Tumor Biology

, Volume 37, Issue 8, pp 10479–10486 | Cite as

Frequencies of EGFR single nucleotide polymorphisms in non-small cell lung cancer patients and healthy individuals in the Republic of Serbia: a preliminary study

  • Jasmina Obradović
  • Natasa Djordjević
  • Natasa Tošic
  • Jasminka Mrdjanović
  • Biljana Stanković
  • Jelena Stanić
  • Bojan Zarić
  • Branislav Perin
  • Sonja Pavlović
  • Vladimir Jurišić
Original Article


The purpose of this study was to determine the frequencies of EGFR −216G>T, −191C>A, and 181946C>T in Serbian non-small cell lung cancer (NSCLC) patients, as well as to compare it with healthy individuals, in order to assess their potential importance for lung cancer in Serbia. The study involved 56 NSCLC patients and 53 unrelated healthy volunteers, and genotyping was performed on DNA samples obtained from formalin-fixed paraffin-embedded lung tumor tissue and blood, respectively. This was the first time to show genotype frequencies of those single nucleotide polymorphisms for this study group from the territory of the Republic of Serbia. There was very strong evidence of association between age and death due to lung cancer (Pearson chi-square = 43.439, df = 2, p < 0,001), as well as between ever smoking and death due to lung cancer (Pearson chi-square = 31.727, df = 1, p < 0.001). When dominant genetic model (GG vs. GT+TT) was used for −216G>T, we have found significant association (p = 0.012) between −216GG genotype and NSCLC patients within smokers’ subgroup. So, carriers of −216GG genotype had higher risk (OR = 4.33, 95 % CI = 1.324–14.179) than noncarriers (GT and TT) for developing non-small cell lung cancer in our patients.


Epidermal growth factor receptor Non-small cell lung cancer Single nucleotide polymorphism 



The study was financially supported by the Ministry of Science, Republic of Serbia, 175056.

Compliance with ethical standards

Conflict of interests


Ethical standards

The study was approved by local ethics committees.

Informed consent

Written informed consent was obtained from all study subjects.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Jasmina Obradović
    • 1
  • Natasa Djordjević
    • 2
  • Natasa Tošic
    • 3
  • Jasminka Mrdjanović
    • 4
  • Biljana Stanković
    • 3
  • Jelena Stanić
    • 5
  • Bojan Zarić
    • 5
  • Branislav Perin
    • 5
  • Sonja Pavlović
    • 3
  • Vladimir Jurišić
    • 2
  1. 1.Institute of Biology and Ecology, Faculty of ScienceUniversity of KragujevacKragujevacSerbia
  2. 2.Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  3. 3.Institute of Molecular Genetics and Genetic EngineeringUniversity of BelgradeBelgradeSerbia
  4. 4.Institute for Oncology of VojvodinaSremska KamenicaSerbia
  5. 5.Institute for Pulmonary Diseases of Vojvodina, Clinic for Pulmonary Oncology, Faculty of MedicineUniversity of Novi SadSremska KamenicaSerbia

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