RETRACTED ARTICLE: Overexpression of SphK1 enhances cell proliferation and invasion in triple-negative breast cancer via the PI3K/AKT signaling pathway
- 1k Downloads
Sphingosine kinase 1 (SphK1) expression is elevated in various cancers and is associated with shorter survival times for patients. However, the molecular mechanism of SphK1 up-regulation in triple-negative breast cancer (TNBC) remains unclear. In this study, we assayed the expression level of SphK1 in TNBC tissues by qRT-PCR and immunohistochemistry. The level of S1P was quantified by ELISA in the serum of TNBC patients. Our results found that the levels of SphK1 and S1P were significantly increased in TNBC patients compared with normal control. Furthermore, knockdown of SphK1 with siRNA decreased TNBC cell proliferation and inhibited cell migration/invasion. These data suggest that SphK1 has an important role in TNBC and presents an attractive therapeutic target for the treatment for TNBC.
KeywordsSphingosine kinase 1 Sphingosine-1-phosphate Triple-negative breast cancer Chemotherapy AKT PI3K
Compliance with ethical standards
Our study was permitted by Center’s Ethnics Committee of Shaanxi Provincial Tumor Hospital, and informed consent was obtained from all patients.
Conflicts of interest
- 18.Tan SS, Khin LW, Wong L, Yan B, Ong CW, Datta A, et al. Sphingosine kinase 1 promotes malignant progression in colon cancer and independently predicts survival of patients with colon cancer by competing risk approach in South Asian population. Clin Transl Gastroenterol. 2014;5, e51.CrossRefPubMedPubMedCentralGoogle Scholar