Clinical impact of circulating microRNAs as blood-based marker in childhood acute lymphoblastic leukemia
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Aberrant microRNA (miRNA) expression participates in childhood acute lymphoblastic leukemia (ALL). This study aimed to investigate the expression of miRNA-100, miRNA-196a, and miRNA-146a among childhood ALL and study their correlation with other hematological parameters and different phenotypes. Peripheral blood mononuclear cells (PMNCs) were obtained from 85 childhood ALL and 25 healthy children for the detection of miRNA expression using quantitative real-time PCR. Significant higher median levels were reported for ALL compared to control children. The diagnostic efficacy for miRNA-146a was superior as both sensitivity and specificity were absolute. A significant correlation was observed between higher expression of miRNA-100 and lower platelet and lymphocyte counts; high expression of miRNA-146a showed significant correlation with low total leukocyte count (TLC) and lymphocyte counts. Significant relation was reported between studied miRNAs and different phenotyping. miRNA-100, miRNA-196a, and miRNA-146a have significant role in childhood ALL leukemogenesis, and they may be useful as biological diagnostic molecular markers.
KeywordsMiRNA RT-PCR Childhood ALL
Authors contributed equally.
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Conflicts of interest
- 1.Crazzolara R, Bendall L. Emerging treatments in acute lymphoblastic leukemia. CurrCancer DrugTargets. 2009;9:19–31.Google Scholar
- 4.Vitale A, Guarini A, Chiaretti S, Foa R. The changing scene of adult acute lymphoblastic leukemia. CurrOpinOncol. 2006;18:652–9.Google Scholar
- 5.Nguyen HT, Tian G, Murph MM. Molecular epigenetics in the management of ovarian cancer: are we investigating a rational clinical promise? Frontiers in Oncology, doi: 10.3389/fonc.2014.00071 , 2014
- 7.Fan Y, Fan J, Huang L, Ye M, Huang Z, Wang Y, et al. Increased expression of microRNA-196a predicts poor prognosis in human ovarian carcinoma. Int J ClinExpPathol. 2015;8:4132–7.Google Scholar
- 9.Cui J. MiR-16 family as potential diagnostic biomarkers for cancer: a systematic review and meta-analysis. Int J ClinExp Med. 2015;8:1703–14.Google Scholar
- 14.Yu H, Lindsay J, Feng ZP, Frankenberg S, Hu Y,Carone D, Shaw G,Pask AJ, O’NeillR, Papenfuss AT, Renfree MB. Evolution of coding and non-coding genes in HOX clusters of a marsupial. BMC Genomics 2012, doi: 10.1186/1471-2164-13-251
- 22.Schotte D, Lange-Turenhout EAM, Stumpel DJPM,Stam RW, JGCAM Buijs-GladdinesJGCAM, MeijerinkJPP, Pieters R,Boer MLD. “Expression of miR-196b is not exclusively MLL-driven but is especially linked to activation of HOXA genes in pediatric acute lymphoblastic leukemia,” Haematologica. 2010; 95:1675–1682. Stam RW, JGCAM Buijs-GladdinesJGCAM, MeijerinkJPP, Pieters R,Boer MLDGoogle Scholar
- 23.Duyu M, Durmaz B, Gunduz C, Vergin C, KarapinarYD, Aksoylar S, Kavakli K, Cetingul N, Irken G, Yaman Y, Ozkinay F, Cogulu O. Prospective evaluation of whole genome microRNA expression profiling in childhood acute lymphoblastic leukemia. BioMed Research International Volume 2014, Article ID 967585, 7 pages http://dx.doi.org/ 10.1155/2014/967585
- 25.Justiniano SE, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda JM, et al. Fcγreceptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies. J Biol Chem. 2013;288:26800–9.CrossRefPubMedPubMedCentralGoogle Scholar