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Tumor Biology

, Volume 37, Issue 8, pp 11259–11266 | Cite as

The conglomeration of diagnostic, prognostic and therapeutic potential of serum miR-199a and its association with clinicopathological features in epithelial ovarian cancer

  • Mariyam Zuberi
  • Imran Khan
  • Gauri Gandhi
  • P. C. Ray
  • Alpana Saxena
Original Article

Abstract

Epithelial ovarian cancer (EOC) is the most lethal cause of morbidity and mortality worldwide. miRNA deregulation evinces a remarkable role in ovarian cancer tumorigenesis. miRNA-199a (miR-199a) is known to be involved in cancer development and progression. Although miR-199a has been studied in various cell types, its correlation with clinicopathological features in EOC has not been documented. In this study, we identified the clinicopathological hallmarks which might be perturbed due to the downregulation of serum miR-199a in EOC. Seventy serum samples from histopathologically confirmed EOC patients and 70 controls were collected. Total RNA from serum was isolated by Trizol method, polyadenylated and reverse transcribed into cDNA. Expression level of miR-199a was detected by using miRNA qRT-PCR. Relative expression was determined with matched controls using U6 snRNA as reference. Level of miR-199a expression was compared with distinct clinicopathological features. Expression of miR-199a was found to be significantly downregulated in comparison with matched normal controls. The expression level of miR-199a was found to be significantly associated with tumor stage, lymph node metastasis, and distal metastasis. Receiver operating characteristic (ROC) curve for diagnostic potential yielded significant area under the curve (AUC) with a considerable sensitivity and specificity. ROC curves for prognosis yielded significant AUCs for histological grade, distal metastasis, lymph node status, and survival. Our findings suggest that miR-199a downregulation might be a potential indicator for disease progression promoting the aggressive tumor progression and be identified as a diagnostic marker to predict the prognosis and survival in EOC patients.

Keywords

Epithelial ovarian cancer microRNA-199a serum biomarker diagnosis prognosis 

Abbreviations

EOC

Epithelial ovarian cancer

miR

microRNA

qRT-PCR

Quantitative real-time polymerase chain reaction

Notes

Acknowledgments

We gratefully acknowledge the assistance of University Grants Commission for supporting this work.

Compliance with Ethical Standards

Conflicts of interest

None.

Informed consent

Written informed consent was obtained from all individual participants included in the study.

Ethical approval

All procedures performed in this study involving human participants were approved and were in accordance with the institutional ethics committee at Maulana Azad Medical College, New Delhi.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Mariyam Zuberi
    • 1
  • Imran Khan
    • 2
  • Gauri Gandhi
    • 3
  • P. C. Ray
    • 1
  • Alpana Saxena
    • 1
  1. 1.Department of BiochemistryMaulana Azad Medical CollegeNew DelhiIndia
  2. 2.Department of SurgeryUniversity of Illinois at ChicagoChicagoUSA
  3. 3.Department of Gynaecology and ObstetricsLok Nayak HospitalNew DelhiIndia

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