UCA1 functions as a competing endogenous RNA to suppress epithelial ovarian cancer metastasis
Urothelial cancer associated 1 (UCA1) is an example of functional long noncoding RNAs involved in many biologic processes. However, little is known about the association between UCA1 expression and metastasis in epithelial ovarian cancer (EOC). Findings of this study confirmed that not only UCA1 was aberrantly upregulated in EOC tissues and cells, but also correlated with status of lymph node metastasis and FIGO stage. Furthermore, univariate and multivariate analyses showed that UCA1 was a prognostic factor for overall survival in EOC patients. In vitro, knockdown of UCA1 reduced the invasion and migration ability of EOC cells. The results showed that UCA1 could function as an endogenous sponge by directly binding to miR-485-5p. Depletion of UCA1 was involved in the downregulation of matrix metallopeptidase 14 (MMP14) expression, a target gene of miR-485-5p. In conclusion, our work indicates that UCA1 is a new prognostic biomarker for EOC, establishing a novel connection among UCA1, miR-485-5p, and MMP14 in EOC metastasis.
KeywordsEpithelial ovarian cancer (EOC) LncRNA-UCA1 Competing endogenous RNA MMP14
We thank Dr. Jinsong Liu (University of Texas M.D. Anderson Cancer Center) for revising the manuscript carefully. This work was supported by the National Nature Science Foundation of China (81472442, 81272871), Jiangsu Province Medical Key Talent Grant (2011).
Compliance with ethical standards
Conflicts of interest
- 27.Tripathi V, Shen Z, Chakraborty A, Giri S, Freier SM, Wu X, et al. Long noncoding RNA MALAT1 controls cell cycle progression by regulating the expression of oncogenic transcription factor B-MYB. Plos Genet. 2013;9(3):e1003368. doi: 10.1371/journal.pgen.1003368.CrossRefPubMedPubMedCentralGoogle Scholar
- 32.Jo Y, Yeon J, Kim HJ, Lee ST. Analysis of tissue inhibitor of metalloproteinases-2 effect on pro-matrix metalloproteinase-2 activation by membrane-type 1 matrix metalloproteinase using baculovirus/insect-cell expression system. Biochem J. 2000;345(Pt 3):511–9.CrossRefPubMedPubMedCentralGoogle Scholar