of hepatocellular carcinoma (HCC) is very high, finding new therapeutic targets are critical for HCC treatment. miR-522 has been demonstrated to be upregulated in HCC tissues, but its role in HCC progression remains to be elucidated. In this report, we found miR-522 was upregulated in HCC cells and tissues, miR-522 overexpression promoted cell proliferation, colony formation, and cell cycle progression, whereas knockdown of miR-522 reduced these effects. We also analyzed the expression of several key cell cycle regulatory proteins and found overexpression of miR-522-inhibited cell cycle inhibitors p21 and p27 expression and enhanced cyclin D1 expression and the level of Rb phosphorylation, vice versa. These suggested miR-522-accelerated G1/S transition. DKK1 (dickkopf-1) and SFRP2 (secreted frizzled-related protein 2) were the targets of miR-522, their expression was inversely with miR-522 in HCC tissues. DKK1 and SFRP2 the antagonists of Wnt signaling, suggesting miR-522-promoted HCC progression through activating Wnt signaling. miR-522 might be a valuable target for HCC therapy.

Keywords

miR-522 HCC DKK1 and SFRP2 Cell proliferation 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (no. 81160311, 81572429, and 81560477).

Compliance with ethical standards

Conflicts of interest

None

Supplementary material

13277_2016_4995_Fig7_ESM.gif (17 kb)
Supplemental Figure 1

Knockdown of miR-522 inhibited cell proliferation rate in LO2. (GIF 17 kb)

13277_2016_4995_MOESM1_ESM.tif (46 kb)
High resolution image (TIF 46 kb)
13277_2016_4995_Fig8_ESM.gif (289 kb)
Supplemental Figure 2

Western blot determined the expression of cell cycle inhibitors p21 and p27 and cell cycle promoter Cyclin D1 and the phosporylation level of Rb in LO2 with miR-522 knockdown. α-Tubulin was used as the loading control. (GIF 288 kb)

13277_2016_4995_MOESM2_ESM.tif (427 kb)
High resolution image (TIF 426 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  1. 1.Department of Hepatobiliary SurgeryAffiliated Hospital of Guiyang Medical CollegeGuiyangChina
  2. 2.Department of Hepatic-Biliary-Pancreatic SurgeryHubei Cancer HospitalWuhanChina

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