Tumor Biology

, Volume 37, Issue 8, pp 10871–10876 | Cite as

Long noncoding RNA SPRY4-IT1 promotes esophageal squamous cell carcinoma cell proliferation, invasion, and epithelial-mesenchymal transition

  • Fei Cui
  • Duoguang Wu
  • Xiaotian He
  • Wenjian Wang
  • Jingle Xi
  • Minghui Wang
Original Article


The biology of esophageal squamous cell carcinoma (ESCC) remains poorly understood. Long noncoding RNAs (lncRNAs) are found to be dysregulated in a variety of cancers, including ESCC. SPRY4-IT1 has been recently revealed as oncogenic regulator or tumor suppressors in different cancers; however, whether SPRY4-IT1 is involved in ESCC remains poorly understood. To investigate the role of SPRY4-IT1 in ESCC, we evaluated the SPRY4-IT1 expression levels in a series of ESCC patients and a panel of ESCC cell line using qRT-PCR. CCK8 and colony formation assay were performed to assess the effect of SPRY4-IT1siRNA on cell proliferation, migration, and invasion of ESCC cell lines. SPRY4-IT1 expression was upregulated in ESCC tissues and the higher expression of SPRY4-IT1 was significantly correlated with tumor grade, depth of invasion, and lymph node metastasis. Moreover, silencing of SPRY4-IT1 expression inhibited ESCC cell proliferation, colony formation, migration, and invasion. Therefore, our study indicates that SPRY4-IT1 promotes proliferation and migration of ESCC cells and is a potential oncogene of ESCC.


SPRY4-IT1 Long noncoding RNA Esophageal squamous cell carcinoma EMT 



This work was supported by Grant [2013] 163 from the Key Laboratory of Malignant Tumor Molecular Mechanism and Translational Medicine of Guangzhou Bureau of Science and information Technology; Grant KLB09001 from the Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education institutes, National Natural Science Foundation of China grant (No. 81372567), the Natural Science Foundation of Guangdong Province, China (No. 2014A030310450), and Director of Nanfang Hospital Foundation (No. 2013B018)

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Fei Cui
    • 1
  • Duoguang Wu
    • 2
  • Xiaotian He
    • 2
  • Wenjian Wang
    • 2
  • Jingle Xi
    • 1
  • Minghui Wang
    • 2
  1. 1.Department of Oncology, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of Thoracic Surgery, Sun Yat-Sen memorial hospitalSun Yat-Sen UniversityGuangzhouChina

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