Tumor Biology

, Volume 37, Issue 8, pp 10209–10218 | Cite as

Clinical significance of fibroblast growth factor receptor 2 expression in patients with residual rectal cancer after preoperative chemoradiotherapy: relationship with KRAS or BRAF mutations and MSI status

  • Ghilsuk Yoon
  • Hwayoung Lee
  • Jae-Hoon Kim
  • Keun Hur
  • An Na Seo
Original Article

Abstract

This study was designed to determine the prognostic impact and clinical significance of FGFR2 in residual disease after preoperative chemoradiotherapy (CRT) in patients with rectal cancer. The surgical specimens of 145 patients with residual rectal cancer after preoperative CRT were analyzed. To evaluate FGFR2 expression, immunohistochemistry was performed on whole section tissues. KRAS exon 2 (codon 12 and 13), BRAF V600E mutational status, and microsatellite instability (MSI) were determined using polymerase chain reactions. Of the eligible 141 patients, FGFR2 over-expression was observed in 75.9 % (n = 107) and was correlated with perineural invasion (P = 0.005) and inferior tumor regression grading (TRG) (P = 0.009). However, FGFR2 expression had no relationship with KRAS and BRAF mutation results or with MSI results. On univariate analysis, FGFR2 over-expression was significantly associated with worse rectal cancer-specific survival (RCSS) (P = 0.005) and disease-free survival (DFS) (P = 0.035). However, multivariate analysis revealed that FGFR2 over-expression was not independently associated with RCSS and DFS (all P > 0.05). Although FGFR2 over-expression did not independently influence patient outcome, FGFR2 over-expression was associated with worse prognosis and inferior TRG. Our data may aid in understanding the therapeutic approaches targeting FGFR2 in patients with residual rectal cancer after preoperative CRT.

Keywords

Rectal cancer Preoperative chemoradiotherapy Fibroblast growth factor receptor 2 Prognosis KRAS Microsatellite instability 

Notes

Acknowledgments

This work was supported by Biomedical Research Institute grant, Kyungpook National University Hospital (2015).

Compliance with ethical standards

Conflicts of interest

None

Supplementary material

13277_2016_4899_Fig3_ESM.jpg (158 kb)
Figure S1

Kaplan-Meier Survival curve for disease-free survival and rectal cancer-specific survival of patients with residual rectal cancer after preoperative chemoradiotherapy according to FGFR2 expression in (a and b) the KRAS wild-type subgroup and (c and d) the KRAS mutational subgroup. (JPG 158 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Ghilsuk Yoon
    • 1
    • 2
  • Hwayoung Lee
    • 2
  • Jae-Hoon Kim
    • 3
  • Keun Hur
    • 4
  • An Na Seo
    • 1
    • 2
    • 3
  1. 1.Department of PathologyKyungpook National University Medical CenterDaeguSouth Korea
  2. 2.Department of PathologyKyungpook National University School of MedicineDaeguSouth Korea
  3. 3.Department of PathologyKyungpook National University HospitalDaeguSouth Korea
  4. 4.Department of Biochemistry and Cell Biology, Cell and Matrix Research InstituteKyungpook National University School of MedicineDaeguSouth Korea

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