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Tumor Biology

, Volume 37, Issue 8, pp 11267–11278 | Cite as

Antigen-specific T cell response from dendritic cell vaccination using side population cell-associated antigens targets hepatocellular carcinoma

  • Xiao Li
  • Zhuochao Zhang
  • Guoying Lin
  • Yuanxing Gao
  • Zhen Yan
  • Heliang Yin
  • Bingyi Sun
  • Fangyuan Wang
  • Haijun Zhang
  • Hong Chen
  • Dayong Cao
Original Article
  • 346 Downloads

Abstract

Dendritic cell (DC) vaccination targeting cancer stem cells is an effective way to suppress tumor progression and reduce the metastasis and recurrence. In the present study, we explored the suitability of side population (SP) cells as source of antigens for DC vaccination against hepatocellular carcinoma (HCC) in a mouse model. In this study, we identified the “stem-like” characteristics of SP cells in the MHCC97 and Hepa 1-6 HCC cell lines. We found that SP cells express high levels of tumor-associated antigens and MHC class I molecules. Although loading with cell lysates did not change the characteristics of DCs, SP cell lysate-pulsed DCs induced antigen-specific T cell responses, including T cell proliferation and increased IFN-γ production by stimulated CD8+ T cells. We investigated the cytotoxicity of T cells stimulated by SP cell lysate-pulsed DCs in nude mice co-injected with MHCC97 cells. To mimic the in vivo environment, we also confirmed the result in mouse HCC cell line Hepa 1-6 induced tumor-bearing C57/BL6 immune competent mice, and we demonstrated that vaccination with DCs loaded with Hepa 1-6 SP cell lysates could induce a T cell response in vivo and suppress the tumor growth. Our results may have applications for anti-HCC immunotherapy by targeting the cancer stem cells and may provide new insight for cancer vaccines.

Keywords

Dendritic cells Side population cells Cancer stem cells Hepatocellular carcinoma Cytotoxic T lymphocytes 

Notes

Acknowledgments

We thank Dr. Ben Ma and Wei Sun for their help in the experiment. The experiment was supported by National Natural Science Foundation (81201926).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  1. 1.Department of Hepatobiliary Surgery, Xijing HospitalFourth Military Medical UniversityXi’anChina
  2. 2.The Second Department of General SurgeryThe First Hospital of Qiqihaer CityQiqihaerChina
  3. 3.Integrative Research CenterThe First Hospital of Qiqihaer CityQiqihaerChina

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