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Tumor Biology

, Volume 37, Issue 8, pp 10545–10551 | Cite as

miR-448 suppressed gastric cancer proliferation and invasion by regulating ADAM10

  • Xuesong Wu
  • Haoran Tang
  • Guobin Liu
  • Hui Wang
  • Jie Shu
  • Feng Sun
Original Article

Abstract

MicroRNAs (miRNAs) are a class of short, noncoding RNAs that act a crucial role in tumor development. Previous studies showed that miR-448 expression was deregulated in many tumors. However, the role of miR-448 in gastric cancer (GC) remains unknown. In our study, we demonstrated that miR-448 expression was downregulated in GC tissues compared with the corresponding nontumor tissues. We also showed that miR-448 expression was downregulated in GC cell lines. Ectopic expression of miR-448 suppressed GC cell proliferation, colony formation, and invasion. Moreover, we identified A Disintegrin And Metalloproteinases 10 (ADAM10) as a direct target gene of miR-448 in GC cell. ADAM10 expression was upregulated in GC tissues and cells. Furthermore, the expression level of miR-448 was negatively correlated with the expression level of ADAM10 in GC tissues. Moreover, ADAM10 overexpression rescued the effect of miR-448-mediated GC cell proliferation, colony formation, and invasion. These results demonstrated that miR-448 might play as a tumor suppressor miRNA partly through targeting ADAM10 expression.

Keywords

Gastric cancer microRNAs miR-448 ADAM10 

Notes

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Xuesong Wu
    • 1
  • Haoran Tang
    • 1
  • Guobin Liu
    • 2
  • Hui Wang
    • 3
  • Jie Shu
    • 1
  • Feng Sun
    • 1
  1. 1.Department of Gastrointenstinal SurgeryThe Second Affiliated Hospital of Kunming Medical UniversityKunmingChina
  2. 2.Department of GastroenterologyThe Second Affiliated Hospital of Kunming Medical UniversityKunmingChina
  3. 3.Department of GastroenterologyThe Affiliated YanAn Hospital of Kunming Medical UniversityKunmingChina

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