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Tumor Biology

, Volume 37, Issue 8, pp 10871–10876 | Cite as

Long noncoding RNA SPRY4-IT1 promotes esophageal squamous cell carcinoma cell proliferation, invasion, and epithelial-mesenchymal transition

  • Fei Cui
  • Duoguang Wu
  • Xiaotian He
  • Wenjian Wang
  • Jingle Xi
  • Minghui Wang
Original Article

Abstract

The biology of esophageal squamous cell carcinoma (ESCC) remains poorly understood. Long noncoding RNAs (lncRNAs) are found to be dysregulated in a variety of cancers, including ESCC. SPRY4-IT1 has been recently revealed as oncogenic regulator or tumor suppressors in different cancers; however, whether SPRY4-IT1 is involved in ESCC remains poorly understood. To investigate the role of SPRY4-IT1 in ESCC, we evaluated the SPRY4-IT1 expression levels in a series of ESCC patients and a panel of ESCC cell line using qRT-PCR. CCK8 and colony formation assay were performed to assess the effect of SPRY4-IT1siRNA on cell proliferation, migration, and invasion of ESCC cell lines. SPRY4-IT1 expression was upregulated in ESCC tissues and the higher expression of SPRY4-IT1 was significantly correlated with tumor grade, depth of invasion, and lymph node metastasis. Moreover, silencing of SPRY4-IT1 expression inhibited ESCC cell proliferation, colony formation, migration, and invasion. Therefore, our study indicates that SPRY4-IT1 promotes proliferation and migration of ESCC cells and is a potential oncogene of ESCC.

Keywords

SPRY4-IT1 Long noncoding RNA Esophageal squamous cell carcinoma EMT 

Notes

Acknowledgments

This work was supported by Grant [2013] 163 from the Key Laboratory of Malignant Tumor Molecular Mechanism and Translational Medicine of Guangzhou Bureau of Science and information Technology; Grant KLB09001 from the Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education institutes, National Natural Science Foundation of China grant (No. 81372567), the Natural Science Foundation of Guangdong Province, China (No. 2014A030310450), and Director of Nanfang Hospital Foundation (No. 2013B018)

Compliance with ethical standards

Conflicts of interest

None

References

  1. 1.
    Matsushima K, Isomoto H, Yamaguchi N, Inoue N, Machida H, Nakayama T, et al. MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells. J Transl Med. 2011;9:30.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Nagaraja V, Eslick GD. Forthcoming prognostic markers for esophageal cancer: a systematic review and meta-analysis. J Gastrointest Oncol. 2014;5:67–76.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Wu J, Wu X, Liang W, Chen C, Zheng L, An H. Clinicopathological and prognostic significance of chemokine receptor CXCR4 overexpression in patients with esophageal cancer: a meta-analysis. Tumour Biol. 2014;35(4):3709–15.CrossRefPubMedGoogle Scholar
  4. 4.
    Guohong Z, Min S, Duenmei W, Songnian H, Min L, Jinsong L, et al. Genetic heterogeneity of oesophageal cancer in high-incidence areas of southern and northern China. PLoS One. 2010;5:e9668.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Wang KC, Chang HY. Molecular mechanisms of long noncoding RNAs. Mol Cell. 2011;43:904–14.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Guttman M, Donaghey J, Carey BW, Garber M, Grenier JK, Munson G, et al. LincRNAs act in the circuitry controlling pluripotency and differentiation. Nature. 2011;477:295–300.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Tsai MC, Manor O, Wan Y, Mosammaparast N, Wang JK, Lan F, et al. Long noncoding RNA as modular scaffold of histone modification complexes. Science. 2010;329:689–93.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Qiu MT, Hu JW, Yin R, Xu L. Long noncoding RNA: an emerging paradigm of cancer research. Tumour Biol. 2013;34:613–20.CrossRefPubMedGoogle Scholar
  9. 9.
    Khaitan D, Dinger ME, Mazar J, Crawford J, Smith MA, Mattick JS, et al. The melanoma-upregulated long noncoding RNA SPRY4-IT1 modulates apoptosis and invasion. Cancer Res. 2011;71:3852–62.CrossRefPubMedGoogle Scholar
  10. 10.
    Zou Y, Jiang Z, Yu X, Sun M, Zhang Y, Zuo Q, et al. Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV/neo. PLoS One. 2013;8(11):e79598.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Mazar J, Zhao W, Khalil AM, Lee B, Shelley J, Govindarajan SS. The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells. Oncotarget. 2014;5:8959–69.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Matsushima K, Isomoto H, Kohno S, Nakao K. MicroRNAs and esophageal squamous cell carcinoma. Digestion. 2010;82:138–44.CrossRefPubMedGoogle Scholar
  13. 13.
    Chen FJ, Sun M, Li SQ, Wu QQ, Ji L, Liu ZL, et al. Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis. Mol Carcin. 2012;52:908–15.CrossRefGoogle Scholar
  14. 14.
    Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 2010;464:1071–6.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Xie M, Nie FQ, Sun M, Xia R, Liu YW, Zhou P, et al. Decreased long noncoding RNA SPRY4-IT1 contributing to gastric cancer cell metastasis partly via affecting epithelial-mesenchymal transition. J Transl Med. 2015;13:250.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Peng W, Wu G, Fan H, Wu J, Feng J. Long noncoding RNA SPRY4-IT1 predicts poor patient prognosis and promotes tumorigenesis in gastric cancer. Tumour Biol. 2015;36(9):6751–8.CrossRefPubMedGoogle Scholar
  17. 17.
    Sun M, Liu XH, Lu KH, Nie FQ, Xia R, Kong R, et al. EZH2-mediated epigenetic suppression of long noncoding RNA SPRY4-IT1 promotes NSCLC cell proliferation and metastasis by affecting the epithelial-mesenchymal transition. Cell Death Dis. 2014;5:e1298.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Fei Cui
    • 1
  • Duoguang Wu
    • 2
  • Xiaotian He
    • 2
  • Wenjian Wang
    • 2
  • Jingle Xi
    • 1
  • Minghui Wang
    • 2
  1. 1.Department of Oncology, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of Thoracic Surgery, Sun Yat-Sen memorial hospitalSun Yat-Sen UniversityGuangzhouChina

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