Tumor Biology

, Volume 37, Issue 8, pp 10851–10860 | Cite as

Long noncoding RNA ENST00000434223 suppressed tumor progression in non-small cell lung cancer

  • Xiaofei Chai
  • Xiangyun Ye
  • Yongping Song
Original Article


In spite of the fact that the great progress has been made in the treatment of non-small cell lung cancer (NSCLC), the prognosis of NSCLC remains comparatively dismal. Therefore, it is of great value to identify novel effective diagnostic biomarkers and therapeutic targets of NSCLC. Emerging evidence has demonstrated the vital roles of long noncoding RNAs (lncRNAs) in cancer development. ENST00000434223 was recently identified as a lncRNA that is downregulated in early stage lung adenocarcinoma in a profiling study. However, little is known about its role in the development of NSCLC. In the present study, we found that ENST00000434223 was greatly downregulated in cancer tissues compared to adjacent normal tissues. ENST00000434223 overexpression suppressed the proliferation and migration in NSCLC cell lines in vitro. Moreover, ENST00000434223 overexpression reversed the epithelial-mesenchymal transition in NSCLC cell line. Our study suggests that ENST00000434223 may be a potential biomarker and a therapeutic target of NSCLC.


Non-small cell lung cancer Long noncoding RNA ENST00000434223 Epithelial-mesenchymal-transition 


Compliance with ethical standards

Understanding and written consent of each subject were obtained. This study was approved by the Human Ethics Committee of Chest Hospital at JIAOTONG University (Shanghai, China).

The animal care and experimental protocols were approved by the institutional guidelines of Zhengzhou University.

