Tumor Biology

, Volume 37, Issue 8, pp 10935–10941 | Cite as

Association between p21 Ser31Arg polymorphism and the development of cervical lesion in women infected with high risk HPV

  • Géssica Lima
  • Erinaldo Santos
  • Hildson Angelo
  • Micheline Oliveira
  • Sandra Heráclio
  • Fernanda Leite
  • Celso de Melo
  • Sergio Crovella
  • Maria Maia
  • Paulo Souza
Original Article


Infection by high-risk human papillomavirus (HR-HPV) and single nucleotide polymorphism (SNP) in genes involved in cell cycle control, as p21 and p27, are important factors in the development of different types of human cancers. This study aims at investigating whether both the p21 Ser31Arg and p27 V109G polymorphisms are associated with susceptibility to the development of cervical lesions in women HR-HPV positive. We analyzed 132 women HPV positive and with cervical lesions or CC and 154 healthy control (HPV negative and without cervical lesions). p21 Ser31Arg and p27 V109G polymorphisms were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and sequencing. The p21 31Arg allele was associated with susceptibility for the development of cervical lesions (P* = 0.0009), while p27 V109G polymorphism showed no significant differences for this association (P* = 0.89). However, the combined effect of the polymorphisms showed that the presence of the CC genotype (SNP p21 Ser31Arg) conferred protection for the development of cervical lesions (OR = 0.39). p21 Ser31Arg and p27 V109G polymorphisms were not associated with the grade of cervical lesions (CINI, CINII, and CINIII) or CC (P* > 0.05). The HR-HPV more frequent in this study were of 16 (57.6 %) and 18 (37.1 %) types; however, no association was observed when both polymorphisms and risk factors analyzed were compared (P* > 0.05). Our findings suggest a possible association between p21 Ser31tabArg polymorphism and susceptibility to the development of cervical lesions in women from Pernambuco. Brazil.


SNPs Cell cycle Cervical cancer HPV 


Compliance with ethical standards

Conflict of interest


Supplementary material

13277_2016_4979_MOESM1_ESM.doc (100 kb)
ESM 1 (DOC 94 kb)
13277_2016_4979_MOESM2_ESM.doc (111 kb)
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13277_2016_4979_MOESM3_ESM.doc (347 kb)
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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Géssica Lima
    • 1
  • Erinaldo Santos
    • 2
  • Hildson Angelo
    • 3
    • 4
  • Micheline Oliveira
    • 5
  • Sandra Heráclio
    • 6
  • Fernanda Leite
    • 7
  • Celso de Melo
    • 8
  • Sergio Crovella
    • 3
    • 9
  • Maria Maia
    • 2
    • 7
  • Paulo Souza
    • 1
    • 2
    • 7
  1. 1.Post-Graduate Program in Cellular and Molecular Biology AppliedUniversity of Pernambuco (UPE)RecifeBrazil
  2. 2.Post-Graduate Program in Tropical Animal ScienceRural Federal University of Pernambuco (UFRPE)RecifeBrazil
  3. 3.Post-Graduate Program in GeneticFederal University of Pernambuco (UFPE)RecifeBrazil
  4. 4.Federal Institute of Pernambuco – Campus Garanhuns (IFPE/Garanhuns)GaranhunsBrazil
  5. 5.Post-Graduate Program in Biological ScienceFederal University of Pernambuco (UFPE)RecifeBrazil
  6. 6.Departament of Lower Genital Tract Pathology, Women’s Healthcare CenterInstituto de Medicina Integral Prof. Fernando Figueira (IMIP)RecifeBrazil
  7. 7.Department of BiologyRural Federal University of Pernambuco (UFRPE)RecifeBrazil
  8. 8.Departament of PhysicsFederal University of Pernambuco (UFPE)RecifeBrazil
  9. 9.Departament of GeneticsFederal University of Pernambuco (UFPE)RecifeBrazil

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