Frequencies of EGFR single nucleotide polymorphisms in non-small cell lung cancer patients and healthy individuals in the Republic of Serbia: a preliminary study
- 129 Downloads
The purpose of this study was to determine the frequencies of EGFR −216G>T, −191C>A, and 181946C>T in Serbian non-small cell lung cancer (NSCLC) patients, as well as to compare it with healthy individuals, in order to assess their potential importance for lung cancer in Serbia. The study involved 56 NSCLC patients and 53 unrelated healthy volunteers, and genotyping was performed on DNA samples obtained from formalin-fixed paraffin-embedded lung tumor tissue and blood, respectively. This was the first time to show genotype frequencies of those single nucleotide polymorphisms for this study group from the territory of the Republic of Serbia. There was very strong evidence of association between age and death due to lung cancer (Pearson chi-square = 43.439, df = 2, p < 0,001), as well as between ever smoking and death due to lung cancer (Pearson chi-square = 31.727, df = 1, p < 0.001). When dominant genetic model (GG vs. GT+TT) was used for −216G>T, we have found significant association (p = 0.012) between −216GG genotype and NSCLC patients within smokers’ subgroup. So, carriers of −216GG genotype had higher risk (OR = 4.33, 95 % CI = 1.324–14.179) than noncarriers (GT and TT) for developing non-small cell lung cancer in our patients.
KeywordsEpidermal growth factor receptor Non-small cell lung cancer Single nucleotide polymorphism
The study was financially supported by the Ministry of Science, Republic of Serbia, 175056.
Compliance with ethical standards
Conflict of interests
The study was approved by local ethics committees.
Written informed consent was obtained from all study subjects.
- 16.Fukuoka M, Yano S, Giaccone G, Tamura T, Nakagawa K, Douillard JY, et al. Final results from a phase II trial of ZD1839 (‘Iressa’) for patients with advanced non-small-cell lung cancer (IDEAL 1). Proc Am Soc Clin Oncol. 2002;21:298a.Google Scholar
- 17.Ling WH, Lee SC. Inter-ethnic differences-how important is it in cancer treatment? AnnAcad Med Singapore. 2011;40(8):356–61.Google Scholar
- 21.Nomura M, Shigematsu H, Li L, Suzuki M, Takahashi T, Estess P, et al. Polymorphisms, mutations, and amplification of the EGFR gene in non-small cell lung cancers. PloSMed. 2007;4, e125.Google Scholar
- 24.Excoffier L, Laval G, Schneider S. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online. 2005;1:47–50.Google Scholar
- 25.Demographic Yearbook in the Republic of Serbia, 2013; ISSN 0084–4357; Published by: Statistical Office of the Republic of Serbia, Belgrade, 5 Milana Rakića St; For publishers: Prof. Dragan Vukmirovic, Ph.D, Director; 1–363Google Scholar
- 29.Reference SNP (refSNP) Cluster report rs 712829; Short Genetic Variations Database. [Internet] (Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=712829), accessed: January, 23, 2016. a
- 30.Reference SNP (refSNP) Cluster report rs 712830; Short Genetic Variations Database. [Internet] (Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=712830), accessed: January, 23, 2016. b
- 31.Reference SNP (refSNP) Cluster report rs 2293347; Short Genetic Variations Database. [Internet] (Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=2293347), accessed: January, 23, 2016. c
- 37.Gibson GJ, Loddenkemper R, Sibille Y, Lundback B. The European lung white book: respiratory health and disease in Europe, European Respiratory Society, 01.09.2013. 224–237.Google Scholar