Conflicts of interest



  1. 1.
    Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA Cancer J Clin. 2013;63(1):11–30.CrossRefPubMedGoogle Scholar
  2. 2.
    Boolell V, Alamgeer M, Watkins DN, Ganju V. The evolution of therapies in non-small cell lung cancer. Cancers (Basel). 2015;7(3):1815–46.CrossRefGoogle Scholar
  3. 3.
    Vijayvergia N, Shah PC, Denlinger CS. Survivorship in non-small cell lung cancer: challenges faced and steps forward. J Natl Compr Canc Netw. 2015;13(9):1151–61.PubMedGoogle Scholar
  4. 4.
    Wang P, Xue Y, Han Y, Lin L, Wu C, Xu S, et al. The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation. Science. 2014;344(6181):310–3.CrossRefPubMedGoogle Scholar
  5. 5.
    Li Z, Chao TC, Chang KY, Lin N, Patil VS, Shimizu C, et al. The long noncoding RNA THRIL regulates TNFα expression through its interaction with hnRNPL. Proc Natl Acad Sci U S A. 2014;111(3):1002–7.CrossRefPubMedGoogle Scholar
  6. 6.
    Guo G, Kang Q, Zhu X, Chen Q, Wang X, Chen Y, et al. A long noncoding RNA critically regulates Bcr-Abl-mediated cellular transformation by acting as a competitive endogenous RNA. Oncogene. 2015;34(14):1768–79.CrossRefPubMedGoogle Scholar
  7. 7.
    Xue Y, Ma G, Zhang Z, Hua Q, Chu H, Tong N, et al. A novel antisense long noncoding RNA regulates the expression of MDC1 in bladder cancer. Oncotarget. 2015;6(1):484–93.PubMedGoogle Scholar
  8. 8.
    Tang J, Zhuo H, Zhang X, Jiang R, Ji J, Deng L, et al. A novel biomarker Linc00974 interacting with KRT19 promotes proliferation and metastasis in hepatocellular carcinoma. Cell Death Dis. 2014;5, e1549.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Ma MZ, Chu BF, Zhang Y, Weng MZ, Qin YY, Gong W, et al. Long non-coding RNA CCAT1 promotes gallbladder cancer development via negative modulation of miRNA-218-5p. Cell Death Dis. 2015;6, e1583.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Wang Y, He L, Du Y, Zhu P, Huang G, Luo J, et al. The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling. Cell Stem Cell. 2015;16(4):413–25.CrossRefPubMedGoogle Scholar
  11. 11.
    Wang P, Lu S, Mao H, Bai Y, Ma T, Cheng Z, et al. Identification of biomarkers for the detection of early stage lung adenocarcinoma by microarray profiling of long noncoding RNAs. Lung Cancer. 2015;88(2):147–53.CrossRefPubMedGoogle Scholar
  12. 12.
    Xie C, Yuan J, Li H, Li M, Zhao G, Bu D, et al. NONCODEv4: exploring the world of long non-coding RNA genes. Nucleic Acids Res. 2014;42(Database issue):D98–103.CrossRefPubMedGoogle Scholar
  13. 13.
    Kong L, Zhang Y, Ye ZQ, Liu XQ, Zhao SQ, Wei L, et al. CPC: assess the protein-coding potential of transcripts using sequence features and support vector machine. Nucleic Acids Res. 2007;35:W345–9. Web Server issue.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Lin MF, Jungreis I, Kellis M. PhyloCSF: a comparative genomics method to distinguish protein coding and non-coding regions. Bioinformatics. 2011;27(13):i275–82.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Fickett JW. Recognition of protein coding regions in DNA sequences. Nucleic Acids Res. 1982;10(17):5303–18.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Li L, Li W. Epithelial-mesenchymal transition in human cancer: comprehensive reprogramming of metabolism, epigenetics, and differentiation. Pharmacol Ther. 2015;150:33–46.CrossRefPubMedGoogle Scholar
  17. 17.
    Aparicio LA, Blanco M, Castosa R, Concha Á, Valladares M, Calvo L, et al. Clinical implications of epithelial cell plasticity in cancer progression. Cancer Lett. 2015;366(1):1–10.CrossRefPubMedGoogle Scholar
  18. 18.
    Bogachek MV, De Andrade JP, Weigel RJ. Regulation of epithelial-mesenchymal transition through SUMOylation of transcription factors. Cancer Res. 2015;75(1):11–5.CrossRefPubMedGoogle Scholar
  19. 19.
    Califano R, Morgillo F, De Mello RA, Mountzios G. Role of mesenchymal-epithelial transition amplification in resistance to anti-epidermal growth factor receptor agents. Ann Transl Med. 2015;3(6):81.PubMedPubMedCentralGoogle Scholar
  20. 20.
    Chen B, Tan Z, Gao J, Wu W, Liu L, Jin W, et al. Hyperphosphorylation of ribosomal protein S6 predicts unfavorable clinical survival in non-small cell lung cancer. J Exp Clin Cancer Res. 2015;34(1):126.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Vincent EE, Sergushichev A, Griss T, Gingras MC, Samborska B, Ntimbane T, et al. Mitochondrial phosphoenolpyruvate carboxykinase regulates metabolic adaptation and enables glucose-independent tumor growth. Mol Cell. 2015;60(2):195–207.CrossRefPubMedGoogle Scholar
  22. 22.
    DeNicola GM, Chen PH, Mullarky E, Sudderth JA, Hu Z, Wu D, et al. NRF2 regulates serine biosynthesis in non-small cell lung cancer. Nat Genet. 2015. doi: 10.1038/ng.3421.PubMedPubMedCentralGoogle Scholar
  23. 23.
    Fang L, Cai J, Chen B, Wu S, Li R, Xu X, et al. Aberrantly expressed miR-582-3p maintains lung cancer stem cell-like traits by activating Wnt/β-catenin signalling. Nat Commun. 2015;6:8640.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  1. 1.School of Basic Medical SciencesZhengzhou UniversityZhengzhouChina
  2. 2.Department of Pathologythe Affiliated Cancer Hospital to Zhengzhou UniversityZhengzhouChina
  3. 3.Department of Shanghai Lung Tumor Clinical Medical Centre, Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghaiChina
  4. 4.Department of Haematologythe Affiliated Cancer Hospital to Zhengzhou UniversityZhengzhouChina

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